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Article: Epigallocatechin Gallate Inhibits Macaque SEVI-Mediated Enhancement of SIV or SHIV Infection

TitleEpigallocatechin Gallate Inhibits Macaque SEVI-Mediated Enhancement of SIV or SHIV Infection
Authors
Keywordsamyloid fibrils
EGCG
macaque
SEVI
SHIV
SIV
Issue Date2017
Citation
Journal of Acquired Immune Deficiency Syndromes, 2017, v. 75, n. 2, p. 232-240 How to Cite?
AbstractBackground: Human semen contains a factor that can enhance HIV infection up to 10 5 -fold in cultures. This factor is termed semen-derived enhancer of virus infection (SEVI) and is composed of proteolytic fragments (PAP248-286) from prostatic acid phosphatase in semen. In this study, we examined whether macaque SEVI can facilitate simian immunodeficiency virus (SIV) or chimeric simian/human immunodeficiency virus (SHIV) infection. We also studied the effect of epigallocatechin gallate (EGCG) on macaque SEVI-mediated SIV or SHIV enhancement. Methods: SIV or SHIV was mixed with different concentrations of macaque SEVI in the presence or absence of EGCG. The mixture was added to cultures of TZM-bl cells or macaque PBMCs. The effect of EGCG on macaque SEVI was measured by Congo-red staining assay and thioflavin T (ThT) fluorescence assay and was visualized by a transmission electron microscope. Results: We identified that there is one amino acid difference at the site of 277 between human PAP248-286 and macaque PAP248-286. Macaque SEVI significantly enhanced SIV or SHIV infection of TZM-bl cells and macaque PBMCs. EGCG could block macaque SEVI-mediated enhancement of SIV or SHIV infection. Mechanistically, EGCG could degrade the formation of macaque SEVI amyloid fibrils that facilitates HIV attachment to the target cells. Conclusions: The finding that macaque SEVI could enhance SIV or SHIV infection indicates the possibility to use the macaque SEVI in vivo studies with the macaque models. In addition, future studies are necessary to examine whether EGCG can be used as an effective microbicide for preventing SIV or SHIV mucosal transmission.
Persistent Identifierhttp://hdl.handle.net/10722/321720
ISSN
2023 Impact Factor: 2.9
2023 SCImago Journal Rankings: 1.225
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhou, Run Hong-
dc.contributor.authorGuo, Le-
dc.contributor.authorLiu, Jin Biao-
dc.contributor.authorLiu, Hang-
dc.contributor.authorHou, Wei-
dc.contributor.authorMa, Tong Cui-
dc.contributor.authorWang, Xu-
dc.contributor.authorWu, Jian Guo-
dc.contributor.authorYe, Li-
dc.contributor.authorHo, Wen Zhe-
dc.contributor.authorLi, Jie Liang-
dc.date.accessioned2022-11-03T02:21:00Z-
dc.date.available2022-11-03T02:21:00Z-
dc.date.issued2017-
dc.identifier.citationJournal of Acquired Immune Deficiency Syndromes, 2017, v. 75, n. 2, p. 232-240-
dc.identifier.issn1525-4135-
dc.identifier.urihttp://hdl.handle.net/10722/321720-
dc.description.abstractBackground: Human semen contains a factor that can enhance HIV infection up to 10 5 -fold in cultures. This factor is termed semen-derived enhancer of virus infection (SEVI) and is composed of proteolytic fragments (PAP248-286) from prostatic acid phosphatase in semen. In this study, we examined whether macaque SEVI can facilitate simian immunodeficiency virus (SIV) or chimeric simian/human immunodeficiency virus (SHIV) infection. We also studied the effect of epigallocatechin gallate (EGCG) on macaque SEVI-mediated SIV or SHIV enhancement. Methods: SIV or SHIV was mixed with different concentrations of macaque SEVI in the presence or absence of EGCG. The mixture was added to cultures of TZM-bl cells or macaque PBMCs. The effect of EGCG on macaque SEVI was measured by Congo-red staining assay and thioflavin T (ThT) fluorescence assay and was visualized by a transmission electron microscope. Results: We identified that there is one amino acid difference at the site of 277 between human PAP248-286 and macaque PAP248-286. Macaque SEVI significantly enhanced SIV or SHIV infection of TZM-bl cells and macaque PBMCs. EGCG could block macaque SEVI-mediated enhancement of SIV or SHIV infection. Mechanistically, EGCG could degrade the formation of macaque SEVI amyloid fibrils that facilitates HIV attachment to the target cells. Conclusions: The finding that macaque SEVI could enhance SIV or SHIV infection indicates the possibility to use the macaque SEVI in vivo studies with the macaque models. In addition, future studies are necessary to examine whether EGCG can be used as an effective microbicide for preventing SIV or SHIV mucosal transmission.-
dc.languageeng-
dc.relation.ispartofJournal of Acquired Immune Deficiency Syndromes-
dc.subjectamyloid fibrils-
dc.subjectEGCG-
dc.subjectmacaque-
dc.subjectSEVI-
dc.subjectSHIV-
dc.subjectSIV-
dc.titleEpigallocatechin Gallate Inhibits Macaque SEVI-Mediated Enhancement of SIV or SHIV Infection-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1097/QAI.0000000000001361-
dc.identifier.pmid28328549-
dc.identifier.scopuseid_2-s2.0-85015952414-
dc.identifier.volume75-
dc.identifier.issue2-
dc.identifier.spage232-
dc.identifier.epage240-
dc.identifier.eissn1077-9450-
dc.identifier.isiWOS:000401166700019-

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