File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Isolation of MERS-related coronavirus from lesser bamboo bats that uses DPP4 and infects human-DPP4-transgenic mice

TitleIsolation of MERS-related coronavirus from lesser bamboo bats that uses DPP4 and infects human-DPP4-transgenic mice
Authors
Issue Date2021
PublisherNature Research: Fully open access journals. The Journal's web site is located at http://www.nature.com/ncomms/index.html
Citation
Nature Communications, 2021, v. 12 n. 1, article no. 216 How to Cite?
AbstractWhile a number of human coronaviruses are believed to be originated from ancestral viruses in bats, it remains unclear if bat coronaviruses are ready to cause direct bat-to-human transmission. Here, we report the isolation of a MERS-related coronavirus, Tylonycteris-bat-CoV-HKU4, from lesser bamboo bats. Tylonycteris-bat-CoV-HKU4 replicates efficiently in human colorectal adenocarcinoma and hepatocarcinoma cells with cytopathic effects, and can utilize human-dipeptidyl-peptidase-4 and dromedary camel-dipeptidyl-peptidase-4 as the receptors for cell entry. Flow cytometry, co-immunoprecipitation and surface plasmon resonance assays show that Tylonycteris-bat-CoV-HKU4-receptor-binding-domain can bind human-dipeptidyl-peptidase-4, dromedary camel-dipeptidyl-peptidase-4, and Tylonycteris pachypus-dipeptidyl-peptidase-4. Tylonycteris-bat-CoV-HKU4 can infect human-dipeptidyl-peptidase-4-transgenic mice by intranasal inoculation with self-limiting disease. Positive virus and inflammatory changes were detected in lungs and brains of infected mice, associated with suppression of antiviral cytokines and activation of proinflammatory cytokines and chemokines. The results suggest that MERS-related bat coronaviruses may overcome species barrier by utilizing dipeptidyl-peptidase-4 and potentially emerge in humans by direct bat-to-human transmission.
Persistent Identifierhttp://hdl.handle.net/10722/304269
ISSN
2020 Impact Factor: 14.919
2020 SCImago Journal Rankings: 5.559
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLau, SKP-
dc.contributor.authorFan, RYY-
dc.contributor.authorZhu, L-
dc.contributor.authorLi, KSM-
dc.contributor.authorWong, ACP-
dc.contributor.authorLuk, HKH-
dc.contributor.authorWong, EYM-
dc.contributor.authorLam, CSF-
dc.contributor.authorLo, GCS-
dc.contributor.authorFung, J-
dc.contributor.authorHe, Z-
dc.contributor.authorFok, FCH-
dc.contributor.authorAu Yeung, RKH-
dc.contributor.authorZhang, L-
dc.contributor.authorKok, KH-
dc.contributor.authorYuen, KY-
dc.contributor.authorWoo, PCY-
dc.date.accessioned2021-09-23T08:57:38Z-
dc.date.available2021-09-23T08:57:38Z-
dc.date.issued2021-
dc.identifier.citationNature Communications, 2021, v. 12 n. 1, article no. 216-
dc.identifier.issn2041-1723-
dc.identifier.urihttp://hdl.handle.net/10722/304269-
dc.description.abstractWhile a number of human coronaviruses are believed to be originated from ancestral viruses in bats, it remains unclear if bat coronaviruses are ready to cause direct bat-to-human transmission. Here, we report the isolation of a MERS-related coronavirus, Tylonycteris-bat-CoV-HKU4, from lesser bamboo bats. Tylonycteris-bat-CoV-HKU4 replicates efficiently in human colorectal adenocarcinoma and hepatocarcinoma cells with cytopathic effects, and can utilize human-dipeptidyl-peptidase-4 and dromedary camel-dipeptidyl-peptidase-4 as the receptors for cell entry. Flow cytometry, co-immunoprecipitation and surface plasmon resonance assays show that Tylonycteris-bat-CoV-HKU4-receptor-binding-domain can bind human-dipeptidyl-peptidase-4, dromedary camel-dipeptidyl-peptidase-4, and Tylonycteris pachypus-dipeptidyl-peptidase-4. Tylonycteris-bat-CoV-HKU4 can infect human-dipeptidyl-peptidase-4-transgenic mice by intranasal inoculation with self-limiting disease. Positive virus and inflammatory changes were detected in lungs and brains of infected mice, associated with suppression of antiviral cytokines and activation of proinflammatory cytokines and chemokines. The results suggest that MERS-related bat coronaviruses may overcome species barrier by utilizing dipeptidyl-peptidase-4 and potentially emerge in humans by direct bat-to-human transmission.-
dc.languageeng-
dc.publisherNature Research: Fully open access journals. The Journal's web site is located at http://www.nature.com/ncomms/index.html-
dc.relation.ispartofNature Communications-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleIsolation of MERS-related coronavirus from lesser bamboo bats that uses DPP4 and infects human-DPP4-transgenic mice-
dc.typeArticle-
dc.identifier.emailLi, KSM: kenn105@hkucc.hku.hk-
dc.identifier.emailLuk, HKH: hkhluk@hku.hk-
dc.identifier.emailAu Yeung, RKH: rex.auyeung@hku.hk-
dc.identifier.emailKok, KH: khkok@hku.hk-
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hk-
dc.identifier.authorityLau, SKP=rp00486-
dc.identifier.authorityAu Yeung, RKH=rp01877-
dc.identifier.authorityKok, KH=rp01455-
dc.identifier.authorityYuen, KY=rp00366-
dc.identifier.authorityWoo, PCY=rp00430-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1038/s41467-020-20458-9-
dc.identifier.pmid33431849-
dc.identifier.pmcidPMC7801609-
dc.identifier.scopuseid_2-s2.0-85099248484-
dc.identifier.hkuros325040-
dc.identifier.volume12-
dc.identifier.issue1-
dc.identifier.spagearticle no. 216-
dc.identifier.epagearticle no. 216-
dc.identifier.isiWOS:000670283600001-
dc.publisher.placeUnited Kingdom-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats