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- Publisher Website: 10.1016/j.msec.2021.112309
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Article: Spontaneously promoted osteogenic differentiation of MC3T3-E1 preosteoblasts on ultrathin layers of black phosphorus
Title | Spontaneously promoted osteogenic differentiation of MC3T3-E1 preosteoblasts on ultrathin layers of black phosphorus |
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Authors | |
Keywords | Black phosphorus Flow-enabled self-assembly Osteogenic differentiation Bone tissue engineering Biocompatibility |
Issue Date | 2021 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.journals.elsevier.com/materials-science-and-engineering-c |
Citation | Materials Science and Engineering: C, 2021, v. 128, p. article no. 112309 How to Cite? |
Abstract | Recently, black phosphorus (BP) has garnered great attention as one of newly emerging two-dimensional nanomaterials. Especially, the degraded platelets of BP in the physiological environment were shown to be nontoxic phosphate anions, which are a component of bone tissue and can be used for mineralization. Here, our study presents the potential of BP as biofunctional and biocompatible nanomaterials for the application to bone tissue engineering and regeneration. An ultrathin layer of BP nanodots (BPNDs) was created on a glass substrate by using a flow-enabled self-assembly process, which yielded a highly uniform deposition of BPNDs in a unique confined geometry. The BPND-coated substrates represented unprecedented favorable topographical microenvironments and supportive matrices suitable for the growth and survival of MC3T3-E1 preosteoblasts. The prepared substrates promoted the spontaneous osteodifferentiation of preosteoblasts, which had been confirmed by determining alkaline phosphatase activity and extracellular calcium deposition as early- and late-stage markers of osteogenic differentiation, respectively. Furthermore, the BPND-coated substrates upregulated the expression of some specific genes (i.e., RUNX2, OCN, OPN, and Vinculin) and proteins, which are closely related to osteogenesis. Conclusively, our BPND-coating strategy suggests that a biologically inert surface can be readily activated as a cell-favorable nanoplatform enabled with excellent biocompatibility and osteogenic ability. |
Persistent Identifier | http://hdl.handle.net/10722/302071 |
ISSN | 2023 Impact Factor: 8.1 2020 SCImago Journal Rankings: 1.234 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Jeon, S | - |
dc.contributor.author | Lee, JH | - |
dc.contributor.author | Jang, HJ | - |
dc.contributor.author | Lee, YB | - |
dc.contributor.author | Kim, B | - |
dc.contributor.author | Kang, MS | - |
dc.contributor.author | Shin, YC | - |
dc.contributor.author | Shin, DM | - |
dc.contributor.author | Hong, SW | - |
dc.contributor.author | Han, DW | - |
dc.date.accessioned | 2021-08-21T03:31:09Z | - |
dc.date.available | 2021-08-21T03:31:09Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Materials Science and Engineering: C, 2021, v. 128, p. article no. 112309 | - |
dc.identifier.issn | 0928-4931 | - |
dc.identifier.uri | http://hdl.handle.net/10722/302071 | - |
dc.description.abstract | Recently, black phosphorus (BP) has garnered great attention as one of newly emerging two-dimensional nanomaterials. Especially, the degraded platelets of BP in the physiological environment were shown to be nontoxic phosphate anions, which are a component of bone tissue and can be used for mineralization. Here, our study presents the potential of BP as biofunctional and biocompatible nanomaterials for the application to bone tissue engineering and regeneration. An ultrathin layer of BP nanodots (BPNDs) was created on a glass substrate by using a flow-enabled self-assembly process, which yielded a highly uniform deposition of BPNDs in a unique confined geometry. The BPND-coated substrates represented unprecedented favorable topographical microenvironments and supportive matrices suitable for the growth and survival of MC3T3-E1 preosteoblasts. The prepared substrates promoted the spontaneous osteodifferentiation of preosteoblasts, which had been confirmed by determining alkaline phosphatase activity and extracellular calcium deposition as early- and late-stage markers of osteogenic differentiation, respectively. Furthermore, the BPND-coated substrates upregulated the expression of some specific genes (i.e., RUNX2, OCN, OPN, and Vinculin) and proteins, which are closely related to osteogenesis. Conclusively, our BPND-coating strategy suggests that a biologically inert surface can be readily activated as a cell-favorable nanoplatform enabled with excellent biocompatibility and osteogenic ability. | - |
dc.language | eng | - |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.journals.elsevier.com/materials-science-and-engineering-c | - |
dc.relation.ispartof | Materials Science and Engineering: C | - |
dc.subject | Black phosphorus | - |
dc.subject | Flow-enabled self-assembly | - |
dc.subject | Osteogenic differentiation | - |
dc.subject | Bone tissue engineering | - |
dc.subject | Biocompatibility | - |
dc.title | Spontaneously promoted osteogenic differentiation of MC3T3-E1 preosteoblasts on ultrathin layers of black phosphorus | - |
dc.type | Article | - |
dc.identifier.email | Shin, DM: dmshin@hku.hk | - |
dc.identifier.authority | Shin, DM=rp02569 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.msec.2021.112309 | - |
dc.identifier.scopus | eid_2-s2.0-85110501935 | - |
dc.identifier.hkuros | 324368 | - |
dc.identifier.volume | 128 | - |
dc.identifier.spage | article no. 112309 | - |
dc.identifier.epage | article no. 112309 | - |
dc.identifier.isi | WOS:000691789300006 | - |
dc.publisher.place | Netherlands | - |