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Article: Improved Detection of mecA-Mediated β-Lactam Resistance in Staphylococcus lugdunensis Using a New Oxacillin Salt Agar Screen

TitleImproved Detection of mecA-Mediated β-Lactam Resistance in Staphylococcus lugdunensis Using a New Oxacillin Salt Agar Screen
Authors
Keywordscefoxitin
methicillin resistance and mecA gene
susceptibility test comparison
Staphylococcus lugdunensis
oxacillin agar screening method
Issue Date2021
PublisherFrontiers Research Foundation. The Journal's web site is located at http://www.frontiersin.org/microbiology/
Citation
Frontiers in Microbiology, 2021, v. 12, p. article no. 704552 How to Cite?
AbstractOxacillin resistance mediated by mecA in Staphylococcus lugdunensis is emerging in some geographic areas. We evaluated cefoxitin disk diffusion (DD) and a new oxacillin agar (supplemented with 2 μg/ml oxacillin and 2% sodium chloride) screen for the detection of mecA-mediated resistance in S. lugdunensis. A total of 300 consecutive, non-duplicated clinical S. lugdunensis isolates from diverse sources in Hong Kong in 2019 were tested. The categorical agreement and errors obtained between cefoxitin DD test, oxacillin agar screen and mecA PCR were analyzed. Isolates with discordant results were further tested by MIC, penicillin binding protein 2a (PBP2a) assays, population analysis and molecular typing. PCR showed that 62 isolates were mecA-positive and 238 isolates were mecA-negative. For cefoxitin DD results interpreted using S. aureus/S. lugdunensis breakpoints, the categorical agreement (CA) for two brands of Muller-Hinton agars, MH-II (Becton Dickinson) and MH-E (bioMérieux) were both 96.0%; MEs were both 0%; and VMEs were 19.4 and 12.9%, respectively. The new oxacillin agar reliably differentiated mecA-positive and mecA-negative isolates (100% CA) without any ME or VME results. The 8 isolates with false susceptibility in the cefoxitin DD testing had cefoxitin and oxacillin MICs in the susceptible range. The isolates showed heterogeneous oxacillin resistance with resistant subpopulations at low frequencies. All had positive PBP2a results and were typed as sequence type 27/SCCmec V. The findings highlight the inability of cefoxitin DD and MIC tests for reliable detection of some mecA-positive S. lugdunensis isolates.
Persistent Identifierhttp://hdl.handle.net/10722/307692
ISSN
2023 Impact Factor: 4.0
2023 SCImago Journal Rankings: 1.065
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHo, PL-
dc.contributor.authorLaw, YH-
dc.contributor.authorLIU, MCJ-
dc.contributor.authorLAU, A-
dc.contributor.authorTONG, MK-
dc.contributor.authorChow, KH-
dc.contributor.authorWu, AKL-
dc.contributor.authorTse, CWS-
dc.contributor.authorCheng, VCC-
dc.contributor.authorQue, TL-
dc.date.accessioned2021-11-12T13:36:25Z-
dc.date.available2021-11-12T13:36:25Z-
dc.date.issued2021-
dc.identifier.citationFrontiers in Microbiology, 2021, v. 12, p. article no. 704552-
dc.identifier.issn1664-302X-
dc.identifier.urihttp://hdl.handle.net/10722/307692-
dc.description.abstractOxacillin resistance mediated by mecA in Staphylococcus lugdunensis is emerging in some geographic areas. We evaluated cefoxitin disk diffusion (DD) and a new oxacillin agar (supplemented with 2 μg/ml oxacillin and 2% sodium chloride) screen for the detection of mecA-mediated resistance in S. lugdunensis. A total of 300 consecutive, non-duplicated clinical S. lugdunensis isolates from diverse sources in Hong Kong in 2019 were tested. The categorical agreement and errors obtained between cefoxitin DD test, oxacillin agar screen and mecA PCR were analyzed. Isolates with discordant results were further tested by MIC, penicillin binding protein 2a (PBP2a) assays, population analysis and molecular typing. PCR showed that 62 isolates were mecA-positive and 238 isolates were mecA-negative. For cefoxitin DD results interpreted using S. aureus/S. lugdunensis breakpoints, the categorical agreement (CA) for two brands of Muller-Hinton agars, MH-II (Becton Dickinson) and MH-E (bioMérieux) were both 96.0%; MEs were both 0%; and VMEs were 19.4 and 12.9%, respectively. The new oxacillin agar reliably differentiated mecA-positive and mecA-negative isolates (100% CA) without any ME or VME results. The 8 isolates with false susceptibility in the cefoxitin DD testing had cefoxitin and oxacillin MICs in the susceptible range. The isolates showed heterogeneous oxacillin resistance with resistant subpopulations at low frequencies. All had positive PBP2a results and were typed as sequence type 27/SCCmec V. The findings highlight the inability of cefoxitin DD and MIC tests for reliable detection of some mecA-positive S. lugdunensis isolates.-
dc.languageeng-
dc.publisherFrontiers Research Foundation. The Journal's web site is located at http://www.frontiersin.org/microbiology/-
dc.relation.ispartofFrontiers in Microbiology-
dc.rightsThis Document is Protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permission.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectcefoxitin-
dc.subjectmethicillin resistance and mecA gene-
dc.subjectsusceptibility test comparison-
dc.subjectStaphylococcus lugdunensis-
dc.subjectoxacillin agar screening method-
dc.titleImproved Detection of mecA-Mediated β-Lactam Resistance in Staphylococcus lugdunensis Using a New Oxacillin Salt Agar Screen-
dc.typeArticle-
dc.identifier.emailHo, PL: plho@hku.hk-
dc.identifier.emailChow, KH: khchowb@hku.hk-
dc.identifier.authorityHo, PL=rp00406-
dc.identifier.authorityChow, KH=rp00370-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3389/fmicb.2021.704552-
dc.identifier.pmid34421864-
dc.identifier.pmcidPMC8378274-
dc.identifier.scopuseid_2-s2.0-85113236390-
dc.identifier.hkuros329802-
dc.identifier.volume12-
dc.identifier.spagearticle no. 704552-
dc.identifier.epagearticle no. 704552-
dc.identifier.isiWOS:000687592700001-
dc.publisher.placeSwitzerland-

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