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Article: Generation of genomic-integration-free human induced pluripotent stem cells and the derived cardiomyocytes of X-linked dilated cardiomyopathy from DMD gene mutation

TitleGeneration of genomic-integration-free human induced pluripotent stem cells and the derived cardiomyocytes of X-linked dilated cardiomyopathy from DMD gene mutation
Authors
Issue Date2020
PublisherElsevier BV. The Journal's web site is located at https://journals.elsevier.com/stem-cell-research
Citation
Stem Cell Research, 2020, v. 49, article no. 102040 How to Cite?
AbstractWe derived an integration-free induced pluripotent stem cell (iPSC) line from the peripheral blood mononuclear cells (PBMCs) of a 23-year-old male patient. This patient carries a 5′ splice site point mutation in intron 1 (c.31+1G>A) of the dystrophin gene, a mutation associated with X-linked dilated cardiomyopathy (XLDCM). Sendai virus was used to reprogram the PBMCs and deliver OCT3/4, SOX2, c-MYC, and KLF4 factors. The iPSC line (HKUi002-A) generated preserved the mutation, expressed common pluripotency markers, differentiated into three germ layers in vivo, and exhibited a normal karyotype. Further differentiation into cardiomyocytes enables the study of the disease mechanisms of XLDCM.
Persistent Identifierhttp://hdl.handle.net/10722/305423
ISSN
2023 Impact Factor: 0.8
2023 SCImago Journal Rankings: 0.467
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhu, S-
dc.contributor.authorLaw, AHY-
dc.contributor.authorDeng, R-
dc.contributor.authorPoon, ENY-
dc.contributor.authorLo, CW-
dc.contributor.authorKwong, AKY-
dc.contributor.authorLiang, R-
dc.contributor.authorChan, KYK-
dc.contributor.authorWong, WL-
dc.contributor.authorTan-Un, KC-
dc.contributor.authorPijnappel, WWMP-
dc.contributor.authorChan, GCF-
dc.contributor.authorChan, SHS-
dc.date.accessioned2021-10-20T10:09:11Z-
dc.date.available2021-10-20T10:09:11Z-
dc.date.issued2020-
dc.identifier.citationStem Cell Research, 2020, v. 49, article no. 102040-
dc.identifier.issn1873-5061-
dc.identifier.urihttp://hdl.handle.net/10722/305423-
dc.description.abstractWe derived an integration-free induced pluripotent stem cell (iPSC) line from the peripheral blood mononuclear cells (PBMCs) of a 23-year-old male patient. This patient carries a 5′ splice site point mutation in intron 1 (c.31+1G>A) of the dystrophin gene, a mutation associated with X-linked dilated cardiomyopathy (XLDCM). Sendai virus was used to reprogram the PBMCs and deliver OCT3/4, SOX2, c-MYC, and KLF4 factors. The iPSC line (HKUi002-A) generated preserved the mutation, expressed common pluripotency markers, differentiated into three germ layers in vivo, and exhibited a normal karyotype. Further differentiation into cardiomyocytes enables the study of the disease mechanisms of XLDCM.-
dc.languageeng-
dc.publisherElsevier BV. The Journal's web site is located at https://journals.elsevier.com/stem-cell-research-
dc.relation.ispartofStem Cell Research-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleGeneration of genomic-integration-free human induced pluripotent stem cells and the derived cardiomyocytes of X-linked dilated cardiomyopathy from DMD gene mutation-
dc.typeArticle-
dc.identifier.emailLaw, AHY: lawanna@hkucc.hku.hk-
dc.identifier.emailKwong, AKY: kkyanna@hku.hk-
dc.identifier.emailChan, KYK: ykchanc@hku.hk-
dc.identifier.emailTan-Un, KC: kctanun@hkucc.hku.hk-
dc.identifier.emailChan, GCF: gcfchan@hku.hk-
dc.identifier.emailChan, SHS: sophehs@hku.hk-
dc.identifier.authorityPoon, ENY=rp02233-
dc.identifier.authorityChan, KYK=rp00453-
dc.identifier.authorityTan-Un, KC=rp00787-
dc.identifier.authorityChan, GCF=rp00431-
dc.identifier.authorityChan, SHS=rp02210-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1016/j.scr.2020.102040-
dc.identifier.pmid33099108-
dc.identifier.scopuseid_2-s2.0-85093103477-
dc.identifier.hkuros328081-
dc.identifier.volume49-
dc.identifier.spagearticle no. 102040-
dc.identifier.epagearticle no. 102040-
dc.identifier.isiWOS:000600952800041-
dc.publisher.placeNetherlands-

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