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Article: Optimised tissue clearing minimises distortion and destruction during tissue delipidation

TitleOptimised tissue clearing minimises distortion and destruction during tissue delipidation
Authors
Keywordsantigenicity
delipidation
opacity
protein loss
three-dimensional imaging
Issue Date2021
PublisherWiley-Blackwell Publishing Ltd. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-2990
Citation
Neuropathology and Applied Neurobiology, 2021, v. 47, p. 441-453 How to Cite?
AbstractAims: A variety of tissue clearing techniques have been developed to render intact tissue transparent. For thicker samples, additional partial tissue delipidation is re-quired before immersion into the final refractive index (RI)-matching solution, which alone is often inadequate to achieve full tissue transparency. However, it is difficult to determine a sufficient degree of tissue delipidation, excess of which can result in tissue distortion and protein loss. Here, we aim to develop a clearing strategy that allows better monitoring and more precise determination of delipidation progress.Methods: We combined the detergent sodium dodecyl sulphate (SDS) with OPTIClear, a RI-matching solution, to form a strategy termed Accurate delipidation with Optimal Clearing (Accu-OptiClearing). Accu-OptiClearing allows for a better preview of the final tissue transparency achieved when immersed in OPTIClear alone just before im-aging. We assessed for the changes in clearing rate, protein loss, degree of tissue dis-tortion, and preservation of antigens.Results: Partial delipidation using Accu-OptiClearing accelerated tissue clearing and better preserved tissue structure and antigens than delipidation with SDS alone. Despite achieving similar transparency in the final OPTIClear solution, more lipids were retained in samples cleared with Accu-OptiClearing compared to SDS.Conclusions: Combining the RI-matching solution OPTIClear with detergents, Accu-OptiClearing, can avoid excessive delipidation, leading to accelerated tissue clearing, less tissue damage and better preserved antigens.
Persistent Identifierhttp://hdl.handle.net/10722/304902
ISSN
2023 Impact Factor: 4.0
2023 SCImago Journal Rankings: 1.591
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLee, K-
dc.contributor.authorLai, HM-
dc.contributor.authorSoerensen, MH-
dc.contributor.authorHui, ESK-
dc.contributor.authorMa, VWS-
dc.contributor.authorCho, WCS-
dc.contributor.authorHo, YS-
dc.contributor.authorChang, RCC-
dc.date.accessioned2021-10-05T02:36:51Z-
dc.date.available2021-10-05T02:36:51Z-
dc.date.issued2021-
dc.identifier.citationNeuropathology and Applied Neurobiology, 2021, v. 47, p. 441-453-
dc.identifier.issn0305-1846-
dc.identifier.urihttp://hdl.handle.net/10722/304902-
dc.description.abstractAims: A variety of tissue clearing techniques have been developed to render intact tissue transparent. For thicker samples, additional partial tissue delipidation is re-quired before immersion into the final refractive index (RI)-matching solution, which alone is often inadequate to achieve full tissue transparency. However, it is difficult to determine a sufficient degree of tissue delipidation, excess of which can result in tissue distortion and protein loss. Here, we aim to develop a clearing strategy that allows better monitoring and more precise determination of delipidation progress.Methods: We combined the detergent sodium dodecyl sulphate (SDS) with OPTIClear, a RI-matching solution, to form a strategy termed Accurate delipidation with Optimal Clearing (Accu-OptiClearing). Accu-OptiClearing allows for a better preview of the final tissue transparency achieved when immersed in OPTIClear alone just before im-aging. We assessed for the changes in clearing rate, protein loss, degree of tissue dis-tortion, and preservation of antigens.Results: Partial delipidation using Accu-OptiClearing accelerated tissue clearing and better preserved tissue structure and antigens than delipidation with SDS alone. Despite achieving similar transparency in the final OPTIClear solution, more lipids were retained in samples cleared with Accu-OptiClearing compared to SDS.Conclusions: Combining the RI-matching solution OPTIClear with detergents, Accu-OptiClearing, can avoid excessive delipidation, leading to accelerated tissue clearing, less tissue damage and better preserved antigens.-
dc.languageeng-
dc.publisherWiley-Blackwell Publishing Ltd. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-2990-
dc.relation.ispartofNeuropathology and Applied Neurobiology-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectantigenicity-
dc.subjectdelipidation-
dc.subjectopacity-
dc.subjectprotein loss-
dc.subjectthree-dimensional imaging-
dc.titleOptimised tissue clearing minimises distortion and destruction during tissue delipidation-
dc.typeArticle-
dc.identifier.emailLai, HM: lhchem@hku.hk-
dc.identifier.emailChang, RCC: rccchang@hku.hk-
dc.identifier.authorityHui, ESK=rp01832-
dc.identifier.authorityChang, RCC=rp00470-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1111/nan.12673-
dc.identifier.pmid33107057-
dc.identifier.pmcidPMC8048831-
dc.identifier.scopuseid_2-s2.0-85096951561-
dc.identifier.hkuros326328-
dc.identifier.volume47-
dc.identifier.spage441-
dc.identifier.epage453-
dc.identifier.isiWOS:000595018800001-
dc.publisher.placeUnited Kingdom-

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