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Conference Paper: Poly(n-isopropyl acrylamide) coating of microchannels and bacterial sample loading via capillary-driven flow
Title | Poly(n-isopropyl acrylamide) coating of microchannels and bacterial sample loading via capillary-driven flow |
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Authors | |
Keywords | Bacteria Antimicrobial resistance Capillary-driven flow Microfluidics |
Issue Date | 2020 |
Citation | MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 2020, p. 174-175 How to Cite? |
Abstract | This paper showcases a capillary-driven flow microfluidics device (Chip-and-Dip) for loading of bacterial/pathogenic samples into microchannels for antimicrobial testing. This device offers the capability of capturing cells into microchannels that can be further treated with reagents to generate a colorimetric/fluorescent signal. The Chip-and-Dip device, fabricated with inexpensive materials and coated with these reagents, works by simply dipping the reagents-coated microfluidics chip into a sample. Here, we show a successful coating of microchannels with fluorescently labelled polymer and loading of Enterococcus Faecalis spiked in milk samples. |
Persistent Identifier | http://hdl.handle.net/10722/303720 |
DC Field | Value | Language |
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dc.contributor.author | Hassan, Sammer Ul | - |
dc.contributor.author | Carter, Steve | - |
dc.contributor.author | Singh, Sehaj | - |
dc.contributor.author | Dyson, Edward | - |
dc.contributor.author | Rimmer, Stephen | - |
dc.contributor.author | Zhang, Xunli | - |
dc.date.accessioned | 2021-09-15T08:25:53Z | - |
dc.date.available | 2021-09-15T08:25:53Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 2020, p. 174-175 | - |
dc.identifier.uri | http://hdl.handle.net/10722/303720 | - |
dc.description.abstract | This paper showcases a capillary-driven flow microfluidics device (Chip-and-Dip) for loading of bacterial/pathogenic samples into microchannels for antimicrobial testing. This device offers the capability of capturing cells into microchannels that can be further treated with reagents to generate a colorimetric/fluorescent signal. The Chip-and-Dip device, fabricated with inexpensive materials and coated with these reagents, works by simply dipping the reagents-coated microfluidics chip into a sample. Here, we show a successful coating of microchannels with fluorescently labelled polymer and loading of Enterococcus Faecalis spiked in milk samples. | - |
dc.language | eng | - |
dc.relation.ispartof | MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences | - |
dc.subject | Bacteria | - |
dc.subject | Antimicrobial resistance | - |
dc.subject | Capillary-driven flow | - |
dc.subject | Microfluidics | - |
dc.title | Poly(n-isopropyl acrylamide) coating of microchannels and bacterial sample loading via capillary-driven flow | - |
dc.type | Conference_Paper | - |
dc.identifier.scopus | eid_2-s2.0-85098245898 | - |
dc.identifier.spage | 174 | - |
dc.identifier.epage | 175 | - |