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Article: Isolation of dimorphic chloroplasts from the single-cell C4 species Bienertia sinuspersici

TitleIsolation of dimorphic chloroplasts from the single-cell C<inf>4 </inf>species Bienertia sinuspersici
Authors
Lung, Shiu CheungYanagisawa, MakotoChuong, Simon D.X.
KeywordsBienertia sinuspersici
Chloroplast isolation
Dimorphic chloroplasts
Osmotic swelling
Photosynthesis
Protoplast
Single-cell C4
Vacuole isolation
Issue Date2012
Citation
Plant Methods, 2012, v. 8, n. 1, article no. 8 How to Cite?
DOI: http://dx.doi.org/10.1186/1746-4811-8-8
AbstractThree terrestrial plants are known to perform C 4 photosynthesis without the dual-cell system by partitioning two distinct types of chloroplasts in separate cytoplasmic compartments. We report herein a protocol for isolating the dimorphic chloroplasts from Bienertia sinuspersici. Hypo-osmotically lysed protoplasts under our defined conditions released intact compartments containing the central chloroplasts and intact vacuoles with adhering peripheral chloroplasts. Following Percoll step gradient purification both chloroplast preparations demonstrated high homogeneities as evaluated from the relative abundance of respective protein markers. This protocol will open novel research directions toward understanding the mechanism of single-cell C 4 photosynthesis. © 2012 Lung et al; licensee BioMed Central Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/311370
PubMed Central ID
PMC3310801
ISI Accession Number ID
WOS:000302233600001

 

DC FieldValueLanguage
dc.contributor.authorLung, Shiu Cheung-
dc.contributor.authorYanagisawa, Makoto-
dc.contributor.authorChuong, Simon D.X.-
dc.date.accessioned2022-03-22T11:53:46Z-
dc.date.available2022-03-22T11:53:46Z-
dc.date.issued2012-
dc.identifier.citationPlant Methods, 2012, v. 8, n. 1, article no. 8-
dc.identifier.urihttp://hdl.handle.net/10722/311370-
dc.description.abstractThree terrestrial plants are known to perform C 4 photosynthesis without the dual-cell system by partitioning two distinct types of chloroplasts in separate cytoplasmic compartments. We report herein a protocol for isolating the dimorphic chloroplasts from Bienertia sinuspersici. Hypo-osmotically lysed protoplasts under our defined conditions released intact compartments containing the central chloroplasts and intact vacuoles with adhering peripheral chloroplasts. Following Percoll step gradient purification both chloroplast preparations demonstrated high homogeneities as evaluated from the relative abundance of respective protein markers. This protocol will open novel research directions toward understanding the mechanism of single-cell C 4 photosynthesis. © 2012 Lung et al; licensee BioMed Central Ltd.-
dc.languageeng-
dc.relation.ispartofPlant Methods-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectBienertia sinuspersici-
dc.subjectChloroplast isolation-
dc.subjectDimorphic chloroplasts-
dc.subjectOsmotic swelling-
dc.subjectPhotosynthesis-
dc.subjectProtoplast-
dc.subjectSingle-cell C4-
dc.subjectVacuole isolation-
dc.titleIsolation of dimorphic chloroplasts from the single-cell C<inf>4 </inf>species Bienertia sinuspersici-
dc.typeArticle-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1186/1746-4811-8-8-
dc.identifier.pmid22394490-
dc.identifier.pmcidPMC3310801-
dc.identifier.scopuseid_2-s2.0-84857734008-
dc.identifier.volume8-
dc.identifier.issue1-
dc.identifier.spagearticle no. 8-
dc.identifier.epagearticle no. 8-
dc.identifier.eissn1746-4811-
dc.identifier.isiWOS:000302233600001-

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