File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Generation of a human iPSC line GIBHi002-A-2 with a dual-reporter for NKX2-5 using TALENs

TitleGeneration of a human iPSC line GIBHi002-A-2 with a dual-reporter for NKX2-5 using TALENs
Authors
Zhou, MWei, RJiang, YFu, JLiu, YYang, BYu, BLin, YRan, XLai, WHChu, MHu, YYang, JTse, HF
Issue Date2021
PublisherElsevier BV. The Journal's web site is located at https://journals.elsevier.com/stem-cell-research
Citation
Stem Cell Research, 2021, v. 50, article no. 102120 How to Cite?
DOI: http://dx.doi.org/10.1016/j.scr.2020.102120
AbstractThe human transcription factor NKX2-5 plays an important role in cardiac formation and development, and thus it can be used for isolation of cardiomyocytes (CMs) differentiated from human pluripotent stem cells (hPSCs). Here, we knocked-in enhanced GFP (eGFP) and Pac (a puromycin resistant gene; PuroR) into the exon 1 coding region of NKX2-5 from a human iPSC line iPSN0003 using TALENs. The generated GIBHi002-A-2 enables us to monitor and optimize cardiac differentiation procedures via the cardiac progenitor cells (CPCs), as well as to isolate iPSC-derived CMs for drug screening.
Persistent Identifierhttp://hdl.handle.net/10722/307676
ISSN
1873-5061
2021 Impact Factor: 1.587
2020 SCImago Journal Rankings: 0.654
ISI Accession Number ID
WOS:000614260000012

 

DC FieldValueLanguage
dc.contributor.authorZhou, M-
dc.contributor.authorWei, R-
dc.contributor.authorJiang, Y-
dc.contributor.authorFu, J-
dc.contributor.authorLiu, Y-
dc.contributor.authorYang, B-
dc.contributor.authorYu, B-
dc.contributor.authorLin, Y-
dc.contributor.authorRan, X-
dc.contributor.authorLai, WH-
dc.contributor.authorChu, M-
dc.contributor.authorHu, Y-
dc.contributor.authorYang, J-
dc.contributor.authorTse, HF-
dc.date.accessioned2021-11-12T13:36:10Z-
dc.date.available2021-11-12T13:36:10Z-
dc.date.issued2021-
dc.identifier.citationStem Cell Research, 2021, v. 50, article no. 102120-
dc.identifier.issn1873-5061-
dc.identifier.urihttp://hdl.handle.net/10722/307676-
dc.description.abstractThe human transcription factor NKX2-5 plays an important role in cardiac formation and development, and thus it can be used for isolation of cardiomyocytes (CMs) differentiated from human pluripotent stem cells (hPSCs). Here, we knocked-in enhanced GFP (eGFP) and Pac (a puromycin resistant gene; PuroR) into the exon 1 coding region of NKX2-5 from a human iPSC line iPSN0003 using TALENs. The generated GIBHi002-A-2 enables us to monitor and optimize cardiac differentiation procedures via the cardiac progenitor cells (CPCs), as well as to isolate iPSC-derived CMs for drug screening.-
dc.languageeng-
dc.publisherElsevier BV. The Journal's web site is located at https://journals.elsevier.com/stem-cell-research-
dc.relation.ispartofStem Cell Research-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleGeneration of a human iPSC line GIBHi002-A-2 with a dual-reporter for NKX2-5 using TALENs-
dc.typeArticle-
dc.identifier.emailTse, HF: hftse@hkucc.hku.hk-
dc.identifier.authorityTse, HF=rp00428-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1016/j.scr.2020.102120-
dc.identifier.pmid33352530-
dc.identifier.scopuseid_2-s2.0-85098470406-
dc.identifier.hkuros329271-
dc.identifier.volume50-
dc.identifier.spagearticle no. 102120-
dc.identifier.epagearticle no. 102120-
dc.identifier.isiWOS:000614260000012-
dc.publisher.placeNetherlands-

Export via OAI-PMH Interface in XML Formats

OR

Export to Other Non-XML Formats