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Article: A single dose of whole inactivated H7N9 influenza vaccine confers protection from severe disease but not infection in ferrets

TitleA single dose of whole inactivated H7N9 influenza vaccine confers protection from severe disease but not infection in ferrets
Authors
KeywordsFerrets
H7N9
Vaccine
Issue Date2014
Citation
Vaccine, 2014, v. 32, n. 35, p. 4571-4577 How to Cite?
AbstractThe H7N9 influenza virus caused significant mortality and morbidity in infected humans during an outbreak in China in 2013 stimulating vaccine development efforts. As previous H7-based vaccines have been poorly immunogenic in humans we sought to determine the immunogenic and protective properties of an inactivated whole virus vaccine derived from a 2013 H7N9 virus in ferrets. As whole virus vaccine preparations have been shown to be more immunogenic in humans, but less likely to be used, than split or surface antigen formulations, we vaccinated ferrets with a single dose of 15, 30, or 50 μg of the vaccine and subsequently challenged with wild-type A/Anhui/1/2013 (H7N9) either by direct instillation or by contact with infected animals. Although ferrets vaccinated with higher doses of vaccine had higher serum hemagglutinin inhibition (HI) titers, the titers were still low. During subsequent instillation challenge, however, ferrets vaccinated with 50 μg of vaccine showed no illness and shed significantly less virus than mock vaccinated controls. All vaccinated ferrets had lower virus loads in their lungs as compared to controls. In a separate study where unvaccinated-infected ferrets were placed in the same cage with vaccinated-uninfected ferrets, vaccination did not prevent infection in the contact ferrets, although they showed a trend of lower viral load. Overall, we conclude that inactivated whole-virus H7N9 vaccine was able to reduce the severity of infection and viral load, despite the lack of hemagglutinin-inhibiting antibodies. © 2014 Elsevier Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/311967
ISSN
2023 Impact Factor: 4.5
2023 SCImago Journal Rankings: 1.342
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWong, Sook San-
dc.contributor.authorJeevan, Trushar-
dc.contributor.authorKercher, Lisa-
dc.contributor.authorYoon, Sun Woo-
dc.contributor.authorPetkova, Atanaska Marinova-
dc.contributor.authorCrumpton, Jeri Carol-
dc.contributor.authorFranks, John-
dc.contributor.authorDebeauchamp, Jennifer-
dc.contributor.authorRubrum, Adam-
dc.contributor.authorSeiler, Patrick-
dc.contributor.authorKrauss, Scott-
dc.contributor.authorWebster, Robert-
dc.contributor.authorWebby, Richard J.-
dc.date.accessioned2022-04-06T04:31:52Z-
dc.date.available2022-04-06T04:31:52Z-
dc.date.issued2014-
dc.identifier.citationVaccine, 2014, v. 32, n. 35, p. 4571-4577-
dc.identifier.issn0264-410X-
dc.identifier.urihttp://hdl.handle.net/10722/311967-
dc.description.abstractThe H7N9 influenza virus caused significant mortality and morbidity in infected humans during an outbreak in China in 2013 stimulating vaccine development efforts. As previous H7-based vaccines have been poorly immunogenic in humans we sought to determine the immunogenic and protective properties of an inactivated whole virus vaccine derived from a 2013 H7N9 virus in ferrets. As whole virus vaccine preparations have been shown to be more immunogenic in humans, but less likely to be used, than split or surface antigen formulations, we vaccinated ferrets with a single dose of 15, 30, or 50 μg of the vaccine and subsequently challenged with wild-type A/Anhui/1/2013 (H7N9) either by direct instillation or by contact with infected animals. Although ferrets vaccinated with higher doses of vaccine had higher serum hemagglutinin inhibition (HI) titers, the titers were still low. During subsequent instillation challenge, however, ferrets vaccinated with 50 μg of vaccine showed no illness and shed significantly less virus than mock vaccinated controls. All vaccinated ferrets had lower virus loads in their lungs as compared to controls. In a separate study where unvaccinated-infected ferrets were placed in the same cage with vaccinated-uninfected ferrets, vaccination did not prevent infection in the contact ferrets, although they showed a trend of lower viral load. Overall, we conclude that inactivated whole-virus H7N9 vaccine was able to reduce the severity of infection and viral load, despite the lack of hemagglutinin-inhibiting antibodies. © 2014 Elsevier Ltd.-
dc.languageeng-
dc.relation.ispartofVaccine-
dc.subjectFerrets-
dc.subjectH7N9-
dc.subjectVaccine-
dc.titleA single dose of whole inactivated H7N9 influenza vaccine confers protection from severe disease but not infection in ferrets-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.vaccine.2014.06.016-
dc.identifier.pmid24950355-
dc.identifier.scopuseid_2-s2.0-84904393681-
dc.identifier.volume32-
dc.identifier.issue35-
dc.identifier.spage4571-
dc.identifier.epage4577-
dc.identifier.eissn1873-2518-
dc.identifier.isiWOS:000340141200030-

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