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Article: An electrochemical aptamer-based biosensor targeting Plasmodium falciparum histidine-rich protein II for malaria diagnosis

TitleAn electrochemical aptamer-based biosensor targeting Plasmodium falciparum histidine-rich protein II for malaria diagnosis
Authors
KeywordsAptasensor
Electrochemical aptamer-based biosensor
Malaria
Plasmodium falciparum histidine-Rich protein II
Rapid diagnostic test
Issue Date2021
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bios
Citation
Biosensors and Bioelectronics, 2021, v. 192, p. article no. 113472 How to Cite?
AbstractMalaria is an infectious disease caused by parasitic protozoans from the genus Plasmodium, with the species P. falciparum causing the highest number of deaths worldwide. Rapid diagnostic tests (RDTs) have become critical in the management of malaria, but current RDTs that detect P. falciparum are primarily antibody-based, which can have drawbacks in cost and robustness. Here, we report the development of an electrochemical aptamer-based (E-AB) biosensing alternative. Through selective evolution of ligands by exponential enrichment, we identify DNA aptamers that bind specifically to P. falciparum histidine-rich protein II (PfHRP2). The aptamer is modified with a methylene blue reporter and attached to a gold sensor surface for square-wave voltammetry interrogation. Through this method we are able to quantify PfHRP2 in human serum with an LOD of 3.73 nM. We further demonstrate the biosensor is stable in serum buffers and reusable for multiple detection rounds. These findings provide a promising alternative to conventional PfHRP2 detection for malaria diagnosis, while also expanding the capabilities of E-AB biosensors.
Persistent Identifierhttp://hdl.handle.net/10722/305739
ISSN
2020 Impact Factor: 10.618
2020 SCImago Journal Rankings: 2.546
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLO, Y-
dc.contributor.authorCheung, YW-
dc.contributor.authorWANG, L-
dc.contributor.authorLee, M-
dc.contributor.authorFigueroa-Miranda, G-
dc.contributor.authorLiang, S-
dc.contributor.authorMayer, D-
dc.contributor.authorTanner, JA-
dc.date.accessioned2021-10-20T10:13:38Z-
dc.date.available2021-10-20T10:13:38Z-
dc.date.issued2021-
dc.identifier.citationBiosensors and Bioelectronics, 2021, v. 192, p. article no. 113472-
dc.identifier.issn0956-5663-
dc.identifier.urihttp://hdl.handle.net/10722/305739-
dc.description.abstractMalaria is an infectious disease caused by parasitic protozoans from the genus Plasmodium, with the species P. falciparum causing the highest number of deaths worldwide. Rapid diagnostic tests (RDTs) have become critical in the management of malaria, but current RDTs that detect P. falciparum are primarily antibody-based, which can have drawbacks in cost and robustness. Here, we report the development of an electrochemical aptamer-based (E-AB) biosensing alternative. Through selective evolution of ligands by exponential enrichment, we identify DNA aptamers that bind specifically to P. falciparum histidine-rich protein II (PfHRP2). The aptamer is modified with a methylene blue reporter and attached to a gold sensor surface for square-wave voltammetry interrogation. Through this method we are able to quantify PfHRP2 in human serum with an LOD of 3.73 nM. We further demonstrate the biosensor is stable in serum buffers and reusable for multiple detection rounds. These findings provide a promising alternative to conventional PfHRP2 detection for malaria diagnosis, while also expanding the capabilities of E-AB biosensors.-
dc.languageeng-
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bios-
dc.relation.ispartofBiosensors and Bioelectronics-
dc.subjectAptasensor-
dc.subjectElectrochemical aptamer-based biosensor-
dc.subjectMalaria-
dc.subjectPlasmodium falciparum histidine-Rich protein II-
dc.subjectRapid diagnostic test-
dc.titleAn electrochemical aptamer-based biosensor targeting Plasmodium falciparum histidine-rich protein II for malaria diagnosis-
dc.typeArticle-
dc.identifier.emailTanner, JA: jatanner@hkucc.hku.hk-
dc.identifier.authorityTanner, JA=rp00495-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.bios.2021.113472-
dc.identifier.pmid34271397-
dc.identifier.scopuseid_2-s2.0-85109915771-
dc.identifier.hkuros327622-
dc.identifier.volume192-
dc.identifier.spagearticle no. 113472-
dc.identifier.epagearticle no. 113472-
dc.identifier.isiWOS:000704056300009-
dc.publisher.placeNetherlands-

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