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Article: Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

TitleGuidelines for the use and interpretation of assays for monitoring autophagy (4th edition)
Authors
KeywordsAutophagosome
cancer
flux
LC3
lysosome
Issue Date2021
PublisherTaylor & Francis: STM, Behavioural Science and Public Health Titles. The Journal's web site is located at http://www.tandfonline.com/loi/kaup20#.VfwhQUaFOnI
Citation
Autophagy, 2021, v. 17 n. 1, p. 1-382 How to Cite?
AbstractIn 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.
DescriptionBronze open access
Persistent Identifierhttp://hdl.handle.net/10722/299794
ISSN
2023 Impact Factor: 14.6
2023 SCImago Journal Rankings: 4.035
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorKlionsky, DJ-
dc.contributor.authorChan, DW-
dc.contributor.authorChan, YS-
dc.date.accessioned2021-05-26T03:29:09Z-
dc.date.available2021-05-26T03:29:09Z-
dc.date.issued2021-
dc.identifier.citationAutophagy, 2021, v. 17 n. 1, p. 1-382-
dc.identifier.issn1554-8627-
dc.identifier.urihttp://hdl.handle.net/10722/299794-
dc.descriptionBronze open access-
dc.description.abstractIn 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.-
dc.languageeng-
dc.publisherTaylor & Francis: STM, Behavioural Science and Public Health Titles. The Journal's web site is located at http://www.tandfonline.com/loi/kaup20#.VfwhQUaFOnI-
dc.relation.ispartofAutophagy-
dc.rightsAccepted Manuscript (AM) i.e. Postprint This is an Accepted Manuscript of an article published by Taylor & Francis in [JOURNAL TITLE] on [date of publication], available online: http://www.tandfonline.com/[Article DOI].-
dc.subjectAutophagosome-
dc.subjectcancer-
dc.subjectflux-
dc.subjectLC3-
dc.subjectlysosome-
dc.titleGuidelines for the use and interpretation of assays for monitoring autophagy (4th edition)-
dc.typeArticle-
dc.identifier.emailChan, DW: dwchan@hku.hk-
dc.identifier.authorityChan, DW=rp00543-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1080/15548627.2020.1797280-
dc.identifier.pmid33634751-
dc.identifier.pmcidPMC7996087-
dc.identifier.scopuseid_2-s2.0-85102619204-
dc.identifier.hkuros322589-
dc.identifier.volume17-
dc.identifier.issue1-
dc.identifier.spage1-
dc.identifier.epage382-
dc.identifier.isiWOS:000636121800001-
dc.publisher.placeUnited States-

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