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Article: Granzyme B-expressing γδ-T and NK cells as a predictor of clinical pregnancy failure in patients with unexplained repeated implantation failure

TitleGranzyme B-expressing γδ-T and NK cells as a predictor of clinical pregnancy failure in patients with unexplained repeated implantation failure
Authors
Keywordsγδ-T cells
Cytotoxic granules
Granzyme B
Repeated implantation failure
Clinical pregnancy
Issue Date2021
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jri
Citation
Journal of Reproductive Immunology, 2021, v. 144, p. article no. 103269 How to Cite?
AbstractThe limited cytotoxicity of immune cells facilitates a successful establishment of pregnancy. However, the association between cytotoxic granules and unexplained repeated implantation failure (uRIF) remains unkown. Twenty-one fertile controls and 54 patients with uRIF were included in this study. The pregnancy outcomes were monitored at different gestational periods. The peripheral blood lymphocytes were detected using specific monoclonal antibodies by flow cytometry. The percentage of perforin+ (Pfr+), granzyme B+ (GrB+), or granulysin+ (Gnly+) lymphocytes was not significantly different among fertile controls, uRIF patients with successful pregnancy outcomes, and uRIF patients with pregnancy failure. The percentage of GrB+ γδ-T cells in lymphocytes was markedly higher in uRIF patients with implantation failure and clinical pregnancy failure than that in uRIF patients with a corresponding successful pregnancy outcome. A four-tier risk model showed that the risk of suffering clinical pregnancy failure in uRIF patients among high risk tier (83.3 %), normal risk tier (65.0 %) and low risk tier (39.1 %) was elevated by 2–4 fold compared with uRIF patients among lowest risk tier (20.0 %). In addition, the percentage of GrB+ NK cells in lymphocytes tended to decrease in uRIF patients with pregnancy failure. The AUC of the combined indicator with GrB+ γδ-T cells and GrB+ NK cells was increased than that of GrB+ γδ-T cells and GrB+ NK cells for predicting clinical pregnancy failure. In conclusion, the frequency of GrB-expressing γδ-T and NK cells in peripheral blood could serve as a predictor of clinical pregnancy failure in patients with uRIF.
Persistent Identifierhttp://hdl.handle.net/10722/299772
ISSN
2023 Impact Factor: 2.9
2023 SCImago Journal Rankings: 0.915
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHUANG, C-
dc.contributor.authorZhang, Y-
dc.contributor.authorXiang, Z-
dc.contributor.authorLi, Y-
dc.contributor.authorLin, R-
dc.contributor.authorXu, J-
dc.contributor.authorTu, W-
dc.contributor.authorZeng, Y-
dc.date.accessioned2021-05-26T03:28:50Z-
dc.date.available2021-05-26T03:28:50Z-
dc.date.issued2021-
dc.identifier.citationJournal of Reproductive Immunology, 2021, v. 144, p. article no. 103269-
dc.identifier.issn0165-0378-
dc.identifier.urihttp://hdl.handle.net/10722/299772-
dc.description.abstractThe limited cytotoxicity of immune cells facilitates a successful establishment of pregnancy. However, the association between cytotoxic granules and unexplained repeated implantation failure (uRIF) remains unkown. Twenty-one fertile controls and 54 patients with uRIF were included in this study. The pregnancy outcomes were monitored at different gestational periods. The peripheral blood lymphocytes were detected using specific monoclonal antibodies by flow cytometry. The percentage of perforin+ (Pfr+), granzyme B+ (GrB+), or granulysin+ (Gnly+) lymphocytes was not significantly different among fertile controls, uRIF patients with successful pregnancy outcomes, and uRIF patients with pregnancy failure. The percentage of GrB+ γδ-T cells in lymphocytes was markedly higher in uRIF patients with implantation failure and clinical pregnancy failure than that in uRIF patients with a corresponding successful pregnancy outcome. A four-tier risk model showed that the risk of suffering clinical pregnancy failure in uRIF patients among high risk tier (83.3 %), normal risk tier (65.0 %) and low risk tier (39.1 %) was elevated by 2–4 fold compared with uRIF patients among lowest risk tier (20.0 %). In addition, the percentage of GrB+ NK cells in lymphocytes tended to decrease in uRIF patients with pregnancy failure. The AUC of the combined indicator with GrB+ γδ-T cells and GrB+ NK cells was increased than that of GrB+ γδ-T cells and GrB+ NK cells for predicting clinical pregnancy failure. In conclusion, the frequency of GrB-expressing γδ-T and NK cells in peripheral blood could serve as a predictor of clinical pregnancy failure in patients with uRIF.-
dc.languageeng-
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jri-
dc.relation.ispartofJournal of Reproductive Immunology-
dc.subjectγδ-T cells-
dc.subjectCytotoxic granules-
dc.subjectGranzyme B-
dc.subjectRepeated implantation failure-
dc.subjectClinical pregnancy-
dc.titleGranzyme B-expressing γδ-T and NK cells as a predictor of clinical pregnancy failure in patients with unexplained repeated implantation failure-
dc.typeArticle-
dc.identifier.emailTu, W: wwtu@hku.hk-
dc.identifier.authorityTu, W=rp00416-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jri.2020.103269-
dc.identifier.pmid33540297-
dc.identifier.scopuseid_2-s2.0-85100103305-
dc.identifier.hkuros322492-
dc.identifier.volume144-
dc.identifier.spagearticle no. 103269-
dc.identifier.epagearticle no. 103269-
dc.identifier.isiWOS:000624565500011-
dc.publisher.placeIreland-

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