File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Genetic variation in ABCB5 associates with risk of hepatocellular carcinoma

TitleGenetic variation in ABCB5 associates with risk of hepatocellular carcinoma
Authors
Keywordsdrug transporter
liver cancer
multidrug resistance
Issue Date2020
PublisherWiley Open Access for Foundation for Cellular and Molecular Medicine. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=1582-1838
Citation
Journal of Cellular and Molecular Medicine, 2020, v. 24 n. 18, p. 10705-10713 How to Cite?
AbstractExpression of ATP-binding cassette B5 (ABCB5) has been demonstrated to confer chemoresistance, enhance cancer stem cell properties and associate with poor prognosis in hepatocellular carcinoma (HCC). The aim of this study was to evaluate the genetic variations of ABCB5 in HCC patients with reference to healthy individuals and the clinicopathological significance. A pilot study has examined 20 out of 300 pairs HCC and paralleled blood samples using conventional sequencing method to cover all exons and exon/intron regions to investigate whether there will be novel variant sequence and mutation event. A total of 300 HCC and 300 healthy blood DNA samples were then examined by Sequenom MassARRAY genotyping and pyrosequencing for 38 SNP and 1 INDEL in ABCB5. Five novel SNPs were identified in ABCB5. Comparison of DNA from blood samples of HCC and healthy demonstrated that ABCB5 SNPs rs75494098, rs4721940 and rs10254317 were associated with HCC risk. Specific ABCB5 variants were associated with aggressive HCC features. SNP rs17143212 was significantly associated with ABCB5 expression level. Nonetheless, the paralleled blood and tumour DNA sequences from HCC patients indicated that ABCB5 mutation in tumours was not common and corroborated the TCGA data sets. In conclusion, ABCB5 genetic variants had significant association with HCC risk and aggressive tumour properties.
Persistent Identifierhttp://hdl.handle.net/10722/305047
ISSN
2023 Impact Factor: 4.3
2023 SCImago Journal Rankings: 1.207
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLeung, ICY-
dc.contributor.authorChong, CCN-
dc.contributor.authorCheung, TT-
dc.contributor.authorYeung, PC-
dc.contributor.authorNg, KKC-
dc.contributor.authorLai, PBS-
dc.contributor.authorChan, SL-
dc.contributor.authorChan, AWH-
dc.contributor.authorTang, PMK-
dc.contributor.authorCheung, ST-
dc.date.accessioned2021-10-05T02:38:59Z-
dc.date.available2021-10-05T02:38:59Z-
dc.date.issued2020-
dc.identifier.citationJournal of Cellular and Molecular Medicine, 2020, v. 24 n. 18, p. 10705-10713-
dc.identifier.issn1582-1838-
dc.identifier.urihttp://hdl.handle.net/10722/305047-
dc.description.abstractExpression of ATP-binding cassette B5 (ABCB5) has been demonstrated to confer chemoresistance, enhance cancer stem cell properties and associate with poor prognosis in hepatocellular carcinoma (HCC). The aim of this study was to evaluate the genetic variations of ABCB5 in HCC patients with reference to healthy individuals and the clinicopathological significance. A pilot study has examined 20 out of 300 pairs HCC and paralleled blood samples using conventional sequencing method to cover all exons and exon/intron regions to investigate whether there will be novel variant sequence and mutation event. A total of 300 HCC and 300 healthy blood DNA samples were then examined by Sequenom MassARRAY genotyping and pyrosequencing for 38 SNP and 1 INDEL in ABCB5. Five novel SNPs were identified in ABCB5. Comparison of DNA from blood samples of HCC and healthy demonstrated that ABCB5 SNPs rs75494098, rs4721940 and rs10254317 were associated with HCC risk. Specific ABCB5 variants were associated with aggressive HCC features. SNP rs17143212 was significantly associated with ABCB5 expression level. Nonetheless, the paralleled blood and tumour DNA sequences from HCC patients indicated that ABCB5 mutation in tumours was not common and corroborated the TCGA data sets. In conclusion, ABCB5 genetic variants had significant association with HCC risk and aggressive tumour properties.-
dc.languageeng-
dc.publisherWiley Open Access for Foundation for Cellular and Molecular Medicine. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=1582-1838-
dc.relation.ispartofJournal of Cellular and Molecular Medicine-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectdrug transporter-
dc.subjectliver cancer-
dc.subjectmultidrug resistance-
dc.titleGenetic variation in ABCB5 associates with risk of hepatocellular carcinoma-
dc.typeArticle-
dc.identifier.emailCheung, TT: cheung68@hku.hk-
dc.identifier.authorityCheung, TT=rp02129-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1111/jcmm.15691-
dc.identifier.pmid32783366-
dc.identifier.pmcidPMC7521249-
dc.identifier.scopuseid_2-s2.0-85089247408-
dc.identifier.hkuros326123-
dc.identifier.volume24-
dc.identifier.issue18-
dc.identifier.spage10705-
dc.identifier.epage10713-
dc.identifier.isiWOS:000558074900001-
dc.publisher.placeUnited Kingdom-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats