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- Publisher Website: 10.1016/j.devcel.2014.10.026
- Scopus: eid_2-s2.0-84922365403
- PMID: 25543281
- WOS: WOS:000347907200009
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Article: Profilin-1 Serves as a gatekeeper for actin assembly by Arp2/3-Dependent and -Independent pathways
Title | Profilin-1 Serves as a gatekeeper for actin assembly by Arp2/3-Dependent and -Independent pathways |
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Authors | |
Issue Date | 2015 |
Citation | Developmental Cell, 2015, v. 32, n. 1, p. 54-67 How to Cite? |
Abstract | Cells contain multiple F-actin assembly pathways, including the Arp2/3 complex, formins, and Ena/VASP, which have largely been analyzed separately. They collectively generate the bulk of F-actin from a common pool of G-actin; however, the interplay and/or competition between these pathways remains poorly understood. Using fibroblast lines derived from an Arpc2 conditional knockout mouse, we established matched-pair cells with and without the Arp2/3 complex. Arpc2-/- cells lack lamellipodia and migrate more slowly than WT cells but have F-actin levels indistinguishable from controls. Actin assembly in Arpc2-/- cells was resistant to cytochalasin-D and was highly dependent on profilin-1 and Ena/VASP but not formins. Profilin-1 depletion in WT cells increased F-actin and Arp2/3 complex in lamellipodia. Conversely, addition of exogenous profilin-1 inhibited Arp2/3 complex actin nucleation invitro and invivo. Antagonism of the Arp2/3 complex by profilin-1 in cells appears to maintain actin homeostasis by balancing Arp2/3 complex-dependent and-independent actin assembly pathways. |
Persistent Identifier | http://hdl.handle.net/10722/311391 |
ISSN | 2023 Impact Factor: 10.7 2023 SCImago Journal Rankings: 5.828 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Rotty, Jeremy D. | - |
dc.contributor.author | Wu, Congying | - |
dc.contributor.author | Haynes, Elizabeth M. | - |
dc.contributor.author | Suarez, Cristian | - |
dc.contributor.author | Winkelman, Jonathan D. | - |
dc.contributor.author | Johnson, Heath E. | - |
dc.contributor.author | Haugh, Jason M. | - |
dc.contributor.author | Kovar, David R. | - |
dc.contributor.author | Bear, James E. | - |
dc.date.accessioned | 2022-03-22T11:53:49Z | - |
dc.date.available | 2022-03-22T11:53:49Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Developmental Cell, 2015, v. 32, n. 1, p. 54-67 | - |
dc.identifier.issn | 1534-5807 | - |
dc.identifier.uri | http://hdl.handle.net/10722/311391 | - |
dc.description.abstract | Cells contain multiple F-actin assembly pathways, including the Arp2/3 complex, formins, and Ena/VASP, which have largely been analyzed separately. They collectively generate the bulk of F-actin from a common pool of G-actin; however, the interplay and/or competition between these pathways remains poorly understood. Using fibroblast lines derived from an Arpc2 conditional knockout mouse, we established matched-pair cells with and without the Arp2/3 complex. Arpc2-/- cells lack lamellipodia and migrate more slowly than WT cells but have F-actin levels indistinguishable from controls. Actin assembly in Arpc2-/- cells was resistant to cytochalasin-D and was highly dependent on profilin-1 and Ena/VASP but not formins. Profilin-1 depletion in WT cells increased F-actin and Arp2/3 complex in lamellipodia. Conversely, addition of exogenous profilin-1 inhibited Arp2/3 complex actin nucleation invitro and invivo. Antagonism of the Arp2/3 complex by profilin-1 in cells appears to maintain actin homeostasis by balancing Arp2/3 complex-dependent and-independent actin assembly pathways. | - |
dc.language | eng | - |
dc.relation.ispartof | Developmental Cell | - |
dc.title | Profilin-1 Serves as a gatekeeper for actin assembly by Arp2/3-Dependent and -Independent pathways | - |
dc.type | Article | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1016/j.devcel.2014.10.026 | - |
dc.identifier.pmid | 25543281 | - |
dc.identifier.pmcid | PMC4296256 | - |
dc.identifier.scopus | eid_2-s2.0-84922365403 | - |
dc.identifier.volume | 32 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | 54 | - |
dc.identifier.epage | 67 | - |
dc.identifier.eissn | 1878-1551 | - |
dc.identifier.isi | WOS:000347907200009 | - |
dc.identifier.f1000 | 725289786 | - |