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Article: Immediately implantable extracellular matrix-enriched osteoinductive hydrogel-laden 3D-printed scaffold for promoting vascularized bone regeneration in vivo

TitleImmediately implantable extracellular matrix-enriched osteoinductive hydrogel-laden 3D-printed scaffold for promoting vascularized bone regeneration in vivo
Authors
Keywords3D hybrid model
3D printing
Biomacromolecules
Bone tissue engineering
Polycaprolactone
Issue Date2022
Citation
Materials and Design, 2022, v. 219, article no. 110801 How to Cite?
AbstractReconstruction of patient-specific scaffolds to repair uniquely shaped bone defects remains a major clinical challenge in tissue engineering. Recently, three-dimensional (3D) printed scaffolds have received considerable attention as a promising technology for the rapid generation of custom shapes. However, synthetic polymers commonly used for 3D printing, such as polycaprolactone (PCL), lack the biological capacity to mimic native extracellular matrix functions to support cell growth and differentiation into desired tissues. We described the preparation and characterization of a 3D hybrid model for bone tissue engineering that comprises an extracellular matrix (ECM)-enriched hydrogel embedded in a PCL scaffold. The human bone marrow-derived mesenchymal stem cell–derived matrisome (BMTS) was utilized as a source of ECM-enriched biomacromolecules, and scaffold biocompatibility was evaluated in vitro using human bone marrow-derived mesenchymal stem cells (BM-MSCs). The 3D hybrid model exhibited excellent BM-MSC viability and osteogenic activity in vitro in both two-dimensional (2D) and 3D cultures. Furthermore, bone remodeling was evaluated by in vivo through a rat calvarial defect model; notably, the fabricated 3D hybrid model effectively enhanced vascularized bone regeneration. Therefore, this promising BMTS-based 3D hybrid model might serve as an excellent bone tissue-engineered scaffold for use in orthopedic applications.
Persistent Identifierhttp://hdl.handle.net/10722/324220
ISSN
2023 Impact Factor: 7.6
2023 SCImago Journal Rankings: 1.684
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSeo Lee, Jae-
dc.contributor.authorNah, Haram-
dc.contributor.authorLee, Donghyun-
dc.contributor.authorAn, Sang Hyun-
dc.contributor.authorKo, Wan Kyu-
dc.contributor.authorLee, Sang Jin-
dc.contributor.authorYeon Lee, Seung-
dc.contributor.authorMin Park, Kyung-
dc.contributor.authorBok Lee, Jung-
dc.contributor.authorYi, Hyeong joong-
dc.contributor.authorKeun Kwon, Il-
dc.contributor.authorChoi, Kyu Sun-
dc.contributor.authorNyoung Heo, Dong-
dc.date.accessioned2023-01-13T03:02:18Z-
dc.date.available2023-01-13T03:02:18Z-
dc.date.issued2022-
dc.identifier.citationMaterials and Design, 2022, v. 219, article no. 110801-
dc.identifier.issn0264-1275-
dc.identifier.urihttp://hdl.handle.net/10722/324220-
dc.description.abstractReconstruction of patient-specific scaffolds to repair uniquely shaped bone defects remains a major clinical challenge in tissue engineering. Recently, three-dimensional (3D) printed scaffolds have received considerable attention as a promising technology for the rapid generation of custom shapes. However, synthetic polymers commonly used for 3D printing, such as polycaprolactone (PCL), lack the biological capacity to mimic native extracellular matrix functions to support cell growth and differentiation into desired tissues. We described the preparation and characterization of a 3D hybrid model for bone tissue engineering that comprises an extracellular matrix (ECM)-enriched hydrogel embedded in a PCL scaffold. The human bone marrow-derived mesenchymal stem cell–derived matrisome (BMTS) was utilized as a source of ECM-enriched biomacromolecules, and scaffold biocompatibility was evaluated in vitro using human bone marrow-derived mesenchymal stem cells (BM-MSCs). The 3D hybrid model exhibited excellent BM-MSC viability and osteogenic activity in vitro in both two-dimensional (2D) and 3D cultures. Furthermore, bone remodeling was evaluated by in vivo through a rat calvarial defect model; notably, the fabricated 3D hybrid model effectively enhanced vascularized bone regeneration. Therefore, this promising BMTS-based 3D hybrid model might serve as an excellent bone tissue-engineered scaffold for use in orthopedic applications.-
dc.languageeng-
dc.relation.ispartofMaterials and Design-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subject3D hybrid model-
dc.subject3D printing-
dc.subjectBiomacromolecules-
dc.subjectBone tissue engineering-
dc.subjectPolycaprolactone-
dc.titleImmediately implantable extracellular matrix-enriched osteoinductive hydrogel-laden 3D-printed scaffold for promoting vascularized bone regeneration in vivo-
dc.typeArticle-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1016/j.matdes.2022.110801-
dc.identifier.scopuseid_2-s2.0-85131724983-
dc.identifier.volume219-
dc.identifier.spagearticle no. 110801-
dc.identifier.epagearticle no. 110801-
dc.identifier.eissn1873-4197-
dc.identifier.isiWOS:000975786200001-

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