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Article: Assays to monitor autophagy in Drosophila

TitleAssays to monitor autophagy in Drosophila
Authors
KeywordsAtg8
Autophagosome
Autophagy
Drosophila
Fat body
Ref(2)P
Issue Date2014
Citation
Methods, 2014, v. 68, n. 1, p. 134-139 How to Cite?
AbstractThe term autophagy refers to the engulfment and degradation of cytoplasmic components within the lysosome. This process can benefit cells and organisms by removing damaged, superfluous, or harmful cellular components, and by generating a supply of recycled macromolecules that can support biosynthesis or energy production. Recent interest in autophagy has been driven by its potential role in several disease-related phenomena including neurodegeneration, cancer, immunity and aging. Drosophila provides a valuable animal model context for these studies, and work in this system has also begun to identify novel developmental and physiological roles of autophagy. Here, we provide an overview of methods for monitoring autophagy in Drosophila, with a special emphasis on the larval fat body. These methods can be used to investigate whether observed vesicles are of autophagic origin, to determine a relative rate of autophagic degradation, and to identify specific step(s) in the autophagic process in which a given gene functions. © 2014 Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/318568
ISSN
2023 Impact Factor: 4.2
2023 SCImago Journal Rankings: 1.162
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMauvezin, Caroline-
dc.contributor.authorAyala, Carlos-
dc.contributor.authorBraden, Christopher R.-
dc.contributor.authorKim, Jung-
dc.contributor.authorNeufeld, Thomas P.-
dc.date.accessioned2022-10-11T12:24:03Z-
dc.date.available2022-10-11T12:24:03Z-
dc.date.issued2014-
dc.identifier.citationMethods, 2014, v. 68, n. 1, p. 134-139-
dc.identifier.issn1046-2023-
dc.identifier.urihttp://hdl.handle.net/10722/318568-
dc.description.abstractThe term autophagy refers to the engulfment and degradation of cytoplasmic components within the lysosome. This process can benefit cells and organisms by removing damaged, superfluous, or harmful cellular components, and by generating a supply of recycled macromolecules that can support biosynthesis or energy production. Recent interest in autophagy has been driven by its potential role in several disease-related phenomena including neurodegeneration, cancer, immunity and aging. Drosophila provides a valuable animal model context for these studies, and work in this system has also begun to identify novel developmental and physiological roles of autophagy. Here, we provide an overview of methods for monitoring autophagy in Drosophila, with a special emphasis on the larval fat body. These methods can be used to investigate whether observed vesicles are of autophagic origin, to determine a relative rate of autophagic degradation, and to identify specific step(s) in the autophagic process in which a given gene functions. © 2014 Elsevier Inc.-
dc.languageeng-
dc.relation.ispartofMethods-
dc.subjectAtg8-
dc.subjectAutophagosome-
dc.subjectAutophagy-
dc.subjectDrosophila-
dc.subjectFat body-
dc.subjectRef(2)P-
dc.titleAssays to monitor autophagy in Drosophila-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.ymeth.2014.03.014-
dc.identifier.pmid24667416-
dc.identifier.scopuseid_2-s2.0-84901853505-
dc.identifier.volume68-
dc.identifier.issue1-
dc.identifier.spage134-
dc.identifier.epage139-
dc.identifier.eissn1095-9130-
dc.identifier.isiWOS:000337551300015-

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