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- Publisher Website: 10.1016/j.aquatox.2007.02.010
- Scopus: eid_2-s2.0-34047222398
- PMID: 17383024
- WOS: WOS:000246138800004
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Article: Induction and recovery of morphofunctional changes in the intestine of juvenile carnivorous fish (Epinephelus coioides) upon exposure to foodborne benzo[a]pyrene
Title | Induction and recovery of morphofunctional changes in the intestine of juvenile carnivorous fish (Epinephelus coioides) upon exposure to foodborne benzo[a]pyrene |
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Authors | |
Keywords | Dietary B[a]P Histopathology Immunohistochemistry Intestine Recovery |
Issue Date | 2007 |
Citation | Aquatic Toxicology, 2007, v. 82, n. 3, p. 181-194 How to Cite? |
Abstract | The sublethal toxicity of dietary benzo[a]pyrene, B[a]P, on fish growth and intestinal morphofunctional changes [as measured by epithelial turnover, cell proliferation, hyperplasia, de novo crypt formation and protein absorption efficiency (i.e. expression of proton/peptide co-transporter, PepT-1, on the mucosal brush border)] were studied for the carnivorous orange-spotted grouper (Epinephelus coioides). Juvenile fish were force-fed daily with pellets containing environmentally realistic concentrations of B[a]P (dissolved in corn oil) at 0.25 μg/g body weight (low-dose) and 12.5 μg/g body weight (high-dose) for 4 weeks, followed by a control diet for a further 4 weeks to assess recovery. Although growth inhibition was observed in fish treated with high-dose B[a]P during the exposure period, no mortality was observed throughout the 8-week experiment. Significant hyperplasia of basal enterocytes of mucosal folds was detected shortly after 3-day exposure to the high-dose B[a]P. Moreover, a faster epithelial turnover was measured in the high-dose B[a]P exposed fish at exposure week 1, which was followed by an increase of basal cell proliferation and a reduction of PepT-1 expression at exposure week 2. The formation of de novo crypts, resemblance to the cancer predisposition syndrome "juvenile polyposis", was significantly higher in the intestine of high-dose treated fish as compared to the control at exposure week 2 and onwards. Abnormal cytoplasmic extrusions were frequently observed in mucosal folds of high-dose fish at exposure week 4. In the low-dose treatment group, only the expression of PepT-1 was significantly reduced at exposure week 2 and an early adaptive response was observed at exposure week 4. Despite all these intestinal disturbances were reversible in fish upon the abatement to dietary B[a]P (within 1-4 weeks), environmental realistic levels of foodborne B[a]P could induce sublethal toxicity to E. coioides, and probably impose potential risk to the marine environment. As an increase in de novo crypts was observed towards the end of the 4-week depuration period, the long-term impacts of dietary B[a]P on fish intestinal neoplasm formation worth further investigation. © 2007 Elsevier B.V. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/313022 |
ISSN | 2023 Impact Factor: 4.1 2023 SCImago Journal Rankings: 1.099 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Yuen, Bonny B.H. | - |
dc.contributor.author | Wong, Chris K.C. | - |
dc.contributor.author | Woo, N. Y.S. | - |
dc.contributor.author | Au, Doris W.T. | - |
dc.date.accessioned | 2022-05-26T07:00:06Z | - |
dc.date.available | 2022-05-26T07:00:06Z | - |
dc.date.issued | 2007 | - |
dc.identifier.citation | Aquatic Toxicology, 2007, v. 82, n. 3, p. 181-194 | - |
dc.identifier.issn | 0166-445X | - |
dc.identifier.uri | http://hdl.handle.net/10722/313022 | - |
dc.description.abstract | The sublethal toxicity of dietary benzo[a]pyrene, B[a]P, on fish growth and intestinal morphofunctional changes [as measured by epithelial turnover, cell proliferation, hyperplasia, de novo crypt formation and protein absorption efficiency (i.e. expression of proton/peptide co-transporter, PepT-1, on the mucosal brush border)] were studied for the carnivorous orange-spotted grouper (Epinephelus coioides). Juvenile fish were force-fed daily with pellets containing environmentally realistic concentrations of B[a]P (dissolved in corn oil) at 0.25 μg/g body weight (low-dose) and 12.5 μg/g body weight (high-dose) for 4 weeks, followed by a control diet for a further 4 weeks to assess recovery. Although growth inhibition was observed in fish treated with high-dose B[a]P during the exposure period, no mortality was observed throughout the 8-week experiment. Significant hyperplasia of basal enterocytes of mucosal folds was detected shortly after 3-day exposure to the high-dose B[a]P. Moreover, a faster epithelial turnover was measured in the high-dose B[a]P exposed fish at exposure week 1, which was followed by an increase of basal cell proliferation and a reduction of PepT-1 expression at exposure week 2. The formation of de novo crypts, resemblance to the cancer predisposition syndrome "juvenile polyposis", was significantly higher in the intestine of high-dose treated fish as compared to the control at exposure week 2 and onwards. Abnormal cytoplasmic extrusions were frequently observed in mucosal folds of high-dose fish at exposure week 4. In the low-dose treatment group, only the expression of PepT-1 was significantly reduced at exposure week 2 and an early adaptive response was observed at exposure week 4. Despite all these intestinal disturbances were reversible in fish upon the abatement to dietary B[a]P (within 1-4 weeks), environmental realistic levels of foodborne B[a]P could induce sublethal toxicity to E. coioides, and probably impose potential risk to the marine environment. As an increase in de novo crypts was observed towards the end of the 4-week depuration period, the long-term impacts of dietary B[a]P on fish intestinal neoplasm formation worth further investigation. © 2007 Elsevier B.V. All rights reserved. | - |
dc.language | eng | - |
dc.relation.ispartof | Aquatic Toxicology | - |
dc.subject | Dietary B[a]P | - |
dc.subject | Histopathology | - |
dc.subject | Immunohistochemistry | - |
dc.subject | Intestine | - |
dc.subject | Recovery | - |
dc.title | Induction and recovery of morphofunctional changes in the intestine of juvenile carnivorous fish (Epinephelus coioides) upon exposure to foodborne benzo[a]pyrene | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.aquatox.2007.02.010 | - |
dc.identifier.pmid | 17383024 | - |
dc.identifier.scopus | eid_2-s2.0-34047222398 | - |
dc.identifier.volume | 82 | - |
dc.identifier.issue | 3 | - |
dc.identifier.spage | 181 | - |
dc.identifier.epage | 194 | - |
dc.identifier.isi | WOS:000246138800004 | - |