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Article: A dual-function chemical probe for detecting erasers of lysine lipoylation

TitleA dual-function chemical probe for detecting erasers of lysine lipoylation
Authors
KeywordsDual-function
Fluorescent probe
Labeling
Photo-cross-linker
Lipoylation modification
Eraser
Sirtuin
Issue Date2022
PublisherSpringer Verlag. The Journal's web site is located at http://www.springerlink.com/content/2095-0179/
Citation
Frontiers of Chemical Science and Engineering, 2022, v. 16 n. 1, p. 121-127 How to Cite?
AbstractLysine lipoylation plays vital roles in cell metabolism and redox processes. For example, removal of lipoylation will decrease pyruvate dehydrogenase activity and affect the citric acid cycle. Despite the important functions of lysine lipoylation, the mechanisms for the addition and removal of this modification remain largely unexplored. Very few useful chemical tools are available to study the interactions of lysine lipoylation with its regulatory delipoylation proteins. For example, immunoaffinity purification-mass spectrometry is one of such tools, which highly relies on antibody efficiency and purification techniques. Single-step activity based fluorogenic probes developed by our groups and others is also an efficient method to study the deacylation activity. Affinity-based labeling probe using photo-cross-linker is a powerful platform to study the transient and dynamic interactions of peptide ligands with the interacting proteins. Herein, we have designed and synthesized a dual-function probe KTLlip for studying enzymatic delipoylation (eraser) activity and interaction of lysine lipoylation with the eraser at the same time. We show that KTLlip can be used as a useful tool to detect delipoylation as demonstrated by its ability to fluorescently label the eraser activity of recombinant Sirt2. We envision that the probe will help delineate the roles of delipoylation enzyme in biology.
Persistent Identifierhttp://hdl.handle.net/10722/307822
ISSN
2023 Impact Factor: 4.3
2023 SCImago Journal Rankings: 0.792
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorXie, Y-
dc.contributor.authorZhang, J-
dc.contributor.authorYang, L-
dc.contributor.authorChen, Q-
dc.contributor.authorHao, Q-
dc.contributor.authorZhang, L-
dc.contributor.authorSun, H-
dc.date.accessioned2021-11-12T13:38:24Z-
dc.date.available2021-11-12T13:38:24Z-
dc.date.issued2022-
dc.identifier.citationFrontiers of Chemical Science and Engineering, 2022, v. 16 n. 1, p. 121-127-
dc.identifier.issn2095-0179-
dc.identifier.urihttp://hdl.handle.net/10722/307822-
dc.description.abstractLysine lipoylation plays vital roles in cell metabolism and redox processes. For example, removal of lipoylation will decrease pyruvate dehydrogenase activity and affect the citric acid cycle. Despite the important functions of lysine lipoylation, the mechanisms for the addition and removal of this modification remain largely unexplored. Very few useful chemical tools are available to study the interactions of lysine lipoylation with its regulatory delipoylation proteins. For example, immunoaffinity purification-mass spectrometry is one of such tools, which highly relies on antibody efficiency and purification techniques. Single-step activity based fluorogenic probes developed by our groups and others is also an efficient method to study the deacylation activity. Affinity-based labeling probe using photo-cross-linker is a powerful platform to study the transient and dynamic interactions of peptide ligands with the interacting proteins. Herein, we have designed and synthesized a dual-function probe KTLlip for studying enzymatic delipoylation (eraser) activity and interaction of lysine lipoylation with the eraser at the same time. We show that KTLlip can be used as a useful tool to detect delipoylation as demonstrated by its ability to fluorescently label the eraser activity of recombinant Sirt2. We envision that the probe will help delineate the roles of delipoylation enzyme in biology.-
dc.languageeng-
dc.publisherSpringer Verlag. The Journal's web site is located at http://www.springerlink.com/content/2095-0179/-
dc.relation.ispartofFrontiers of Chemical Science and Engineering-
dc.subjectDual-function-
dc.subjectFluorescent probe-
dc.subjectLabeling-
dc.subjectPhoto-cross-linker-
dc.subjectLipoylation modification-
dc.subjectEraser-
dc.subjectSirtuin-
dc.titleA dual-function chemical probe for detecting erasers of lysine lipoylation-
dc.typeArticle-
dc.identifier.emailHao, Q: qhao@hku.hk-
dc.identifier.authorityHao, Q=rp01332-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s11705-021-2051-0-
dc.identifier.scopuseid_2-s2.0-85105511295-
dc.identifier.hkuros329864-
dc.identifier.volume16-
dc.identifier.issue1-
dc.identifier.spage121-
dc.identifier.epage127-
dc.identifier.isiWOS:000645516400003-
dc.publisher.placeChina-

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