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- Publisher Website: 10.1016/j.brainresbull.2021.02.016
- Scopus: eid_2-s2.0-85101811284
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Article: Promotion of Momordica Charantia polysaccharides on neural stem cell proliferation by increasing SIRT1 activity after cerebral ischemia/reperfusion in rats
Title | Promotion of Momordica Charantia polysaccharides on neural stem cell proliferation by increasing SIRT1 activity after cerebral ischemia/reperfusion in rats |
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Authors | |
Keywords | Momordica charantia polysaccharides (MCPs) Neural stem cells (NSCs) Proliferation SIRT1 β-catenin |
Issue Date | 2021 |
Publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/brainresbull |
Citation | Brain Research Bulletin, 2021, v. 170, p. 254-263 How to Cite? |
Abstract | The deacetylase SIRT1 has been reported to play a critical role in regulating neurogenesis, which may be an adaptive processes contributing to recovery after stroke. Our previous work showed that the antioxidant capacity of Momordica charantia polysaccharides (MCPs) could protect against cerebral ischemia/reperfusion (I/R) after stroke. However, whether the protective effect of MCPs on I/R injury is related to neural stem cell (NSC) proliferation remains unclear. In the present study, we designed invivo and invitro experiments to elucidate the underlying mechanisms by which MCPs promote endogenous NSC proliferation during cerebral I/R. Invivo results showed that MCPs rescued the memory and learning abilities of rats after I/R damage and enhanced NSC proliferation in the rat subventricular zone (SVZ) and subgrannular zone (SGZ) during I/R. Invitro experiments demonstrated that MCPs could stimulate the proliferation of C17.2 cells under oxygen-glucose deprivation (OGD) conditions. Further studies revealed that the proliferation-promoting mechanism of MCPs relied on increasing the activity of SIRT1, decreasing the level of acetylation of β-catenin in the cytoplasm, and then triggering the translocation of β-catenin into the nucleus. These data provide experimental evidence that the up-regulation of SIRT1 activity by MCPs led to an increased cytoplasmic deacetylation of β-catenin, which promoted translocation of β-catenin to the nucleus to participate in the signaling pathway involved in NSC proliferation. The present study reveals that MCPs function as a therapeutic drug to promote stroke recovery by increasing the activity of SIRT1, decreasing the level of acetylated β-catenin, promoting the nuclear translocation of β-catenin and thereby increasing endogenous NSC proliferation. |
Persistent Identifier | http://hdl.handle.net/10722/310489 |
ISSN | 2021 Impact Factor: 3.715 2020 SCImago Journal Rankings: 1.097 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Ma, J | - |
dc.contributor.author | Fan, H | - |
dc.contributor.author | Cai, Heng | - |
dc.contributor.author | Hu, Z | - |
dc.contributor.author | Zhou, X | - |
dc.contributor.author | Li, F | - |
dc.contributor.author | Chen, H | - |
dc.contributor.author | Shen, J | - |
dc.contributor.author | Qi, S | - |
dc.date.accessioned | 2022-02-07T07:57:24Z | - |
dc.date.available | 2022-02-07T07:57:24Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Brain Research Bulletin, 2021, v. 170, p. 254-263 | - |
dc.identifier.issn | 0361-9230 | - |
dc.identifier.uri | http://hdl.handle.net/10722/310489 | - |
dc.description.abstract | The deacetylase SIRT1 has been reported to play a critical role in regulating neurogenesis, which may be an adaptive processes contributing to recovery after stroke. Our previous work showed that the antioxidant capacity of Momordica charantia polysaccharides (MCPs) could protect against cerebral ischemia/reperfusion (I/R) after stroke. However, whether the protective effect of MCPs on I/R injury is related to neural stem cell (NSC) proliferation remains unclear. In the present study, we designed invivo and invitro experiments to elucidate the underlying mechanisms by which MCPs promote endogenous NSC proliferation during cerebral I/R. Invivo results showed that MCPs rescued the memory and learning abilities of rats after I/R damage and enhanced NSC proliferation in the rat subventricular zone (SVZ) and subgrannular zone (SGZ) during I/R. Invitro experiments demonstrated that MCPs could stimulate the proliferation of C17.2 cells under oxygen-glucose deprivation (OGD) conditions. Further studies revealed that the proliferation-promoting mechanism of MCPs relied on increasing the activity of SIRT1, decreasing the level of acetylation of β-catenin in the cytoplasm, and then triggering the translocation of β-catenin into the nucleus. These data provide experimental evidence that the up-regulation of SIRT1 activity by MCPs led to an increased cytoplasmic deacetylation of β-catenin, which promoted translocation of β-catenin to the nucleus to participate in the signaling pathway involved in NSC proliferation. The present study reveals that MCPs function as a therapeutic drug to promote stroke recovery by increasing the activity of SIRT1, decreasing the level of acetylated β-catenin, promoting the nuclear translocation of β-catenin and thereby increasing endogenous NSC proliferation. | - |
dc.language | eng | - |
dc.publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/brainresbull | - |
dc.relation.ispartof | Brain Research Bulletin | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | Momordica charantia polysaccharides (MCPs) | - |
dc.subject | Neural stem cells (NSCs) | - |
dc.subject | Proliferation | - |
dc.subject | SIRT1 | - |
dc.subject | β-catenin | - |
dc.title | Promotion of Momordica Charantia polysaccharides on neural stem cell proliferation by increasing SIRT1 activity after cerebral ischemia/reperfusion in rats | - |
dc.type | Article | - |
dc.identifier.email | Shen, J: shenjg@hku.hk | - |
dc.identifier.authority | Shen, J=rp00487 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1016/j.brainresbull.2021.02.016 | - |
dc.identifier.pmid | 33647420 | - |
dc.identifier.scopus | eid_2-s2.0-85101811284 | - |
dc.identifier.hkuros | 331615 | - |
dc.identifier.volume | 170 | - |
dc.identifier.spage | 254 | - |
dc.identifier.epage | 263 | - |
dc.identifier.isi | WOS:000631180800028 | - |
dc.publisher.place | United States | - |