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Article: Derivation of Oligodendrocyte Precursors from Adult Bone Marrow Stromal Cells for Remyelination Therapy

TitleDerivation of Oligodendrocyte Precursors from Adult Bone Marrow Stromal Cells for Remyelination Therapy
Authors
Keywordscell therapy
oligodendrocyte precursors
bone marrow stromal cells
directed differentiation
myelin disorders
Issue Date2021
PublisherMDPI AG. The Journal's web site is located at http://www.mdpi.com/journal/cells
Citation
Cells, 2021, v. 10 n. 8, p. article no. 2166 How to Cite?
AbstractTransplantation of oligodendrocyte precursors (OPs) is potentially therapeutic for myelin disorders but a safe and accessible cell source remains to be identified. Here we report a two-step protocol for derivation of highly enriched populations of OPs from bone marrow stromal cells of young adult rats (aMSCs). Neural progenitors among the aMSCs were expanded in non-adherent sphere-forming cultures and subsequently directed along the OP lineage with the use of glial-inducing growth factors. Immunocytochemical and flow cytometric analyses of these cells confirmed OP-like expression of Olig2, PDGFRα, NG2, and Sox10. OPs so derived formed compact myelin both in vitro, as in co-culture with purified neurons, and in vivo, following transplantation into the corpus callosum of neonatal shiverer mice. Not only did the density of myelinated axons in the corpus callosum of recipient shiverer mice reach levels comparable to those in age-matched wild-type mice, but the mean lifespan of recipient shiverer mice also far exceeded those of non-recipient shiverer mice. Our results thus promise progress in harnessing the OP-generating potential of aMSCs towards cell therapy for myelin disorders.
Persistent Identifierhttp://hdl.handle.net/10722/304560
ISSN
2017 Impact Factor: 4.829
2020 SCImago Journal Rankings: 1.220
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorTsui, YP-
dc.contributor.authorLAM, G-
dc.contributor.authorWu, KLK-
dc.contributor.authorLi, MTS-
dc.contributor.authorTam, KW-
dc.contributor.authorShum, DKY-
dc.contributor.authorChan, YS-
dc.date.accessioned2021-09-23T09:01:48Z-
dc.date.available2021-09-23T09:01:48Z-
dc.date.issued2021-
dc.identifier.citationCells, 2021, v. 10 n. 8, p. article no. 2166-
dc.identifier.issn2073-4409-
dc.identifier.urihttp://hdl.handle.net/10722/304560-
dc.description.abstractTransplantation of oligodendrocyte precursors (OPs) is potentially therapeutic for myelin disorders but a safe and accessible cell source remains to be identified. Here we report a two-step protocol for derivation of highly enriched populations of OPs from bone marrow stromal cells of young adult rats (aMSCs). Neural progenitors among the aMSCs were expanded in non-adherent sphere-forming cultures and subsequently directed along the OP lineage with the use of glial-inducing growth factors. Immunocytochemical and flow cytometric analyses of these cells confirmed OP-like expression of Olig2, PDGFRα, NG2, and Sox10. OPs so derived formed compact myelin both in vitro, as in co-culture with purified neurons, and in vivo, following transplantation into the corpus callosum of neonatal shiverer mice. Not only did the density of myelinated axons in the corpus callosum of recipient shiverer mice reach levels comparable to those in age-matched wild-type mice, but the mean lifespan of recipient shiverer mice also far exceeded those of non-recipient shiverer mice. Our results thus promise progress in harnessing the OP-generating potential of aMSCs towards cell therapy for myelin disorders.-
dc.languageeng-
dc.publisherMDPI AG. The Journal's web site is located at http://www.mdpi.com/journal/cells-
dc.relation.ispartofCells-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectcell therapy-
dc.subjectoligodendrocyte precursors-
dc.subjectbone marrow stromal cells-
dc.subjectdirected differentiation-
dc.subjectmyelin disorders-
dc.titleDerivation of Oligodendrocyte Precursors from Adult Bone Marrow Stromal Cells for Remyelination Therapy-
dc.typeArticle-
dc.identifier.emailWu, KLK: lwu03@hku.hk-
dc.identifier.emailLi, MTS: tsliaa@connect.hku.hk-
dc.identifier.emailTam, KW: tamkw@hku.hk-
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hk-
dc.identifier.emailChan, YS: yschan@hku.hk-
dc.identifier.authorityShum, DKY=rp00321-
dc.identifier.authorityChan, YS=rp00318-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3390/cells10082166-
dc.identifier.pmid34440935-
dc.identifier.pmcidPMC8391516-
dc.identifier.scopuseid_2-s2.0-85115190776-
dc.identifier.hkuros325684-
dc.identifier.volume10-
dc.identifier.issue8-
dc.identifier.spagearticle no. 2166-
dc.identifier.epagearticle no. 2166-
dc.publisher.placeSwitzerland-

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