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Article: F4-Neuroprostanes: A Role in Sperm Capacitation

TitleF4-Neuroprostanes: A Role in Sperm Capacitation
Authors
Keywordscapacitation
CASA
human sperm motility
F4-neuroprostanes
Phospho-AMPKα
Issue Date2021
PublisherMDPI AG. The Journal's web site is located at https://www.mdpi.com/journal/life
Citation
Life, 2021, v. 11 n. 7, p. article no. 655 How to Cite?
AbstractF4-neuroprostanes (F4-NeuroPs), derived from the oxidative metabolization of docosahexaenoic acid (DHA), are considered biomarkers of oxidative stress in neurodegenerative diseases. Neurons and spermatozoa display a high DHA content. NeuroPs might possess biological activities. The aim of this in vitro study was to investigate the biological effects of chemically synthetized 4-F4t-NeuroP and 10-F4t-NeuroP in human sperm. Total progressive sperm motility (p < 0.05) and linearity (p = 0.016), evaluated by a computer-assisted sperm analyzer, were significantly increased in samples incubated with 7 ng F4-NeuroPs compared to non-supplemented controls. Sperm capacitation was tested in rabbit and swim-up-selected human sperm by chlortetracycline fluorescence assay. A higher percentage of capacitated sperm (p < 0.01) was observed in samples incubated in F4-NeuroPs than in the controls. However, the percentage of capacitated sperm was not different in F4-NeuroPs and calcium ionophore treatments at 2 h incubation. The phosphorylated form of AMPKα was detected by immunofluorescence analysis; after 2 h F4-NeuroP incubation, a dotted signal appeared in the entire sperm tail, and in controls, sperm were labeled in the mid-piece. A defined level of seminal F4-NeuroPs (7 ng) showed a biological activity in sperm function; its addition in sperm suspensions stimulated capacitation, increasing the number of sperm able to fertilize.
Persistent Identifierhttp://hdl.handle.net/10722/301201
ISSN
2020 SCImago Journal Rankings: 0.973
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSignorini, C-
dc.contributor.authorMoretti, E-
dc.contributor.authorNoto, D-
dc.contributor.authorMattioli, S-
dc.contributor.authorCastellini, C-
dc.contributor.authorPascarelli, NA-
dc.contributor.authorDurand, T-
dc.contributor.authorOger, C-
dc.contributor.authorGalano, JM-
dc.contributor.authorDe Felice, C-
dc.contributor.authorLee, JCY-
dc.contributor.authorCollodel, G-
dc.date.accessioned2021-07-27T08:07:37Z-
dc.date.available2021-07-27T08:07:37Z-
dc.date.issued2021-
dc.identifier.citationLife, 2021, v. 11 n. 7, p. article no. 655-
dc.identifier.issn2075-1729-
dc.identifier.urihttp://hdl.handle.net/10722/301201-
dc.description.abstractF4-neuroprostanes (F4-NeuroPs), derived from the oxidative metabolization of docosahexaenoic acid (DHA), are considered biomarkers of oxidative stress in neurodegenerative diseases. Neurons and spermatozoa display a high DHA content. NeuroPs might possess biological activities. The aim of this in vitro study was to investigate the biological effects of chemically synthetized 4-F4t-NeuroP and 10-F4t-NeuroP in human sperm. Total progressive sperm motility (p < 0.05) and linearity (p = 0.016), evaluated by a computer-assisted sperm analyzer, were significantly increased in samples incubated with 7 ng F4-NeuroPs compared to non-supplemented controls. Sperm capacitation was tested in rabbit and swim-up-selected human sperm by chlortetracycline fluorescence assay. A higher percentage of capacitated sperm (p < 0.01) was observed in samples incubated in F4-NeuroPs than in the controls. However, the percentage of capacitated sperm was not different in F4-NeuroPs and calcium ionophore treatments at 2 h incubation. The phosphorylated form of AMPKα was detected by immunofluorescence analysis; after 2 h F4-NeuroP incubation, a dotted signal appeared in the entire sperm tail, and in controls, sperm were labeled in the mid-piece. A defined level of seminal F4-NeuroPs (7 ng) showed a biological activity in sperm function; its addition in sperm suspensions stimulated capacitation, increasing the number of sperm able to fertilize.-
dc.languageeng-
dc.publisherMDPI AG. The Journal's web site is located at https://www.mdpi.com/journal/life-
dc.relation.ispartofLife-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectcapacitation-
dc.subjectCASA-
dc.subjecthuman sperm motility-
dc.subjectF4-neuroprostanes-
dc.subjectPhospho-AMPKα-
dc.titleF4-Neuroprostanes: A Role in Sperm Capacitation-
dc.typeArticle-
dc.identifier.emailLee, JCY: jettylee@hku.hk-
dc.identifier.authorityLee, JCY=rp01511-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3390/life11070655-
dc.identifier.scopuseid_2-s2.0-85111847737-
dc.identifier.hkuros323406-
dc.identifier.volume11-
dc.identifier.issue7-
dc.identifier.spagearticle no. 655-
dc.identifier.epagearticle no. 655-
dc.identifier.isiWOS:000676813500001-
dc.publisher.placeSwitzerland-

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