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Book Chapter: Protoplast Isolation and Transfection in the Single-Cell C4 Species Bienertia sinuspersici

TitleProtoplast Isolation and Transfection in the Single-Cell C4 Species Bienertia sinuspersici
Authors
Lung, SCYogadasan, NYanagisawa, MChuong, SDX
Issue Date2022
PublisherHumana
Citation
Protoplast Isolation and Transfection in the Single-Cell C4 Species Bienertia sinuspersici. In Wang, K., Zhang, F. (Eds.), Protoplast Technology. Methods in Molecular Biology, v. 2464, p. 21-28. New York, NY: Humana, 2022 How to Cite?
DOI: http://dx.doi.org/10.1007/978-1-0716-2164-6_2
AbstractWe have developed an optimized protocol for isolating protoplasts from chlorenchyma cells of the single-cell C4 species Bienertia sinuspersici. The isolated protoplasts maintained the integrity of the unique single-cell C4 intracellular compartmentation of organelles as observed in chlorenchyma cells after cell wall digestion. Approximately over 80% of isolated protoplasts expressed the fusion reporter gene following the polyethylene glycol-mediated transfection procedures. Overall, fluorescent protein fusion tagged with various intraorganellular sorting signals validated the potential use of the transient gene expression system in subcellular localization and organelle dynamics studies.
Persistent Identifierhttp://hdl.handle.net/10722/314633
ISBN
9781071621639

 

DC FieldValueLanguage
dc.contributor.authorLung, SC-
dc.contributor.authorYogadasan, N-
dc.contributor.authorYanagisawa, M-
dc.contributor.authorChuong, SDX-
dc.date.accessioned2022-07-22T05:28:17Z-
dc.date.available2022-07-22T05:28:17Z-
dc.date.issued2022-
dc.identifier.citationProtoplast Isolation and Transfection in the Single-Cell C4 Species Bienertia sinuspersici. In Wang, K., Zhang, F. (Eds.), Protoplast Technology. Methods in Molecular Biology, v. 2464, p. 21-28. New York, NY: Humana, 2022-
dc.identifier.isbn9781071621639-
dc.identifier.urihttp://hdl.handle.net/10722/314633-
dc.description.abstractWe have developed an optimized protocol for isolating protoplasts from chlorenchyma cells of the single-cell C4 species Bienertia sinuspersici. The isolated protoplasts maintained the integrity of the unique single-cell C4 intracellular compartmentation of organelles as observed in chlorenchyma cells after cell wall digestion. Approximately over 80% of isolated protoplasts expressed the fusion reporter gene following the polyethylene glycol-mediated transfection procedures. Overall, fluorescent protein fusion tagged with various intraorganellular sorting signals validated the potential use of the transient gene expression system in subcellular localization and organelle dynamics studies.-
dc.languageeng-
dc.publisherHumana-
dc.relation.ispartofProtoplast Technology. Methods in Molecular Biology-
dc.titleProtoplast Isolation and Transfection in the Single-Cell C4 Species Bienertia sinuspersici-
dc.typeBook_Chapter-
dc.identifier.emailLung, SC: sclung@hku.hk-
dc.identifier.authorityLung, SC=rp02918-
dc.identifier.doi10.1007/978-1-0716-2164-6_2-
dc.identifier.hkuros334412-
dc.identifier.volume2464-
dc.identifier.spage21-
dc.identifier.epage28-
dc.publisher.placeNew York, NY-

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