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Article: HIV-1 Tat targets microtubules to induce apoptosis, a process promoted by the pro-apoptotic Bcl-2 relative Bim

TitleHIV-1 Tat targets microtubules to induce apoptosis, a process promoted by the pro-apoptotic Bcl-2 relative Bim
Authors
KeywordsApoptosis
Bim
HIV-1
Microtubule
Tat
Issue Date2002
Citation
EMBO Journal, 2002, v. 21, n. 24, p. 6801-6810 How to Cite?
AbstractDepletion of CD4+ T cells is the hallmark of HIV infection and AIDS progression. In addition to the direct killing of the viral-infected cells, HIV infection also leads to increased apoptosis of predominantly uninfected bystander cells. This is mediated in part through the HIV-1 Tat protein, which is secreted by the infected cells and taken up by uninfected cells. Using an affinity-purification approach, a specific and direct interaction of Tat with tubulin and polymerized microtubules has been detected. This interaction does not affect the secretion and uptake of Tat, but is critical for Tat to induce apoptosis. Tat binds tubulin/microtubules through a four-amino-acid subdomain of its conserved core region, leading to the alteration of microtubule dynamics and activation of a mitochondria-dependent apoptotic pathway. Bim, a proapoptotic Bcl-2 relative and a transducer of death signals initiated by perturbation of microtubule dynamics, facilitates the Tat-induced apoptosis. Our findings reveal a strategy by which Tat induces apoptosis by targeting the microtubule network. Thus HIV-1 Tat joins a growing list of pathogen-derived proteins that target the cytoskeleton of host cells.
Persistent Identifierhttp://hdl.handle.net/10722/323781
ISSN
2023 Impact Factor: 9.4
2023 SCImago Journal Rankings: 5.489
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChen, Dan-
dc.contributor.authorWang, Michael-
dc.contributor.authorZhou, Sharleen-
dc.contributor.authorZhou, Qiang-
dc.date.accessioned2023-01-13T02:59:17Z-
dc.date.available2023-01-13T02:59:17Z-
dc.date.issued2002-
dc.identifier.citationEMBO Journal, 2002, v. 21, n. 24, p. 6801-6810-
dc.identifier.issn0261-4189-
dc.identifier.urihttp://hdl.handle.net/10722/323781-
dc.description.abstractDepletion of CD4+ T cells is the hallmark of HIV infection and AIDS progression. In addition to the direct killing of the viral-infected cells, HIV infection also leads to increased apoptosis of predominantly uninfected bystander cells. This is mediated in part through the HIV-1 Tat protein, which is secreted by the infected cells and taken up by uninfected cells. Using an affinity-purification approach, a specific and direct interaction of Tat with tubulin and polymerized microtubules has been detected. This interaction does not affect the secretion and uptake of Tat, but is critical for Tat to induce apoptosis. Tat binds tubulin/microtubules through a four-amino-acid subdomain of its conserved core region, leading to the alteration of microtubule dynamics and activation of a mitochondria-dependent apoptotic pathway. Bim, a proapoptotic Bcl-2 relative and a transducer of death signals initiated by perturbation of microtubule dynamics, facilitates the Tat-induced apoptosis. Our findings reveal a strategy by which Tat induces apoptosis by targeting the microtubule network. Thus HIV-1 Tat joins a growing list of pathogen-derived proteins that target the cytoskeleton of host cells.-
dc.languageeng-
dc.relation.ispartofEMBO Journal-
dc.subjectApoptosis-
dc.subjectBim-
dc.subjectHIV-1-
dc.subjectMicrotubule-
dc.subjectTat-
dc.titleHIV-1 Tat targets microtubules to induce apoptosis, a process promoted by the pro-apoptotic Bcl-2 relative Bim-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/emboj/cdf683-
dc.identifier.pmid12486001-
dc.identifier.scopuseid_2-s2.0-12244304872-
dc.identifier.volume21-
dc.identifier.issue24-
dc.identifier.spage6801-
dc.identifier.epage6810-
dc.identifier.isiWOS:000179952000018-

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