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- Publisher Website: 10.1104/pp.16.01009
- Scopus: eid_2-s2.0-84994607921
- PMID: 27663408
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Article: Comparative analysis of light-harvesting antennae and state transition in chlorina and cpSRP mutants
Title | Comparative analysis of light-harvesting antennae and state transition in chlorina and cpSRP mutants |
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Authors | |
Issue Date | 2016 |
Citation | Plant Physiology, 2016, v. 172, n. 3, p. 1519-1531 How to Cite? |
Abstract | State transitions in photosynthesis provide for the dynamic allocation of a mobile fraction of light-harvesting complex II (LHCII) to photosystem II (PSII) in state I and to photosystem I (PSI) in state II. In the state I-to-state II transition, LHCII is phosphorylated by STN7 and associates with PSI to favor absorption cross-section of PSI. Here, we used Arabidopsis (Arabidopsis thaliana) mutants with defects in chlorophyll (Chl) b biosynthesis or in the chloroplast signal recognition particle (cpSRP) machinery to study the flexible formation of PS-LHC supercomplexes. Intriguingly, we found that impaired Chl b biosynthesis in chlorina1-2 (ch1-2) led to preferentially stabilized LHCI rather than LHCII, while the contents of both LHCI and LHCII were equally depressed in the cpSRP43-deficient mutant (chaos). In view of recent findings on the modified state transitions in LHCI-deficient mutants (Benson et al., 2015), the ch1-2 and chaos mutants were used to assess the influence of varying LHCI/LHCII antenna size on state transitions. Under state II conditions, LHCII-PSI supercomplexes were not formed in both ch1-2 and chaos plants. LHCII phosphorylation was drastically reduced in ch1-2, and the inactivation of STN7 correlates with the lack of state transitions. In contrast, phosphorylated LHCII in chaos was observed to be exclusively associated with PSII complexes, indicating a lack of mobile LHCII in chaos. Thus, the comparative analysis of ch1-2 and chaos mutants provides new evidence for the flexible organization of LHCs and enhances our understanding of the reversible allocation of LHCII to the two photosystems. |
Persistent Identifier | http://hdl.handle.net/10722/316460 |
ISSN | 2023 Impact Factor: 6.5 2023 SCImago Journal Rankings: 2.101 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Wang, Peng | - |
dc.contributor.author | Grimm, Bernhard | - |
dc.date.accessioned | 2022-09-14T11:40:30Z | - |
dc.date.available | 2022-09-14T11:40:30Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | Plant Physiology, 2016, v. 172, n. 3, p. 1519-1531 | - |
dc.identifier.issn | 0032-0889 | - |
dc.identifier.uri | http://hdl.handle.net/10722/316460 | - |
dc.description.abstract | State transitions in photosynthesis provide for the dynamic allocation of a mobile fraction of light-harvesting complex II (LHCII) to photosystem II (PSII) in state I and to photosystem I (PSI) in state II. In the state I-to-state II transition, LHCII is phosphorylated by STN7 and associates with PSI to favor absorption cross-section of PSI. Here, we used Arabidopsis (Arabidopsis thaliana) mutants with defects in chlorophyll (Chl) b biosynthesis or in the chloroplast signal recognition particle (cpSRP) machinery to study the flexible formation of PS-LHC supercomplexes. Intriguingly, we found that impaired Chl b biosynthesis in chlorina1-2 (ch1-2) led to preferentially stabilized LHCI rather than LHCII, while the contents of both LHCI and LHCII were equally depressed in the cpSRP43-deficient mutant (chaos). In view of recent findings on the modified state transitions in LHCI-deficient mutants (Benson et al., 2015), the ch1-2 and chaos mutants were used to assess the influence of varying LHCI/LHCII antenna size on state transitions. Under state II conditions, LHCII-PSI supercomplexes were not formed in both ch1-2 and chaos plants. LHCII phosphorylation was drastically reduced in ch1-2, and the inactivation of STN7 correlates with the lack of state transitions. In contrast, phosphorylated LHCII in chaos was observed to be exclusively associated with PSII complexes, indicating a lack of mobile LHCII in chaos. Thus, the comparative analysis of ch1-2 and chaos mutants provides new evidence for the flexible organization of LHCs and enhances our understanding of the reversible allocation of LHCII to the two photosystems. | - |
dc.language | eng | - |
dc.relation.ispartof | Plant Physiology | - |
dc.title | Comparative analysis of light-harvesting antennae and state transition in chlorina and cpSRP mutants | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1104/pp.16.01009 | - |
dc.identifier.pmid | 27663408 | - |
dc.identifier.scopus | eid_2-s2.0-84994607921 | - |
dc.identifier.volume | 172 | - |
dc.identifier.issue | 3 | - |
dc.identifier.spage | 1519 | - |
dc.identifier.epage | 1531 | - |
dc.identifier.eissn | 1532-2548 | - |
dc.identifier.isi | WOS:000391172300014 | - |