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- Publisher Website: 10.1007/s10529-008-9776-4
- Scopus: eid_2-s2.0-52449103310
- PMID: 18575806
- WOS: WOS:000259412200004
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Article: Improved serum stability and biophysical properties of siRNAs following chemical modifications
Title | Improved serum stability and biophysical properties of siRNAs following chemical modifications |
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Authors | |
Keywords | 2′-Ribose modification A-form helix Melting temperature RNA interference siRNA Stability |
Issue Date | 2008 |
Citation | Biotechnology Letters, 2008, v. 30, n. 11, p. 1901-1908 How to Cite? |
Abstract | Small interfering RNAs (siRNAs) which mediate sequence-specific gene silencing through RNA interference have been harnessed for therapeutic applications. Here, we found combinations of modest 2′-ribose siRNA modifications that permit effective gene silencing and improve siRNA stability in human serum. Introduction of as few as two different or identical 2′-ribose modifications at a specific position of either strand, which has been previously suggested to suppress unintended off-target effects, enhances the thermal and thermodynamic stability of siRNA duplexes, and does not alter the overall A-form helical geometry that is required for silencing activity. Our results provide a useful template for the design for therapeutic siRNAs. © 2008 Springer Science+Business Media B.V. |
Persistent Identifier | http://hdl.handle.net/10722/318450 |
ISSN | 2023 Impact Factor: 2.0 2023 SCImago Journal Rankings: 0.519 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Cho, Ik Sang | - |
dc.contributor.author | Kim, Jung | - |
dc.contributor.author | Lim, Do Hwan | - |
dc.contributor.author | Ahn, Hee Chul | - |
dc.contributor.author | Kim, Hyunggee | - |
dc.contributor.author | Lee, Kang Bong | - |
dc.contributor.author | Lee, Young Sik | - |
dc.date.accessioned | 2022-10-11T12:23:47Z | - |
dc.date.available | 2022-10-11T12:23:47Z | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | Biotechnology Letters, 2008, v. 30, n. 11, p. 1901-1908 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | http://hdl.handle.net/10722/318450 | - |
dc.description.abstract | Small interfering RNAs (siRNAs) which mediate sequence-specific gene silencing through RNA interference have been harnessed for therapeutic applications. Here, we found combinations of modest 2′-ribose siRNA modifications that permit effective gene silencing and improve siRNA stability in human serum. Introduction of as few as two different or identical 2′-ribose modifications at a specific position of either strand, which has been previously suggested to suppress unintended off-target effects, enhances the thermal and thermodynamic stability of siRNA duplexes, and does not alter the overall A-form helical geometry that is required for silencing activity. Our results provide a useful template for the design for therapeutic siRNAs. © 2008 Springer Science+Business Media B.V. | - |
dc.language | eng | - |
dc.relation.ispartof | Biotechnology Letters | - |
dc.subject | 2′-Ribose modification | - |
dc.subject | A-form helix | - |
dc.subject | Melting temperature | - |
dc.subject | RNA interference | - |
dc.subject | siRNA | - |
dc.subject | Stability | - |
dc.title | Improved serum stability and biophysical properties of siRNAs following chemical modifications | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1007/s10529-008-9776-4 | - |
dc.identifier.pmid | 18575806 | - |
dc.identifier.scopus | eid_2-s2.0-52449103310 | - |
dc.identifier.volume | 30 | - |
dc.identifier.issue | 11 | - |
dc.identifier.spage | 1901 | - |
dc.identifier.epage | 1908 | - |
dc.identifier.eissn | 1573-6776 | - |
dc.identifier.isi | WOS:000259412200004 | - |