File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Anti-neuroinflammatory gold nanocomplex loading ursodeoxycholic acid following spinal cord injury

TitleAnti-neuroinflammatory gold nanocomplex loading ursodeoxycholic acid following spinal cord injury
Authors
KeywordsDrug delivery systems
Gold nanoparticles
Neuroinflammation
Spinal cord injuries
Ursodeoxycholic acid
Issue Date2019
Citation
Chemical Engineering Journal, 2019, v. 375, article no. 122088 How to Cite?
AbstractSpinal cord injury (SCI) causes permanent nerve damage by disturbing axon regeneration from pro-inflammations and still remains a serious worldwide problem. Ursodeoxycholic acid (UDCA) has been reported that it can serve as an anti-neuroinflammatory drug such as Methylprednisolone (MP). However, the therapeutic effect of UDCA is limited due to its hydrophobic property. In this study, we aimed to investigate the anti-neuroinflammatory effects of gold nanoparticles (GNPs) loading UDCA (GNP-UDCA complex) following SCI. For this, we conjugated with β-cyclodextrin (β-CD) on the GNP's surface and included UDCA in β-CD by sonicating. We confirmed that the GNP-UDCA complex successfully synthesized. A mechanical SCI was imposed on rats and we injected GNP-UDCA complex three times after injury. After SCI, motor function and the lesions were significantly improved in the GNP-UDCA complex group than those in MP group and UDCA group. This complex significantly decreased pro-inflammatory cytokines and increased anti-inflammatory cytokines in terms of mRNA and protein levels than MP and UDCA. In addition, GNP-UDCA complex significantly suppressed the phosphorylation of extracellular signal-regulated kinase (p-ERK) and c-Jun N-terminal kinase (p-JNK) in the mitogen-activated protein kinase (MAPK) pathway than MP and UDCA. Finally, the complex significantly exhibited the expression of inducible nitric oxide synthase (iNOS). It also induced the expression of arginase-1 (Arg-1), anti-inflammatory marker, at the injured sites more than that in the saline group. In conclusion, this study demonstrates the anti-neuroinflammatory effects of GNP-UDCA complex in SCI and suggests that the GNP-UDCA complex can be an alternative drug system for SCI cases.
Persistent Identifierhttp://hdl.handle.net/10722/324094
ISSN
2023 Impact Factor: 13.3
2023 SCImago Journal Rankings: 2.852
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorKim, Seong Jun-
dc.contributor.authorKo, Wan Kyu-
dc.contributor.authorHeo, Dong Nyoung-
dc.contributor.authorLee, Sang Jin-
dc.contributor.authorLee, Donghyun-
dc.contributor.authorHeo, Min-
dc.contributor.authorHan, In Bo-
dc.contributor.authorKwon, Il Keun-
dc.contributor.authorSohn, Seil-
dc.date.accessioned2023-01-13T03:01:28Z-
dc.date.available2023-01-13T03:01:28Z-
dc.date.issued2019-
dc.identifier.citationChemical Engineering Journal, 2019, v. 375, article no. 122088-
dc.identifier.issn1385-8947-
dc.identifier.urihttp://hdl.handle.net/10722/324094-
dc.description.abstractSpinal cord injury (SCI) causes permanent nerve damage by disturbing axon regeneration from pro-inflammations and still remains a serious worldwide problem. Ursodeoxycholic acid (UDCA) has been reported that it can serve as an anti-neuroinflammatory drug such as Methylprednisolone (MP). However, the therapeutic effect of UDCA is limited due to its hydrophobic property. In this study, we aimed to investigate the anti-neuroinflammatory effects of gold nanoparticles (GNPs) loading UDCA (GNP-UDCA complex) following SCI. For this, we conjugated with β-cyclodextrin (β-CD) on the GNP's surface and included UDCA in β-CD by sonicating. We confirmed that the GNP-UDCA complex successfully synthesized. A mechanical SCI was imposed on rats and we injected GNP-UDCA complex three times after injury. After SCI, motor function and the lesions were significantly improved in the GNP-UDCA complex group than those in MP group and UDCA group. This complex significantly decreased pro-inflammatory cytokines and increased anti-inflammatory cytokines in terms of mRNA and protein levels than MP and UDCA. In addition, GNP-UDCA complex significantly suppressed the phosphorylation of extracellular signal-regulated kinase (p-ERK) and c-Jun N-terminal kinase (p-JNK) in the mitogen-activated protein kinase (MAPK) pathway than MP and UDCA. Finally, the complex significantly exhibited the expression of inducible nitric oxide synthase (iNOS). It also induced the expression of arginase-1 (Arg-1), anti-inflammatory marker, at the injured sites more than that in the saline group. In conclusion, this study demonstrates the anti-neuroinflammatory effects of GNP-UDCA complex in SCI and suggests that the GNP-UDCA complex can be an alternative drug system for SCI cases.-
dc.languageeng-
dc.relation.ispartofChemical Engineering Journal-
dc.subjectDrug delivery systems-
dc.subjectGold nanoparticles-
dc.subjectNeuroinflammation-
dc.subjectSpinal cord injuries-
dc.subjectUrsodeoxycholic acid-
dc.titleAnti-neuroinflammatory gold nanocomplex loading ursodeoxycholic acid following spinal cord injury-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.cej.2019.122088-
dc.identifier.scopuseid_2-s2.0-85067940671-
dc.identifier.volume375-
dc.identifier.spagearticle no. 122088-
dc.identifier.epagearticle no. 122088-
dc.identifier.isiWOS:000483341000154-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats