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Article: Genomic investigation of a Streptococcus pneumoniae serotype 24F strain causing meningoencephalitis in Hong Kong

TitleGenomic investigation of a Streptococcus pneumoniae serotype 24F strain causing meningoencephalitis in Hong Kong
Authors
KeywordsPneumococcal conjugate vaccine
Epidemiology
Invasive pneumococcal disease
Cotrimoxazole resistance
Issue Date2021
PublisherUrban und Fischer Verlag. The Journal's web site is located at http://www.elsevier.com/locate/ijmm
Citation
International Journal of Medical Microbiology, 2021, v. 311 n. 8, article no. 151543 How to Cite?
AbstractPneumococcal conjugate vaccines (PCVs) successfully decreased the incidence of invasive pneumococcal disease in children. However, many countries have reported serotype replacement and a rebound in diseases from non-vaccine serotypes. Here, we report the genomic investigation of a Streptococcus pneumoniae strain M215 that caused severe meningoencephalitis in an infant in 2019. The strain was assigned to serotype 24F using the bioinformatic pipeline SeroBA and pneumococcal type specific anti-sera. The strain was resistant to cotrimoxazole from mutations in both folA and folP genes. It was susceptible to penicillin and other non-β-lactam antibiotics. Phylogenetically, it belongs to Global Pneumococcal Sequence Cluster (GPSC) 6 and multi-locus sequence type 162. A total of 38 virulence genes were detected in the genome of M215. Upon comparison of the profile of virulence genes, GPSC6 but not non-GPSC6 strains of serotype 24F and related serotypes were found to possess the major virulence determinant, pilus islet-1, comprising genes encoding sortases (srtB, srtC, srtD), pilus proteins (rrgA, rrgB and rrgC) and one transcriptional regulator (rlrA), which was previously described to be characteristic feature of international clones in the pre-PCV era. In our locality, this represented the first detection of serotype 24F and GPSC6/ST162 causing serious pneumococcal disease. The emergence of the non-vaccine serotype 24F GPSC6/ST162 lineage with molecular feature of high virulence is concerning and emphasizes the need for full characterization of strains causing severe disease.
Persistent Identifierhttp://hdl.handle.net/10722/309104
ISSN
2023 Impact Factor: 4.5
2023 SCImago Journal Rankings: 1.196
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorCao, H-
dc.contributor.authorChiu, KHY-
dc.contributor.authorChiu, SS-
dc.contributor.authorJiang, S-
dc.contributor.authorChow, KH-
dc.contributor.authorHo, PL-
dc.date.accessioned2021-12-14T01:40:37Z-
dc.date.available2021-12-14T01:40:37Z-
dc.date.issued2021-
dc.identifier.citationInternational Journal of Medical Microbiology, 2021, v. 311 n. 8, article no. 151543-
dc.identifier.issn1438-4221-
dc.identifier.urihttp://hdl.handle.net/10722/309104-
dc.description.abstractPneumococcal conjugate vaccines (PCVs) successfully decreased the incidence of invasive pneumococcal disease in children. However, many countries have reported serotype replacement and a rebound in diseases from non-vaccine serotypes. Here, we report the genomic investigation of a Streptococcus pneumoniae strain M215 that caused severe meningoencephalitis in an infant in 2019. The strain was assigned to serotype 24F using the bioinformatic pipeline SeroBA and pneumococcal type specific anti-sera. The strain was resistant to cotrimoxazole from mutations in both folA and folP genes. It was susceptible to penicillin and other non-β-lactam antibiotics. Phylogenetically, it belongs to Global Pneumococcal Sequence Cluster (GPSC) 6 and multi-locus sequence type 162. A total of 38 virulence genes were detected in the genome of M215. Upon comparison of the profile of virulence genes, GPSC6 but not non-GPSC6 strains of serotype 24F and related serotypes were found to possess the major virulence determinant, pilus islet-1, comprising genes encoding sortases (srtB, srtC, srtD), pilus proteins (rrgA, rrgB and rrgC) and one transcriptional regulator (rlrA), which was previously described to be characteristic feature of international clones in the pre-PCV era. In our locality, this represented the first detection of serotype 24F and GPSC6/ST162 causing serious pneumococcal disease. The emergence of the non-vaccine serotype 24F GPSC6/ST162 lineage with molecular feature of high virulence is concerning and emphasizes the need for full characterization of strains causing severe disease.-
dc.languageeng-
dc.publisherUrban und Fischer Verlag. The Journal's web site is located at http://www.elsevier.com/locate/ijmm-
dc.relation.ispartofInternational Journal of Medical Microbiology-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectPneumococcal conjugate vaccine-
dc.subjectEpidemiology-
dc.subjectInvasive pneumococcal disease-
dc.subjectCotrimoxazole resistance-
dc.titleGenomic investigation of a Streptococcus pneumoniae serotype 24F strain causing meningoencephalitis in Hong Kong-
dc.typeArticle-
dc.identifier.emailCao, H: hcao@hku.hk-
dc.identifier.emailChiu, SS: ssschiu@hku.hk-
dc.identifier.emailChow, KH: khchowb@hku.hk-
dc.identifier.emailHo, PL: plho@hku.hk-
dc.identifier.authorityChiu, SS=rp00421-
dc.identifier.authorityChow, KH=rp00370-
dc.identifier.authorityHo, PL=rp00406-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1016/j.ijmm.2021.151543-
dc.identifier.pmid34864352-
dc.identifier.scopuseid_2-s2.0-85120857305-
dc.identifier.hkuros331013-
dc.identifier.volume311-
dc.identifier.issue8-
dc.identifier.spagearticle no. 151543-
dc.identifier.epagearticle no. 151543-
dc.identifier.isiWOS:000771515600002-
dc.publisher.placeGermany-

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