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Article: Controllable ligand spacing stimulates cellular mechanotransduction and promotes stem cell osteogenic differentiation on soft hydrogels

TitleControllable ligand spacing stimulates cellular mechanotransduction and promotes stem cell osteogenic differentiation on soft hydrogels
Authors
KeywordsLigand spacing
Mechanotransduction
Mesenchymal stem cell
Differentiation
Hydrogel
Issue Date2021
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biomaterials
Citation
Biomaterials, 2021, v. 268, p. article no. 120543 How to Cite?
AbstractHydrogels with tunable mechanical properties have provided a tremendous opportunity to regulate stem cell differentiation. Hydrogels with osteoid (about 30–40 kPa) or higher stiffness are usually required to induce the osteogenic differentiation of mesenchymal stem cells (MSCs). It is yet difficult to achieve the same differentiation on very soft hydrogels, because of low environmental mechanical stimuli and restricted cellular mechanotransduction. Here, we modulate cellular spatial sensing of integrin-adhesive ligands via quasi-hexagonally arranged nanopatterns to promote cell mechanosensing on hydrogels having low stiffness (about 3 kPa). The increased interligand spacing has been shown to regulate actomyosin force loading to recruit extra integrins on soft hydrogels. It therefore activates mechanotransduction and promotes the osteogenic differentiation of MSCs on soft hydrogels to the level comparable with the one observed on osteoid stiffness. Our work opens up new possibilities for the design of biomaterials and tissue scaffolds for regenerative therapeutics.
Persistent Identifierhttp://hdl.handle.net/10722/305816
ISSN
2023 Impact Factor: 12.8
2023 SCImago Journal Rankings: 3.016
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhang, M-
dc.contributor.authorSun, Q-
dc.contributor.authorLiu, Y-
dc.contributor.authorChu, Z-
dc.contributor.authorYu, L-
dc.contributor.authorHou, Y-
dc.contributor.authorKang, H-
dc.contributor.authorWei, Q-
dc.contributor.authorZhao, W-
dc.contributor.authorSpatz, JP-
dc.contributor.authorZhao, C-
dc.contributor.authorCavalcanti-Adam, EA-
dc.date.accessioned2021-10-20T10:14:43Z-
dc.date.available2021-10-20T10:14:43Z-
dc.date.issued2021-
dc.identifier.citationBiomaterials, 2021, v. 268, p. article no. 120543-
dc.identifier.issn0142-9612-
dc.identifier.urihttp://hdl.handle.net/10722/305816-
dc.description.abstractHydrogels with tunable mechanical properties have provided a tremendous opportunity to regulate stem cell differentiation. Hydrogels with osteoid (about 30–40 kPa) or higher stiffness are usually required to induce the osteogenic differentiation of mesenchymal stem cells (MSCs). It is yet difficult to achieve the same differentiation on very soft hydrogels, because of low environmental mechanical stimuli and restricted cellular mechanotransduction. Here, we modulate cellular spatial sensing of integrin-adhesive ligands via quasi-hexagonally arranged nanopatterns to promote cell mechanosensing on hydrogels having low stiffness (about 3 kPa). The increased interligand spacing has been shown to regulate actomyosin force loading to recruit extra integrins on soft hydrogels. It therefore activates mechanotransduction and promotes the osteogenic differentiation of MSCs on soft hydrogels to the level comparable with the one observed on osteoid stiffness. Our work opens up new possibilities for the design of biomaterials and tissue scaffolds for regenerative therapeutics.-
dc.languageeng-
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biomaterials-
dc.relation.ispartofBiomaterials-
dc.subjectLigand spacing-
dc.subjectMechanotransduction-
dc.subjectMesenchymal stem cell-
dc.subjectDifferentiation-
dc.subjectHydrogel-
dc.titleControllable ligand spacing stimulates cellular mechanotransduction and promotes stem cell osteogenic differentiation on soft hydrogels-
dc.typeArticle-
dc.identifier.emailChu, Z: zqchu@eee.hku.hk-
dc.identifier.authorityChu, Z=rp02472-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.biomaterials.2020.120543-
dc.identifier.pmid33260094-
dc.identifier.scopuseid_2-s2.0-85096875227-
dc.identifier.hkuros328148-
dc.identifier.volume268-
dc.identifier.spagearticle no. 120543-
dc.identifier.epagearticle no. 120543-
dc.identifier.isiWOS:000611884700003-
dc.publisher.placeNetherlands-

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