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- Publisher Website: 10.1101/pdb.prot074559
- Scopus: eid_2-s2.0-84943264649
- PMID: 26430249
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Article: Isolation of chloroplasts from plant protoplasts
Title | Isolation of chloroplasts from plant protoplasts |
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Authors | |
Issue Date | 2015 |
Citation | Cold Spring Harbor Protocols, 2015, v. 2015, n. 10, p. 895-899 How to Cite? |
Abstract | Chloroplasts can be isolated from higher plants directly following homogenization; however, the resulting yield, purity, and intactness are often low, necessitating a large amount of starting material. This protocol is optimized to produce a high yield of pure chloroplasts from isolated Arabidopsis protoplasts. The two-part method is a simple, scaled-down, and low-cost procedure that readily provides healthy mesophyll protoplasts, which are then ruptured to release intact chloroplasts. Chloroplasts isolated using this method are competent for use in biochemical, cellular, and molecular analyses. |
Persistent Identifier | http://hdl.handle.net/10722/311401 |
ISSN | 2023 SCImago Journal Rankings: 0.401 |
DC Field | Value | Language |
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dc.contributor.author | Lung, Shiu Cheung | - |
dc.contributor.author | Smith, Matthew D. | - |
dc.contributor.author | Chuong, Simon D.X. | - |
dc.date.accessioned | 2022-03-22T11:53:51Z | - |
dc.date.available | 2022-03-22T11:53:51Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Cold Spring Harbor Protocols, 2015, v. 2015, n. 10, p. 895-899 | - |
dc.identifier.issn | 1940-3402 | - |
dc.identifier.uri | http://hdl.handle.net/10722/311401 | - |
dc.description.abstract | Chloroplasts can be isolated from higher plants directly following homogenization; however, the resulting yield, purity, and intactness are often low, necessitating a large amount of starting material. This protocol is optimized to produce a high yield of pure chloroplasts from isolated Arabidopsis protoplasts. The two-part method is a simple, scaled-down, and low-cost procedure that readily provides healthy mesophyll protoplasts, which are then ruptured to release intact chloroplasts. Chloroplasts isolated using this method are competent for use in biochemical, cellular, and molecular analyses. | - |
dc.language | eng | - |
dc.relation.ispartof | Cold Spring Harbor Protocols | - |
dc.title | Isolation of chloroplasts from plant protoplasts | - |
dc.type | Article | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1101/pdb.prot074559 | - |
dc.identifier.pmid | 26430249 | - |
dc.identifier.scopus | eid_2-s2.0-84943264649 | - |
dc.identifier.volume | 2015 | - |
dc.identifier.issue | 10 | - |
dc.identifier.spage | 895 | - |
dc.identifier.epage | 899 | - |
dc.identifier.eissn | 1559-6095 | - |