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Article: The male germline-specific protein MAPS is indispensable for pachynema progression and fertility
Title | The male germline-specific protein MAPS is indispensable for pachynema progression and fertility |
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Authors | |
Keywords | male germline-specific protein meiosis prophase I pachynema progression MAPS |
Issue Date | 2021 |
Publisher | National Academy of Sciences. The Journal's web site is located at http://www.pnas.org |
Citation | Proceedings of the National Academy of Sciences, 2021, v. 118 n. 8, p. article no. e2025421118 How to Cite? |
Abstract | Meiosis is a specialized cell division that creates haploid germ cells from diploid progenitors. Through differential RNA expression analyses, we previously identified a number of mouse genes that were dramatically elevated in spermatocytes, relative to their very low expression in spermatogonia and somatic organs. Here, we investigated in detail 1700102P08Rik, one of these genes, and independently conclude that it encodes a male germline-specific protein, in agreement with a recent report. We demonstrated that it is essential for pachynema progression in spermatocytes and named it male pachynema-specific (MAPS) protein. Mice lacking Maps (Maps−/−) suffered from pachytene arrest and spermatocyte death, leading to male infertility, whereas female fertility was not affected. Interestingly, pubertal Maps−/− spermatocytes were arrested at early pachytene stage, accompanied by defects in DNA double-strand break (DSB) repair, crossover formation, and XY body formation. In contrast, adult Maps−/− spermatocytes only exhibited partially defective crossover but nonetheless were delayed or failed in progression from early to mid- and late pachytene stage, resulting in cell death. Furthermore, we report a significant transcriptional dysregulation in autosomes and XY chromosomes in both pubertal and adult Maps−/− pachytene spermatocytes, including failed meiotic sex chromosome inactivation (MSCI). Further experiments revealed that MAPS overexpression in vitro dramatically decreased the ubiquitination levels of cellular proteins. Conversely, in Maps−/− pachytene cells, protein ubiquitination was dramatically increased, likely contributing to the large-scale disruption in gene expression in pachytene cells. Thus, MAPS is a protein essential for pachynema progression in male mice, possibly in mammals in general. |
Description | Hybrid open access |
Persistent Identifier | http://hdl.handle.net/10722/305488 |
ISSN | 2023 Impact Factor: 9.4 2023 SCImago Journal Rankings: 3.737 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Li, M | - |
dc.contributor.author | ZHENG, J | - |
dc.contributor.author | Li, G | - |
dc.contributor.author | Lin, Z | - |
dc.contributor.author | LI, D | - |
dc.contributor.author | Liu, D | - |
dc.contributor.author | Feng, H | - |
dc.contributor.author | Cao, D | - |
dc.contributor.author | Ng, EHY | - |
dc.contributor.author | Li, RHW | - |
dc.contributor.author | Han, C | - |
dc.contributor.author | Yeung, WSB | - |
dc.contributor.author | Chow, LT | - |
dc.contributor.author | Wang, H | - |
dc.contributor.author | Liu, K | - |
dc.date.accessioned | 2021-10-20T10:10:06Z | - |
dc.date.available | 2021-10-20T10:10:06Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Proceedings of the National Academy of Sciences, 2021, v. 118 n. 8, p. article no. e2025421118 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | http://hdl.handle.net/10722/305488 | - |
dc.description | Hybrid open access | - |
dc.description.abstract | Meiosis is a specialized cell division that creates haploid germ cells from diploid progenitors. Through differential RNA expression analyses, we previously identified a number of mouse genes that were dramatically elevated in spermatocytes, relative to their very low expression in spermatogonia and somatic organs. Here, we investigated in detail 1700102P08Rik, one of these genes, and independently conclude that it encodes a male germline-specific protein, in agreement with a recent report. We demonstrated that it is essential for pachynema progression in spermatocytes and named it male pachynema-specific (MAPS) protein. Mice lacking Maps (Maps−/−) suffered from pachytene arrest and spermatocyte death, leading to male infertility, whereas female fertility was not affected. Interestingly, pubertal Maps−/− spermatocytes were arrested at early pachytene stage, accompanied by defects in DNA double-strand break (DSB) repair, crossover formation, and XY body formation. In contrast, adult Maps−/− spermatocytes only exhibited partially defective crossover but nonetheless were delayed or failed in progression from early to mid- and late pachytene stage, resulting in cell death. Furthermore, we report a significant transcriptional dysregulation in autosomes and XY chromosomes in both pubertal and adult Maps−/− pachytene spermatocytes, including failed meiotic sex chromosome inactivation (MSCI). Further experiments revealed that MAPS overexpression in vitro dramatically decreased the ubiquitination levels of cellular proteins. Conversely, in Maps−/− pachytene cells, protein ubiquitination was dramatically increased, likely contributing to the large-scale disruption in gene expression in pachytene cells. Thus, MAPS is a protein essential for pachynema progression in male mice, possibly in mammals in general. | - |
dc.language | eng | - |
dc.publisher | National Academy of Sciences. The Journal's web site is located at http://www.pnas.org | - |
dc.relation.ispartof | Proceedings of the National Academy of Sciences | - |
dc.rights | Proceedings of the National Academy of Sciences. Copyright © National Academy of Sciences. | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | male germline-specific protein | - |
dc.subject | meiosis prophase I | - |
dc.subject | pachynema progression | - |
dc.subject | MAPS | - |
dc.title | The male germline-specific protein MAPS is indispensable for pachynema progression and fertility | - |
dc.type | Article | - |
dc.identifier.email | Li, M: miaoli@hku.hk | - |
dc.identifier.email | Liu, D: liudt@hku.hk | - |
dc.identifier.email | Feng, H: fhw1010@hku.hk | - |
dc.identifier.email | Ng, EHY: nghye@hku.hk | - |
dc.identifier.email | Li, RHW: raymondli@hku.hk | - |
dc.identifier.email | Yeung, WSB: wsbyeung@hku.hk | - |
dc.identifier.email | Liu, K: kliugc@hku.hk | - |
dc.identifier.authority | Ng, EHY=rp00426 | - |
dc.identifier.authority | Li, RHW=rp01649 | - |
dc.identifier.authority | Yeung, WSB=rp00331 | - |
dc.identifier.authority | Liu, K=rp02475 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1073/pnas.2025421118 | - |
dc.identifier.pmid | 33602822 | - |
dc.identifier.pmcid | PMC7923350 | - |
dc.identifier.scopus | eid_2-s2.0-85101219166 | - |
dc.identifier.hkuros | 326643 | - |
dc.identifier.volume | 118 | - |
dc.identifier.issue | 8 | - |
dc.identifier.spage | article no. e2025421118 | - |
dc.identifier.epage | article no. e2025421118 | - |
dc.identifier.isi | WOS:000621797000084 | - |
dc.publisher.place | United States | - |