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Article: The Spatiotemporal Limits of Developmental Erk Signaling

TitleThe Spatiotemporal Limits of Developmental Erk Signaling
Authors
KeywordsDrosophila
embryogenesis
MAP kinase
optogenetics
signal transduction
Issue Date2017
Citation
Developmental Cell, 2017, v. 40, n. 2, p. 185-192 How to Cite?
AbstractAnimal development is characterized by signaling events that occur at precise locations and times within the embryo, but determining when and where such precision is needed for proper embryogenesis has been a long-standing challenge. Here we address this question for extracellular signal regulated kinase (Erk) signaling, a key developmental patterning cue. We describe an optogenetic system for activating Erk with high spatiotemporal precision in vivo. Implementing this system in Drosophila, we find that embryogenesis is remarkably robust to ectopic Erk signaling, except from 1 to 4 hr post-fertilization, when perturbing the spatial extent of Erk pathway activation leads to dramatic disruptions of patterning and morphogenesis. Later in development, the effects of ectopic signaling are buffered, at least in part, by combinatorial mechanisms. Our approach can be used to systematically probe the differential contributions of the Ras/Erk pathway and concurrent signals, leading to a more quantitative understanding of developmental signaling.
Persistent Identifierhttp://hdl.handle.net/10722/311424
ISSN
2023 Impact Factor: 10.7
2023 SCImago Journal Rankings: 5.828
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorJohnson, Heath E.-
dc.contributor.authorGoyal, Yogesh-
dc.contributor.authorPannucci, Nicole L.-
dc.contributor.authorSchüpbach, Trudi-
dc.contributor.authorShvartsman, Stanislav Y.-
dc.contributor.authorToettcher, Jared E.-
dc.date.accessioned2022-03-22T11:53:54Z-
dc.date.available2022-03-22T11:53:54Z-
dc.date.issued2017-
dc.identifier.citationDevelopmental Cell, 2017, v. 40, n. 2, p. 185-192-
dc.identifier.issn1534-5807-
dc.identifier.urihttp://hdl.handle.net/10722/311424-
dc.description.abstractAnimal development is characterized by signaling events that occur at precise locations and times within the embryo, but determining when and where such precision is needed for proper embryogenesis has been a long-standing challenge. Here we address this question for extracellular signal regulated kinase (Erk) signaling, a key developmental patterning cue. We describe an optogenetic system for activating Erk with high spatiotemporal precision in vivo. Implementing this system in Drosophila, we find that embryogenesis is remarkably robust to ectopic Erk signaling, except from 1 to 4 hr post-fertilization, when perturbing the spatial extent of Erk pathway activation leads to dramatic disruptions of patterning and morphogenesis. Later in development, the effects of ectopic signaling are buffered, at least in part, by combinatorial mechanisms. Our approach can be used to systematically probe the differential contributions of the Ras/Erk pathway and concurrent signals, leading to a more quantitative understanding of developmental signaling.-
dc.languageeng-
dc.relation.ispartofDevelopmental Cell-
dc.subjectDrosophila-
dc.subjectembryogenesis-
dc.subjectMAP kinase-
dc.subjectoptogenetics-
dc.subjectsignal transduction-
dc.titleThe Spatiotemporal Limits of Developmental Erk Signaling-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1016/j.devcel.2016.12.002-
dc.identifier.pmid28118601-
dc.identifier.pmcidPMC5289754-
dc.identifier.scopuseid_2-s2.0-85010028317-
dc.identifier.volume40-
dc.identifier.issue2-
dc.identifier.spage185-
dc.identifier.epage192-
dc.identifier.eissn1878-1551-
dc.identifier.isiWOS:000392899700009-
dc.identifier.f1000727237085-

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