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Article: Multi-target electrochemical malaria aptasensor on flexible multielectrode arrays for detection in malaria parasite blood samples

TitleMulti-target electrochemical malaria aptasensor on flexible multielectrode arrays for detection in malaria parasite blood samples
Authors
KeywordsFlexible polymer substrate
Multielectrode arrays
Multi-target detection
Malaria detection
Electrochemical aptasensor
Issue Date2021
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/snb
Citation
Sensors and Actuators B: Chemical, 2021, v. 349, p. article no. 130812 How to Cite?
AbstractDespite all efforts, malaria is still highly prevalent in tropical and developing countries. The “test, treat and track” policy of the World Health Organization (WHO) demands the development of affordable and highly sensitive malaria tests that discriminate between the two common malaria parasites, Plasmodium falciparum and Plasmodium vivax, to guide appropriate treatments. In response, we developed a flexible and disposable multielectrode array utilized as electrochemical malaria aptasensor. This multi-target aptasensor was modified by four different aptamer receptors, discriminating between P. falciparum and P. vivax infections via Plasmodium lactate dehydrogenase, expressed by both species, and histidine-rich protein 2, exclusively expressed by P. falciparum. The multi-target aptasensor detection was tested in blood samples spiked with target proteins, whole human blood spiked with P. falciparum, and P. falciparum in vitro cultures. A rigorous analysis revealed sensitivities of >75.0% for 0.001% parasitemia (50 parasites/µL) and a logic gate-based discrimination of Plasmodium infections, overcoming WHO standards. A cost analysis further substantiated the applicability of this multi-target aptasensor as a disposable point-of-care test for remote areas dealing with prevalent malaria parasite infections.
Persistent Identifierhttp://hdl.handle.net/10722/306318
ISSN
2023 Impact Factor: 8.0
2023 SCImago Journal Rankings: 1.475
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorFigueroa-Miranda, G-
dc.contributor.authorChen, S-
dc.contributor.authorNeis, M-
dc.contributor.authorZhou, L-
dc.contributor.authorZhang, Y-
dc.contributor.authorLO, Y-
dc.contributor.authorTanner, JA-
dc.contributor.authorKreidenweiss, A-
dc.contributor.authorOffenhäusser, A-
dc.contributor.authorMayer, D-
dc.date.accessioned2021-10-20T10:21:55Z-
dc.date.available2021-10-20T10:21:55Z-
dc.date.issued2021-
dc.identifier.citationSensors and Actuators B: Chemical, 2021, v. 349, p. article no. 130812-
dc.identifier.issn0925-4005-
dc.identifier.urihttp://hdl.handle.net/10722/306318-
dc.description.abstractDespite all efforts, malaria is still highly prevalent in tropical and developing countries. The “test, treat and track” policy of the World Health Organization (WHO) demands the development of affordable and highly sensitive malaria tests that discriminate between the two common malaria parasites, Plasmodium falciparum and Plasmodium vivax, to guide appropriate treatments. In response, we developed a flexible and disposable multielectrode array utilized as electrochemical malaria aptasensor. This multi-target aptasensor was modified by four different aptamer receptors, discriminating between P. falciparum and P. vivax infections via Plasmodium lactate dehydrogenase, expressed by both species, and histidine-rich protein 2, exclusively expressed by P. falciparum. The multi-target aptasensor detection was tested in blood samples spiked with target proteins, whole human blood spiked with P. falciparum, and P. falciparum in vitro cultures. A rigorous analysis revealed sensitivities of >75.0% for 0.001% parasitemia (50 parasites/µL) and a logic gate-based discrimination of Plasmodium infections, overcoming WHO standards. A cost analysis further substantiated the applicability of this multi-target aptasensor as a disposable point-of-care test for remote areas dealing with prevalent malaria parasite infections.-
dc.languageeng-
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/snb-
dc.relation.ispartofSensors and Actuators B: Chemical-
dc.subjectFlexible polymer substrate-
dc.subjectMultielectrode arrays-
dc.subjectMulti-target detection-
dc.subjectMalaria detection-
dc.subjectElectrochemical aptasensor-
dc.titleMulti-target electrochemical malaria aptasensor on flexible multielectrode arrays for detection in malaria parasite blood samples-
dc.typeArticle-
dc.identifier.emailTanner, JA: jatanner@hkucc.hku.hk-
dc.identifier.authorityTanner, JA=rp00495-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.snb.2021.130812-
dc.identifier.scopuseid_2-s2.0-85116379501-
dc.identifier.hkuros327633-
dc.identifier.volume349-
dc.identifier.spagearticle no. 130812-
dc.identifier.epagearticle no. 130812-
dc.identifier.isiWOS:000707315100008-
dc.publisher.placeNetherlands-

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