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- Publisher Website: 10.1016/j.bbrc.2008.04.118
- Scopus: eid_2-s2.0-43549116789
- PMID: 18454937
- WOS: WOS:000256319100036
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Article: Functional analysis of dicer-2 missense mutations in the siRNA pathway of Drosophila
Title | Functional analysis of dicer-2 missense mutations in the siRNA pathway of Drosophila |
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Authors | |
Keywords | Dicer-2 Drosophila Mutation R2D2 RNA interference siRNA |
Issue Date | 2008 |
Citation | Biochemical and Biophysical Research Communications, 2008, v. 371, n. 3, p. 525-530 How to Cite? |
Abstract | The Drosophila RNase III enzyme Dicer-2 processes double-stranded RNA (dsRNA) precursors into small interfering RNAs (siRNAs). It also interacts with the siRNA product and R2D2 protein to facilitate the assembly of an RNA-induced silencing complex (RISC) that mediates RNA interference. Here, we characterized six independent missense mutations in the dicer-2 gene. Four mutations (P8S, L188F, R269W, and P365L) in the DExH helicase domain reduced dsRNA processing activity. Two mutations were located within an RNase III domain. P1496L caused a loss of dsRNA processing activity comparable to a null dicer-2 mutation. A1453T strongly reduced both dsRNA processing and RISC activity, and decreased the levels of Dicer-2 and R2D2 proteins, suggesting that this mutation destabilizes Dicer-2. We also found that the carboxyl-terminal region of R2D2 is essential for Dicer-2 binding. These results provide further insight into the structure-function relationship of Dicer, which plays a critical role in the siRNA pathway. © 2008 Elsevier Inc. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/318447 |
ISSN | 2023 Impact Factor: 2.5 2023 SCImago Journal Rankings: 0.770 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Lim, Do Hwan | - |
dc.contributor.author | Kim, Jung | - |
dc.contributor.author | Kim, Sanguk | - |
dc.contributor.author | Carthew, Richard W. | - |
dc.contributor.author | Lee, Young Sik | - |
dc.date.accessioned | 2022-10-11T12:23:47Z | - |
dc.date.available | 2022-10-11T12:23:47Z | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | Biochemical and Biophysical Research Communications, 2008, v. 371, n. 3, p. 525-530 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | http://hdl.handle.net/10722/318447 | - |
dc.description.abstract | The Drosophila RNase III enzyme Dicer-2 processes double-stranded RNA (dsRNA) precursors into small interfering RNAs (siRNAs). It also interacts with the siRNA product and R2D2 protein to facilitate the assembly of an RNA-induced silencing complex (RISC) that mediates RNA interference. Here, we characterized six independent missense mutations in the dicer-2 gene. Four mutations (P8S, L188F, R269W, and P365L) in the DExH helicase domain reduced dsRNA processing activity. Two mutations were located within an RNase III domain. P1496L caused a loss of dsRNA processing activity comparable to a null dicer-2 mutation. A1453T strongly reduced both dsRNA processing and RISC activity, and decreased the levels of Dicer-2 and R2D2 proteins, suggesting that this mutation destabilizes Dicer-2. We also found that the carboxyl-terminal region of R2D2 is essential for Dicer-2 binding. These results provide further insight into the structure-function relationship of Dicer, which plays a critical role in the siRNA pathway. © 2008 Elsevier Inc. All rights reserved. | - |
dc.language | eng | - |
dc.relation.ispartof | Biochemical and Biophysical Research Communications | - |
dc.subject | Dicer-2 | - |
dc.subject | Drosophila | - |
dc.subject | Mutation | - |
dc.subject | R2D2 | - |
dc.subject | RNA interference | - |
dc.subject | siRNA | - |
dc.title | Functional analysis of dicer-2 missense mutations in the siRNA pathway of Drosophila | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.bbrc.2008.04.118 | - |
dc.identifier.pmid | 18454937 | - |
dc.identifier.scopus | eid_2-s2.0-43549116789 | - |
dc.identifier.volume | 371 | - |
dc.identifier.issue | 3 | - |
dc.identifier.spage | 525 | - |
dc.identifier.epage | 530 | - |
dc.identifier.eissn | 1090-2104 | - |
dc.identifier.isi | WOS:000256319100036 | - |