File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Proteomic analysis of circulating extracellular vesicles identifies potential biomarkers for lymph node metastasis in oral tongue squamous cell carcinoma

TitleProteomic analysis of circulating extracellular vesicles identifies potential biomarkers for lymph node metastasis in oral tongue squamous cell carcinoma
Authors
Keywordsextracellular vesicles
oral tongue squamous cell carcinoma
lymph node metastases
proteomics
biomarkers
Issue Date2021
PublisherMDPI AG. The Journal's web site is located at http://www.mdpi.com/journal/cells
Citation
Cells, 2021, v. 10 n. 9, p. article no. 2179 How to Cite?
AbstractLymph node metastasis is the most reliable indicator of a poor prognosis for patients with oral tongue cancers. Currently, there are no biomarkers to predict whether a cancer will spread in the future if it has not already spread at the time of diagnosis. The aim of this study was to quantitatively profile the proteomes of extracellular vesicles (EVs) isolated from blood samples taken from patients with oral tongue squamous cell carcinoma with and without lymph node involvement and non-cancer controls. EVs were enriched using size exclusion chromatography (SEC) from pooled plasma samples of patients with non-nodal and nodal oral tongue squamous cell carcinoma (OTSCC) and non-cancer controls. Protein cargo was quantitatively profiled using isobaric labelling (iTRAQ) and two-dimensional high-performance liquid chromatography followed by tandem mass spectrometry. We identified 208 EV associated proteins and, after filtering, generated a short list of 136 proteins. Over 85% of the EV-associated proteins were associated with the GO cellular compartment term “extracellular exosome”. Comparisons between non-cancer controls and oral tongue squamous cell carcinoma with and without lymph node involvement revealed 43 unique candidate EV-associated proteins with deregulated expression patterns. The shortlisted EV associated proteins described here may be useful discriminatory biomarkers for differentiating OTSCC with and without nodal disease or non-cancer controls.
Persistent Identifierhttp://hdl.handle.net/10722/307753
ISSN
2023 Impact Factor: 5.1
2023 SCImago Journal Rankings: 1.547
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorQu, X-
dc.contributor.authorLeung, TCN-
dc.contributor.authorNgai, SM-
dc.contributor.authorTsai, SN-
dc.contributor.authorThakur, A-
dc.contributor.authorLi, WK-
dc.contributor.authorLee, Y-
dc.contributor.authorLeung, L-
dc.contributor.authorNg, TH-
dc.contributor.authorYam, J-
dc.contributor.authorLan, L-
dc.contributor.authorLau, EHL-
dc.contributor.authorWong, EWY-
dc.contributor.authorChan, JYK-
dc.contributor.authorMeehan, K-
dc.date.accessioned2021-11-12T13:37:19Z-
dc.date.available2021-11-12T13:37:19Z-
dc.date.issued2021-
dc.identifier.citationCells, 2021, v. 10 n. 9, p. article no. 2179-
dc.identifier.issn2073-4409-
dc.identifier.urihttp://hdl.handle.net/10722/307753-
dc.description.abstractLymph node metastasis is the most reliable indicator of a poor prognosis for patients with oral tongue cancers. Currently, there are no biomarkers to predict whether a cancer will spread in the future if it has not already spread at the time of diagnosis. The aim of this study was to quantitatively profile the proteomes of extracellular vesicles (EVs) isolated from blood samples taken from patients with oral tongue squamous cell carcinoma with and without lymph node involvement and non-cancer controls. EVs were enriched using size exclusion chromatography (SEC) from pooled plasma samples of patients with non-nodal and nodal oral tongue squamous cell carcinoma (OTSCC) and non-cancer controls. Protein cargo was quantitatively profiled using isobaric labelling (iTRAQ) and two-dimensional high-performance liquid chromatography followed by tandem mass spectrometry. We identified 208 EV associated proteins and, after filtering, generated a short list of 136 proteins. Over 85% of the EV-associated proteins were associated with the GO cellular compartment term “extracellular exosome”. Comparisons between non-cancer controls and oral tongue squamous cell carcinoma with and without lymph node involvement revealed 43 unique candidate EV-associated proteins with deregulated expression patterns. The shortlisted EV associated proteins described here may be useful discriminatory biomarkers for differentiating OTSCC with and without nodal disease or non-cancer controls.-
dc.languageeng-
dc.publisherMDPI AG. The Journal's web site is located at http://www.mdpi.com/journal/cells-
dc.relation.ispartofCells-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectextracellular vesicles-
dc.subjectoral tongue squamous cell carcinoma-
dc.subjectlymph node metastases-
dc.subjectproteomics-
dc.subjectbiomarkers-
dc.titleProteomic analysis of circulating extracellular vesicles identifies potential biomarkers for lymph node metastasis in oral tongue squamous cell carcinoma-
dc.typeArticle-
dc.identifier.emailNg, TH: tonyng93@hku.hk-
dc.identifier.emailYam, J: judyyam@pathology.hku.hk-
dc.identifier.authorityYam, J=rp00468-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3390/cells10092179-
dc.identifier.pmid34571828-
dc.identifier.pmcidPMC8468562-
dc.identifier.scopuseid_2-s2.0-85115902320-
dc.identifier.hkuros329578-
dc.identifier.volume10-
dc.identifier.issue9-
dc.identifier.spagearticle no. 2179-
dc.identifier.epagearticle no. 2179-
dc.identifier.isiWOS:000699062500001-
dc.publisher.placeSwitzerland-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats