File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Isolation and functional characterization of P-TEFb-associated factors that control general and HIV-1 transcriptional elongation

TitleIsolation and functional characterization of P-TEFb-associated factors that control general and HIV-1 transcriptional elongation
Authors
KeywordsAffinity-purification
HIV-1 transcription
P-TEFb
P-TEFb-associated factors
Tat-transactivation
Issue Date2011
Citation
Methods, 2011, v. 53, n. 1, p. 85-90 How to Cite?
AbstractOriginally identified as a factor crucial for RNA polymerase (Pol) II transcriptional elongation of cellular genes, the P-TEFb kinase was subsequently shown to also serve as a specific host co-factor required for HIV-1 transcription. Recruited by either the bromodomain protein Brd4 to cellular promoters for general transcription or the HIV-1 Tat protein to the viral LTR for activated HIV-1 transcription, P-TEFb stimulates the processivity of Pol II through phosphorylating the C-terminal domain of Pol II and a pair of negative elongation factors, leading to the synthesis of full-length transcripts. However, abundant evidence indicates that P-TEFb does not act alone in the cell and that all of its known biological functions are likely mediated through the interactions with various regulators. Although a number of P-TEFb-associated factors have already been identified, there are likely more yet to be discovered. Given that P-TEFb plays an essential role in HIV-1 transcription, a major challenge facing the field is to identify all the P-TEFb-associated factors and determine how they may modulate Tat-transactivation and HIV-1 replication. Described here is a set of experimental procedures that have not only enabled us to isolate and identify several P-TEFb-associated factors, but also provided the means to characterize their biochemical functions in HIV-1 transcriptional control. In light of the recent demonstrations that transcriptional elongation plays a much more important role in controlling metazoan gene expression than previously thought, the techniques presented here will also be useful for analyzing Pol II elongation of cellular genes. © 2010 Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/323849
ISSN
2023 Impact Factor: 4.2
2023 SCImago Journal Rankings: 1.162
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChen, Ruichuan-
dc.contributor.authorLiu, Min-
dc.contributor.authorZhang, Kai-
dc.contributor.authorZhou, Qiang-
dc.date.accessioned2023-01-13T02:59:45Z-
dc.date.available2023-01-13T02:59:45Z-
dc.date.issued2011-
dc.identifier.citationMethods, 2011, v. 53, n. 1, p. 85-90-
dc.identifier.issn1046-2023-
dc.identifier.urihttp://hdl.handle.net/10722/323849-
dc.description.abstractOriginally identified as a factor crucial for RNA polymerase (Pol) II transcriptional elongation of cellular genes, the P-TEFb kinase was subsequently shown to also serve as a specific host co-factor required for HIV-1 transcription. Recruited by either the bromodomain protein Brd4 to cellular promoters for general transcription or the HIV-1 Tat protein to the viral LTR for activated HIV-1 transcription, P-TEFb stimulates the processivity of Pol II through phosphorylating the C-terminal domain of Pol II and a pair of negative elongation factors, leading to the synthesis of full-length transcripts. However, abundant evidence indicates that P-TEFb does not act alone in the cell and that all of its known biological functions are likely mediated through the interactions with various regulators. Although a number of P-TEFb-associated factors have already been identified, there are likely more yet to be discovered. Given that P-TEFb plays an essential role in HIV-1 transcription, a major challenge facing the field is to identify all the P-TEFb-associated factors and determine how they may modulate Tat-transactivation and HIV-1 replication. Described here is a set of experimental procedures that have not only enabled us to isolate and identify several P-TEFb-associated factors, but also provided the means to characterize their biochemical functions in HIV-1 transcriptional control. In light of the recent demonstrations that transcriptional elongation plays a much more important role in controlling metazoan gene expression than previously thought, the techniques presented here will also be useful for analyzing Pol II elongation of cellular genes. © 2010 Elsevier Inc.-
dc.languageeng-
dc.relation.ispartofMethods-
dc.subjectAffinity-purification-
dc.subjectHIV-1 transcription-
dc.subjectP-TEFb-
dc.subjectP-TEFb-associated factors-
dc.subjectTat-transactivation-
dc.titleIsolation and functional characterization of P-TEFb-associated factors that control general and HIV-1 transcriptional elongation-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.ymeth.2010.04.005-
dc.identifier.pmid20385240-
dc.identifier.scopuseid_2-s2.0-78651469063-
dc.identifier.volume53-
dc.identifier.issue1-
dc.identifier.spage85-
dc.identifier.epage90-
dc.identifier.eissn1095-9130-
dc.identifier.isiWOS:000286708000013-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats