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Article: A sensitive and specific competitive enzyme-linked immunosorbent assay for serodiagnosis of COVID-19 in animals

TitleA sensitive and specific competitive enzyme-linked immunosorbent assay for serodiagnosis of COVID-19 in animals
Authors
KeywordsSARS-CoV-2
nucleocapsid protein
competitive ELISA
antibody
COVID-19
Issue Date2021
PublisherMDPI AG. The Journal's web site is located at http://www.mdpi.com/journal/microorganisms
Citation
Microorganisms, 2021, v. 9, p. article no. 1019 How to Cite?
AbstractIn addition to human cases, cases of COVID-19 in captive animals and pets are increasingly reported. This raises the concern for two-way COVID-19 transmission between humans and animals. Here, we developed a SARS-CoV-2 nucleocapsid protein-based competitive enzyme-linked immunosorbent assay (cELISA) for serodiagnosis of COVID-19 which can theoretically be used in virtually all kinds of animals. We used 187 serum samples from patients with/without COVID-19, laboratory animals immunized with inactive SARS-CoV-2 virions, COVID-19-negative animals, and animals seropositive to other betacoronaviruses. A cut-off percent inhibition value of 22.345% was determined and the analytical sensitivity and specificity were found to be 1:64–1:256 and 93.9%, respectively. Evaluation on its diagnostic performance using 155 serum samples from COVID-19-negative animals and COVID-19 human patients showed a diagnostic sensitivity and specificity of 80.8% and 100%, respectively. The cELISA can be incorporated into routine blood testing of farmed/captive animals for COVID-19 surveillance.
Persistent Identifierhttp://hdl.handle.net/10722/299731
ISSN
2018 Impact Factor: 4.167
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorLau, SKP-
dc.contributor.authorHE, Z-
dc.contributor.authorTsang, CC-
dc.contributor.authorCHAN, TTY-
dc.contributor.authorLuk, HKH-
dc.contributor.authorChan, E-
dc.contributor.authorLi, KSM-
dc.contributor.authorFUNG, J-
dc.contributor.authorChow, FWN-
dc.contributor.authorTam, AR-
dc.contributor.authorChung, TWH-
dc.contributor.authorWong, SCY-
dc.contributor.authorQue, TL-
dc.contributor.authorFung, KSC-
dc.contributor.authorLung, DC-
dc.contributor.authorWu, AKL-
dc.contributor.authorHung, IFN-
dc.contributor.authorTeng, JLL-
dc.contributor.authorWernery, U-
dc.contributor.authorHui, SW-
dc.contributor.authorMartelli, P-
dc.contributor.authorWoo, PCY-
dc.date.accessioned2021-05-26T03:28:16Z-
dc.date.available2021-05-26T03:28:16Z-
dc.date.issued2021-
dc.identifier.citationMicroorganisms, 2021, v. 9, p. article no. 1019-
dc.identifier.issn2076-2607-
dc.identifier.urihttp://hdl.handle.net/10722/299731-
dc.description.abstractIn addition to human cases, cases of COVID-19 in captive animals and pets are increasingly reported. This raises the concern for two-way COVID-19 transmission between humans and animals. Here, we developed a SARS-CoV-2 nucleocapsid protein-based competitive enzyme-linked immunosorbent assay (cELISA) for serodiagnosis of COVID-19 which can theoretically be used in virtually all kinds of animals. We used 187 serum samples from patients with/without COVID-19, laboratory animals immunized with inactive SARS-CoV-2 virions, COVID-19-negative animals, and animals seropositive to other betacoronaviruses. A cut-off percent inhibition value of 22.345% was determined and the analytical sensitivity and specificity were found to be 1:64–1:256 and 93.9%, respectively. Evaluation on its diagnostic performance using 155 serum samples from COVID-19-negative animals and COVID-19 human patients showed a diagnostic sensitivity and specificity of 80.8% and 100%, respectively. The cELISA can be incorporated into routine blood testing of farmed/captive animals for COVID-19 surveillance.-
dc.languageeng-
dc.publisherMDPI AG. The Journal's web site is located at http://www.mdpi.com/journal/microorganisms-
dc.relation.ispartofMicroorganisms-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectSARS-CoV-2-
dc.subjectnucleocapsid protein-
dc.subjectcompetitive ELISA-
dc.subjectantibody-
dc.subjectCOVID-19-
dc.titleA sensitive and specific competitive enzyme-linked immunosorbent assay for serodiagnosis of COVID-19 in animals-
dc.typeArticle-
dc.identifier.emailTsang, CC: microbioct@connect.hku.hk-
dc.identifier.emailLuk, HKH: hkhluk@hku.hk-
dc.identifier.emailChan, E: echan206@hku.hk-
dc.identifier.emailLi, KSM: kenn105@hkucc.hku.hk-
dc.identifier.emailChow, FWN: chow5810@hku.hk-
dc.identifier.emailWong, SCY: wcy288@HKUCC-COM.hku.hk-
dc.identifier.emailWu, AKL: alanklwu@hkucc.hku.hk-
dc.identifier.emailHung, IFN: ivanhung@hkucc.hku.hk-
dc.identifier.emailTeng, JLL: llteng@hku.hk-
dc.identifier.authorityLau, SKP=rp00486-
dc.identifier.authorityTsang, CC=rp02492-
dc.identifier.authorityChow, FWN=rp02493-
dc.identifier.authorityHung, IFN=rp00508-
dc.identifier.authorityTeng, JLL=rp00277-
dc.identifier.authorityWoo, PCY=rp00430-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3390/microorganisms9051019-
dc.identifier.pmid34068581-
dc.identifier.pmcidPMC8150753-
dc.identifier.scopuseid_2-s2.0-85105537925-
dc.identifier.hkuros322486-
dc.identifier.volume9-
dc.identifier.spagearticle no. 1019-
dc.identifier.epagearticle no. 1019-
dc.publisher.placeSwitzerland-

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