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- Publisher Website: 10.1007/s11103-011-9798-5
- Scopus: eid_2-s2.0-80051871023
- PMID: 21643845
- WOS: WOS:000294200700009
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Article: An improved particle bombardment for the generation of transgenic plants by direct immobilization of relleasable Tn5 transposases onto gold particles
Title | An improved particle bombardment for the generation of transgenic plants by direct immobilization of relleasable Tn5 transposases onto gold particles |
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Authors | |
Keywords | Intein-tag Transformation frequency Tn5 transposase 6×Cys-tag Single copy insertion |
Issue Date | 2011 |
Citation | Plant Molecular Biology, 2011, v. 77, n. 1, p. 117-127 How to Cite? |
Abstract | We have developed a modified particle bombardment method for plant transgenesis. An intein-tag and a 6×Cys-tag were successively fused to the N-terminus of a hyperactive Tn5 transposase. The modified transposase was immobilized on bare gold microscopic particles via covalent binding of a 6×Cys-tag sulfydryl groups to the gold surface. The tethered transposase can bind the transposon DNA in vitro to form the transposome in the absence of Mg 2+ ions. After bombardment of the gold particles carrying the transposomes into the plant cells, the transposomes will be released from the carrier due to the activated self-cleavage function of intein-tag. Our data showed this procedure integrated foreign DNA into the plant genome with an increased transformation frequency as compared to the conventional particle bombardment method. A single copy insertion can also be obtained by decreasing of the assembled transposon DNA amount in relation to plant cell biomass. © 2011 Springer Science+Business Media B.V. |
Persistent Identifier | http://hdl.handle.net/10722/307120 |
ISSN | 2023 Impact Factor: 3.9 2023 SCImago Journal Rankings: 1.151 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Wu, Jun | - |
dc.contributor.author | Du, Hongwei | - |
dc.contributor.author | Liao, Xuewei | - |
dc.contributor.author | Zhao, Yu | - |
dc.contributor.author | Li, Liguan | - |
dc.contributor.author | Yang, Liuyan | - |
dc.date.accessioned | 2021-11-03T06:21:58Z | - |
dc.date.available | 2021-11-03T06:21:58Z | - |
dc.date.issued | 2011 | - |
dc.identifier.citation | Plant Molecular Biology, 2011, v. 77, n. 1, p. 117-127 | - |
dc.identifier.issn | 0167-4412 | - |
dc.identifier.uri | http://hdl.handle.net/10722/307120 | - |
dc.description.abstract | We have developed a modified particle bombardment method for plant transgenesis. An intein-tag and a 6×Cys-tag were successively fused to the N-terminus of a hyperactive Tn5 transposase. The modified transposase was immobilized on bare gold microscopic particles via covalent binding of a 6×Cys-tag sulfydryl groups to the gold surface. The tethered transposase can bind the transposon DNA in vitro to form the transposome in the absence of Mg 2+ ions. After bombardment of the gold particles carrying the transposomes into the plant cells, the transposomes will be released from the carrier due to the activated self-cleavage function of intein-tag. Our data showed this procedure integrated foreign DNA into the plant genome with an increased transformation frequency as compared to the conventional particle bombardment method. A single copy insertion can also be obtained by decreasing of the assembled transposon DNA amount in relation to plant cell biomass. © 2011 Springer Science+Business Media B.V. | - |
dc.language | eng | - |
dc.relation.ispartof | Plant Molecular Biology | - |
dc.subject | Intein-tag | - |
dc.subject | Transformation frequency | - |
dc.subject | Tn5 transposase | - |
dc.subject | 6×Cys-tag | - |
dc.subject | Single copy insertion | - |
dc.title | An improved particle bombardment for the generation of transgenic plants by direct immobilization of relleasable Tn5 transposases onto gold particles | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1007/s11103-011-9798-5 | - |
dc.identifier.pmid | 21643845 | - |
dc.identifier.scopus | eid_2-s2.0-80051871023 | - |
dc.identifier.volume | 77 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | 117 | - |
dc.identifier.epage | 127 | - |
dc.identifier.isi | WOS:000294200700009 | - |