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Article: Biofabrication of endothelial cell, dermal fibroblast, and multilayered keratinocyte layers for skin tissue engineering

TitleBiofabrication of endothelial cell, dermal fibroblast, and multilayered keratinocyte layers for skin tissue engineering
Authors
Keywordsbioprinting
dermal fibroblasts
gelatin methacryloyl (GelMA)
multilayered keratinocytes
skin tissue engineering
Issue Date2021
PublisherInstitute of Physics Publishing Ltd. The Journal's web site is located at http://www.iop.org/EJ/journal/bf
Citation
Biofabrication, 2021, v. 13 n. 3, p. article no. 035030 How to Cite?
AbstractThe skin serves a substantial number of physiological purposes and is exposed to numerous biological and chemical agents owing to its large surface area and accessibility. Yet, current skin models are limited in emulating the multifaceted functions of skin tissues due to a lack of effort on the optimization of biomaterials and techniques at different skin layers for building skin frameworks. Here, we use biomaterial-based approaches and bioengineered techniques to develop a 3D skin model with layers of endothelial cell networks, dermal fibroblasts, and multilayered keratinocytes. Analysis of mechanical properties of gelatin methacryloyl (GelMA)-based bioinks mixed with different portions of alginate revealed bioprinted endothelium could be better modeled to optimize endothelial cell viability with a mixture of 7.5% GelMA and 2% alginate. Matrix stiffness plays a crucial role in modulating produced levels of Pro-Collagen I alpha-1 and matrix metalloproteinase-1 in human dermal fibroblasts and affecting their viability, proliferation, and spreading. Moreover, seeding human keratinocytes with gelatin-coating multiple times proved to be helpful in reducing culture time to create multiple layers of keratinocytes while maintaining their viability. The ability to fabricate selected biomaterials for each layer of skin tissues has implications in the biofabrication of skin systems for regenerative medicine and disease modeling.
Persistent Identifierhttp://hdl.handle.net/10722/305815
ISSN
2023 Impact Factor: 8.2
2023 SCImago Journal Rankings: 1.769
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBarros, N-
dc.contributor.authorKim, HJ-
dc.contributor.authorGoudie, MJ-
dc.contributor.authorLee, K-
dc.contributor.authorBandaru, P-
dc.contributor.authorBanton, EA-
dc.contributor.authorSarikhani, E-
dc.contributor.authorSun, W-
dc.contributor.authorZhang, S-
dc.contributor.authorCho, HJ-
dc.contributor.authorHartel, MC-
dc.contributor.authorOstrovidov, S-
dc.contributor.authorAhadian, S-
dc.contributor.authorHussain, SM-
dc.contributor.authorAshammakhi, N-
dc.contributor.authorDokmeci, M-
dc.contributor.authorHerculano, RD-
dc.contributor.authorLee, J-
dc.contributor.authorKhademhosseini, A-
dc.date.accessioned2021-10-20T10:14:42Z-
dc.date.available2021-10-20T10:14:42Z-
dc.date.issued2021-
dc.identifier.citationBiofabrication, 2021, v. 13 n. 3, p. article no. 035030-
dc.identifier.issn1758-5082-
dc.identifier.urihttp://hdl.handle.net/10722/305815-
dc.description.abstractThe skin serves a substantial number of physiological purposes and is exposed to numerous biological and chemical agents owing to its large surface area and accessibility. Yet, current skin models are limited in emulating the multifaceted functions of skin tissues due to a lack of effort on the optimization of biomaterials and techniques at different skin layers for building skin frameworks. Here, we use biomaterial-based approaches and bioengineered techniques to develop a 3D skin model with layers of endothelial cell networks, dermal fibroblasts, and multilayered keratinocytes. Analysis of mechanical properties of gelatin methacryloyl (GelMA)-based bioinks mixed with different portions of alginate revealed bioprinted endothelium could be better modeled to optimize endothelial cell viability with a mixture of 7.5% GelMA and 2% alginate. Matrix stiffness plays a crucial role in modulating produced levels of Pro-Collagen I alpha-1 and matrix metalloproteinase-1 in human dermal fibroblasts and affecting their viability, proliferation, and spreading. Moreover, seeding human keratinocytes with gelatin-coating multiple times proved to be helpful in reducing culture time to create multiple layers of keratinocytes while maintaining their viability. The ability to fabricate selected biomaterials for each layer of skin tissues has implications in the biofabrication of skin systems for regenerative medicine and disease modeling.-
dc.languageeng-
dc.publisherInstitute of Physics Publishing Ltd. The Journal's web site is located at http://www.iop.org/EJ/journal/bf-
dc.relation.ispartofBiofabrication-
dc.rightsBiofabrication. Copyright © Institute of Physics Publishing Ltd.-
dc.rightsThis is an author-created, un-copyedited version of an article published in [insert name of journal]. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at http://dx.doi.org/[insert DOI].-
dc.subjectbioprinting-
dc.subjectdermal fibroblasts-
dc.subjectgelatin methacryloyl (GelMA)-
dc.subjectmultilayered keratinocytes-
dc.subjectskin tissue engineering-
dc.titleBiofabrication of endothelial cell, dermal fibroblast, and multilayered keratinocyte layers for skin tissue engineering-
dc.typeArticle-
dc.identifier.emailZhang, S: beszhang@hku.hk-
dc.identifier.authorityZhang, S=rp02764-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1088/1758-5090/aba503-
dc.identifier.pmid32650324-
dc.identifier.scopuseid_2-s2.0-85100605965-
dc.identifier.hkuros328141-
dc.identifier.volume13-
dc.identifier.issue3-
dc.identifier.spagearticle no. 035030-
dc.identifier.epagearticle no. 035030-
dc.identifier.isiWOS:000639932800001-
dc.publisher.placeUnited Kingdom-

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