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Book Chapter: Synchronization of HeLa Cells

TitleSynchronization of HeLa Cells
Authors
Keywordssynchronization
cyclin
Cell cycle
cyclin-dependent kinases
flow cytometry
Issue Date2011
PublisherHumana Press.
Citation
Synchronization of HeLa Cells. In Banfalvi, G (Ed.), Cell Cycle Synchronization: Methods and Protocols, p. 151-161. New York, NY: Humana Press, 2011 How to Cite?
AbstractHeLa is one of the oldest and most commonly used cell lines in biomedical research. Owing to the ease of which they can be effectively synchronized by various methods, HeLa cells have been used extensively for studies of the cell cycle. Here we describe several protocols for synchronization of HeLa cells from different phases of the cell cycle. Synchronization in G 1 phase can be achieved with the HMG-CoA reductase inhibitor lovastatin, S phase with a double thymidine block procedure, and G 2 phase with the CDK inhibitor RO3306. Cells can also be enriched in mitosis by treating with nocodazole and mechanical shake-off. Release of the cells from these blocks enables researchers to follow gene expression and other events through the cell cycle. We also describe several protocols, including flow cytometry, BrdU labeling, immunoblotting, and time-lapse microscopy, for validating the synchrony of the cells and monitoring the progression of the cell cycle after release. © 2011 Springer Science+Business Media, LLC.
Persistent Identifierhttp://hdl.handle.net/10722/307119
ISBN
ISSN
2023 SCImago Journal Rankings: 0.399
Series/Report no.Methods in Molecular Biology ; 761

 

DC FieldValueLanguage
dc.contributor.authorMa, Hoi Tang-
dc.contributor.authorPoon, Randy Y.C.-
dc.date.accessioned2021-11-03T06:21:58Z-
dc.date.available2021-11-03T06:21:58Z-
dc.date.issued2011-
dc.identifier.citationSynchronization of HeLa Cells. In Banfalvi, G (Ed.), Cell Cycle Synchronization: Methods and Protocols, p. 151-161. New York, NY: Humana Press, 2011-
dc.identifier.isbn9781617791819-
dc.identifier.issn1064-3745-
dc.identifier.urihttp://hdl.handle.net/10722/307119-
dc.description.abstractHeLa is one of the oldest and most commonly used cell lines in biomedical research. Owing to the ease of which they can be effectively synchronized by various methods, HeLa cells have been used extensively for studies of the cell cycle. Here we describe several protocols for synchronization of HeLa cells from different phases of the cell cycle. Synchronization in G 1 phase can be achieved with the HMG-CoA reductase inhibitor lovastatin, S phase with a double thymidine block procedure, and G 2 phase with the CDK inhibitor RO3306. Cells can also be enriched in mitosis by treating with nocodazole and mechanical shake-off. Release of the cells from these blocks enables researchers to follow gene expression and other events through the cell cycle. We also describe several protocols, including flow cytometry, BrdU labeling, immunoblotting, and time-lapse microscopy, for validating the synchrony of the cells and monitoring the progression of the cell cycle after release. © 2011 Springer Science+Business Media, LLC.-
dc.languageeng-
dc.publisherHumana Press.-
dc.relation.ispartofCell Cycle Synchronization: Methods and Protocols-
dc.relation.ispartofseriesMethods in Molecular Biology ; 761-
dc.subjectsynchronization-
dc.subjectcyclin-
dc.subjectCell cycle-
dc.subjectcyclin-dependent kinases-
dc.subjectflow cytometry-
dc.titleSynchronization of HeLa Cells-
dc.typeBook_Chapter-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/978-1-61779-182-6_10-
dc.identifier.pmid21755447-
dc.identifier.scopuseid_2-s2.0-79960937456-
dc.identifier.spage151-
dc.identifier.epage161-
dc.publisher.placeNew York, NY-

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