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Article: Interferon α2–Thymosin α1 Fusion Protein (IFNα2–Tα1): A Genetically Engineered Fusion Protein with Enhanced Anticancer and Antiviral Effect

TitleInterferon α2–Thymosin α1 Fusion Protein (IFNα2–Tα1): A Genetically Engineered Fusion Protein with Enhanced Anticancer and Antiviral Effect
Authors
Keywordsfusion protein
anti-proliferative effect
antiviral effect
genotoxic effect
relative expression
Issue Date2021
PublisherMDPIAG. The Journal's web site is located at http://www.mdpi.com/journal/materials/
Citation
Materials, 2021, v. 14 n. 12, article no. 3318 How to Cite?
AbstractHuman interferon α2 (IFNα2) and thymosin α1 (Tα1) are therapeutic proteins used for the treatment of viral infections and different types of cancer. Both IFNα2 and Tα1 show a synergic effect in their activities when used in combination. Furthermore, the therapeutic fusion proteins produced through the genetic fusion of two genes can exhibit several therapeutic functions in one molecule. In this study, we determined the anticancer and antiviral effect of human interferon α2–thymosin α1 fusion protein (IFNα2–Tα1) produced in our laboratory for the first time. The cytotoxic and genotoxic effect of IFNα2–Tα1 was evaluated in HepG2 and MDA-MB-231 cells. The in vitro assays confirmed that IFNα2–Tα1 inhibited the growth of cells more effectively than IFNα2 alone and showed an elevated genotoxic effect. The expression of proapoptotic genes was also significantly enhanced in IFNα2–Tα1-treated cells compared to IFNα2-treated cells. Furthermore, the HCV RNA level was significantly reduced in IFNα2–Tα1-treated HCV-infected Huh7 cells compared to IFNα2-treated cells. The quantitative PCR analysis showed that the expression of various genes, the products of which inhibit HCV replication, was significantly enhanced in IFNα2–Tα1-treated cells compared to IFNα2-treated cells. Our findings demonstrate that IFNα2–Tα1 is more effective than single IFNα2 as an anticancer and antiviral agent.
Persistent Identifierhttp://hdl.handle.net/10722/301949
ISSN
2023 Impact Factor: 3.1
2023 SCImago Journal Rankings: 0.565
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorAslam, MS-
dc.contributor.authorZaidi, SZJ-
dc.contributor.authorToor, RH-
dc.contributor.authorGull, I-
dc.contributor.authorIqbal, MM-
dc.contributor.authorAbbas, Z-
dc.contributor.authorTipu, I-
dc.contributor.authorAhmed, A-
dc.contributor.authorAthar, MA-
dc.contributor.authorHarito, C-
dc.contributor.authorHassan, SU-
dc.date.accessioned2021-08-21T03:29:20Z-
dc.date.available2021-08-21T03:29:20Z-
dc.date.issued2021-
dc.identifier.citationMaterials, 2021, v. 14 n. 12, article no. 3318-
dc.identifier.issn1996-1944-
dc.identifier.urihttp://hdl.handle.net/10722/301949-
dc.description.abstractHuman interferon α2 (IFNα2) and thymosin α1 (Tα1) are therapeutic proteins used for the treatment of viral infections and different types of cancer. Both IFNα2 and Tα1 show a synergic effect in their activities when used in combination. Furthermore, the therapeutic fusion proteins produced through the genetic fusion of two genes can exhibit several therapeutic functions in one molecule. In this study, we determined the anticancer and antiviral effect of human interferon α2–thymosin α1 fusion protein (IFNα2–Tα1) produced in our laboratory for the first time. The cytotoxic and genotoxic effect of IFNα2–Tα1 was evaluated in HepG2 and MDA-MB-231 cells. The in vitro assays confirmed that IFNα2–Tα1 inhibited the growth of cells more effectively than IFNα2 alone and showed an elevated genotoxic effect. The expression of proapoptotic genes was also significantly enhanced in IFNα2–Tα1-treated cells compared to IFNα2-treated cells. Furthermore, the HCV RNA level was significantly reduced in IFNα2–Tα1-treated HCV-infected Huh7 cells compared to IFNα2-treated cells. The quantitative PCR analysis showed that the expression of various genes, the products of which inhibit HCV replication, was significantly enhanced in IFNα2–Tα1-treated cells compared to IFNα2-treated cells. Our findings demonstrate that IFNα2–Tα1 is more effective than single IFNα2 as an anticancer and antiviral agent.-
dc.languageeng-
dc.publisherMDPIAG. The Journal's web site is located at http://www.mdpi.com/journal/materials/-
dc.relation.ispartofMaterials-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectfusion protein-
dc.subjectanti-proliferative effect-
dc.subjectantiviral effect-
dc.subjectgenotoxic effect-
dc.subjectrelative expression-
dc.titleInterferon α2–Thymosin α1 Fusion Protein (IFNα2–Tα1): A Genetically Engineered Fusion Protein with Enhanced Anticancer and Antiviral Effect-
dc.typeArticle-
dc.identifier.emailHassan, SU: suhassan@hku.hk-
dc.identifier.authorityHassan, SU=rp02857-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3390/ma14123318-
dc.identifier.pmid34203928-
dc.identifier.pmcidPMC8232609-
dc.identifier.scopuseid_2-s2.0-85108825218-
dc.identifier.hkuros324376-
dc.identifier.volume14-
dc.identifier.issue12-
dc.identifier.spagearticle no. 3318-
dc.identifier.epagearticle no. 3318-
dc.identifier.isiWOS:000666418100001-
dc.publisher.placeSwitzerland-

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