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Article: MicroRNAs regulate the sesquiterpenoid hormonal pathway in Drosophila and other arthropods

TitleMicroRNAs regulate the sesquiterpenoid hormonal pathway in Drosophila and other arthropods
Authors
KeywordsArthropod
Sesquiterpenoids
Juvenile hormone acid methyltransferase
Methoprene-tolerant
Evolution
MicroRNA
Issue Date2017
Citation
Proceedings of the Royal Society B: Biological Sciences, 2017, v. 284, n. 1869, article no. 20171827 How to Cite?
AbstractArthropods comprise the majority of all described animal species, and understanding their evolution is a central question in biology. Their developmental processes are under the precise control of distinct hormonal regulators, including the sesquiterpenoids juvenile hormone (JH) and methyl farnesoate. The control of the synthesis and mode of action of these hormones played important roles in the evolution of arthropods and their adaptation to diverse habitats. However, the precise roles of noncoding RNAs, such as microRNAs (miRNAs), controlling arthropod hormonal pathways are unknown. Here, we investigated the miRNA regulation of the expression of the juvenile hormone acid methyltransferase gene (JHAMT), which encodes a rate-determining sesquiterpenoid biosynthetic enzyme. Loss of function of the miRNA bantam in the fly Drosophila melanogaster increased JHAMT expression, while overexpression of the bantam repressed JHAMT expression and resulted in pupal lethality. The male genital organs of the pupae were malformed, and exogenous sesquiterpenoid application partially rescued the genital deformities. The role of the bantam in the regulation of sesquiterpenoid biosynthesis was validated by transcriptomic, qPCR and hormone titre (JHB3 and JH III) analyses. In addition, we found a conserved set of miRNAs that interacted with JHAMT, and the sesquiterpenoid receptor methoprene-tolerant (Met) in different arthropod lineages, including insects (fly, mosquito and beetle), crustaceans (water flea and shrimp), myriapod (centipede) and chelicerate (horseshoe crab). This suggests that these miRNAs might have conserved roles in the post-transcriptional regulation of genes in sesquiterpenoid pathways across the Panarthropoda. Some of the identified lineage-specific miRNAs are potential targets for the development of new strategies in aquaculture and agricultural pest control.
Persistent Identifierhttp://hdl.handle.net/10722/307224
ISSN
2023 Impact Factor: 3.8
2023 SCImago Journal Rankings: 1.692
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorQu, Zhe-
dc.contributor.authorBendena, William G.-
dc.contributor.authorNong, Wenyan-
dc.contributor.authorSiggens, Kenneth W.-
dc.contributor.authorNoriega, Fernando G.-
dc.contributor.authorKai, Zhen Peng-
dc.contributor.authorZang, Yang Yang-
dc.contributor.authorKoon, Alex C.-
dc.contributor.authorChan, Ho Yin Edwin-
dc.contributor.authorChan, Ting Fung-
dc.contributor.authorChu, Ka Hou-
dc.contributor.authorLam, Hon Ming-
dc.contributor.authorAkam, Michael-
dc.contributor.authorTobe, Stephen S.-
dc.contributor.authorHui, Jerome Ho Lam-
dc.date.accessioned2021-11-03T06:22:11Z-
dc.date.available2021-11-03T06:22:11Z-
dc.date.issued2017-
dc.identifier.citationProceedings of the Royal Society B: Biological Sciences, 2017, v. 284, n. 1869, article no. 20171827-
dc.identifier.issn0962-8452-
dc.identifier.urihttp://hdl.handle.net/10722/307224-
dc.description.abstractArthropods comprise the majority of all described animal species, and understanding their evolution is a central question in biology. Their developmental processes are under the precise control of distinct hormonal regulators, including the sesquiterpenoids juvenile hormone (JH) and methyl farnesoate. The control of the synthesis and mode of action of these hormones played important roles in the evolution of arthropods and their adaptation to diverse habitats. However, the precise roles of noncoding RNAs, such as microRNAs (miRNAs), controlling arthropod hormonal pathways are unknown. Here, we investigated the miRNA regulation of the expression of the juvenile hormone acid methyltransferase gene (JHAMT), which encodes a rate-determining sesquiterpenoid biosynthetic enzyme. Loss of function of the miRNA bantam in the fly Drosophila melanogaster increased JHAMT expression, while overexpression of the bantam repressed JHAMT expression and resulted in pupal lethality. The male genital organs of the pupae were malformed, and exogenous sesquiterpenoid application partially rescued the genital deformities. The role of the bantam in the regulation of sesquiterpenoid biosynthesis was validated by transcriptomic, qPCR and hormone titre (JHB3 and JH III) analyses. In addition, we found a conserved set of miRNAs that interacted with JHAMT, and the sesquiterpenoid receptor methoprene-tolerant (Met) in different arthropod lineages, including insects (fly, mosquito and beetle), crustaceans (water flea and shrimp), myriapod (centipede) and chelicerate (horseshoe crab). This suggests that these miRNAs might have conserved roles in the post-transcriptional regulation of genes in sesquiterpenoid pathways across the Panarthropoda. Some of the identified lineage-specific miRNAs are potential targets for the development of new strategies in aquaculture and agricultural pest control.-
dc.languageeng-
dc.relation.ispartofProceedings of the Royal Society B: Biological Sciences-
dc.subjectArthropod-
dc.subjectSesquiterpenoids-
dc.subjectJuvenile hormone acid methyltransferase-
dc.subjectMethoprene-tolerant-
dc.subjectEvolution-
dc.subjectMicroRNA-
dc.titleMicroRNAs regulate the sesquiterpenoid hormonal pathway in Drosophila and other arthropods-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1098/rspb.2017.1827-
dc.identifier.pmid29237851-
dc.identifier.pmcidPMC5745405-
dc.identifier.scopuseid_2-s2.0-85038820781-
dc.identifier.volume284-
dc.identifier.issue1869-
dc.identifier.spagearticle no. 20171827-
dc.identifier.epagearticle no. 20171827-
dc.identifier.eissn1471-2954-
dc.identifier.isiWOS:000418463000008-

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