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Article: Dynamics of B cell repertoires and emergence of cross-reactive responses in patients with different severities of COVID-19

TitleDynamics of B cell repertoires and emergence of cross-reactive responses in patients with different severities of COVID-19
Authors
KeywordsSARS-CoV-2
COVID-19
B cell repertoires
BCR selection
BCR sharing
B cell clonal expansion
Antibody
Cross-reactivity
Issue Date2021
PublisherElsevier (Cell Press): OAJ. The Journal's web site is located at http://cell.com/cell-reports
Citation
Cell Reports, 2021, v. 35 n. 8, article no. 109173 How to Cite?
AbstractIndividuals with the 2019 coronavirus disease (COVID-19) show varying severity of the disease, ranging from asymptomatic to requiring intensive care. Although monoclonal antibodies specific to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been identified, we still lack an understanding of the overall landscape of B cell receptor (BCR) repertoires in individuals with COVID-19. We use high-throughput sequencing of bulk and plasma B cells collected at multiple time points during infection to characterize signatures of the B cell response to SARS-CoV-2 in 19 individuals. Using principled statistical approaches, we associate differential features of BCRs with different disease severity. We identify 38 significantly expanded clonal lineages shared among individuals as candidates for responses specific to SARS-CoV-2. Using single-cell sequencing, we verify the reactivity of BCRs shared among individuals to SARS-CoV-2 epitopes. Moreover, we identify the natural emergence of a BCR with cross-reactivity to SARS-CoV-1 and SARS-CoV-2 in some individuals. Our results provide insights important for development of rational therapies and vaccines against COVID-19.
Persistent Identifierhttp://hdl.handle.net/10722/305245
ISSN
2023 Impact Factor: 7.5
2023 SCImago Journal Rankings: 4.279
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMontague, Z-
dc.contributor.authorLv, H-
dc.contributor.authorOtwinowski, J-
dc.contributor.authorDeWitt, WS-
dc.contributor.authorIsacchini, G-
dc.contributor.authorYip, GK-
dc.contributor.authorNg, WW-
dc.contributor.authorTsang, OTY-
dc.contributor.authorYuan, M-
dc.contributor.authorLiu, H-
dc.contributor.authorWilson, IA-
dc.contributor.authorPeiris, JSM-
dc.contributor.authorWu, NC-
dc.contributor.authorNourmohammad, A-
dc.contributor.authorMok, CKP-
dc.date.accessioned2021-10-20T10:06:42Z-
dc.date.available2021-10-20T10:06:42Z-
dc.date.issued2021-
dc.identifier.citationCell Reports, 2021, v. 35 n. 8, article no. 109173-
dc.identifier.issn2211-1247-
dc.identifier.urihttp://hdl.handle.net/10722/305245-
dc.description.abstractIndividuals with the 2019 coronavirus disease (COVID-19) show varying severity of the disease, ranging from asymptomatic to requiring intensive care. Although monoclonal antibodies specific to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been identified, we still lack an understanding of the overall landscape of B cell receptor (BCR) repertoires in individuals with COVID-19. We use high-throughput sequencing of bulk and plasma B cells collected at multiple time points during infection to characterize signatures of the B cell response to SARS-CoV-2 in 19 individuals. Using principled statistical approaches, we associate differential features of BCRs with different disease severity. We identify 38 significantly expanded clonal lineages shared among individuals as candidates for responses specific to SARS-CoV-2. Using single-cell sequencing, we verify the reactivity of BCRs shared among individuals to SARS-CoV-2 epitopes. Moreover, we identify the natural emergence of a BCR with cross-reactivity to SARS-CoV-1 and SARS-CoV-2 in some individuals. Our results provide insights important for development of rational therapies and vaccines against COVID-19.-
dc.languageeng-
dc.publisherElsevier (Cell Press): OAJ. The Journal's web site is located at http://cell.com/cell-reports-
dc.relation.ispartofCell Reports-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectSARS-CoV-2-
dc.subjectCOVID-19-
dc.subjectB cell repertoires-
dc.subjectBCR selection-
dc.subjectBCR sharing-
dc.subjectB cell clonal expansion-
dc.subjectAntibody-
dc.subjectCross-reactivity-
dc.titleDynamics of B cell repertoires and emergence of cross-reactive responses in patients with different severities of COVID-19-
dc.typeArticle-
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hk-
dc.identifier.authorityPeiris, JSM=rp00410-
dc.identifier.authorityMok, CKP=rp01805-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1016/j.celrep.2021.109173-
dc.identifier.pmid33991510-
dc.identifier.pmcidPMC8106887-
dc.identifier.scopuseid_2-s2.0-85106305039-
dc.identifier.hkuros327444-
dc.identifier.volume35-
dc.identifier.issue8-
dc.identifier.spagearticle no. 109173-
dc.identifier.epagearticle no. 109173-
dc.identifier.isiWOS:000655611900019-
dc.publisher.placeUnited States-

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