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- Publisher Website: 10.1073/pnas.2017645118
- Scopus: eid_2-s2.0-85105529366
- PMID: 33947811
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Article: PI(3,4)P2-mediated membrane tubulation promotes integrin trafficking and invasive cell migration
Title | PI(3,4)P2-mediated membrane tubulation promotes integrin trafficking and invasive cell migration |
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Authors | |
Keywords | phosphoinositide lipids invadopodia integrin trafficking microtubule |
Issue Date | 2021 |
Publisher | National Academy of Sciences. The Journal's web site is located at http://www.pnas.org |
Citation | Proceedings of the National Academy of Sciences, 2021, v. 118 n. 19, p. article no. e2017645118 How to Cite? |
Abstract | Invadopodia are integrin-mediated adhesions with abundant PI(3,4)P2. However, the functional role of PI(3,4)P2 in adhesion signaling remains unclear. Here, we find that the PI(3,4)P2 biogenesis regulates the integrin endocytosis at invadopodia. PI(3,4)P2 is locally produced by PIK3CA and SHIP2 and is concentrated at the trailing edge of the invadopodium arc. The PI(3,4)P2-rich compartment locally forms small puncta (membrane buds) in a SNX9-dependent manner, recruits dynein activator Hook1 through AKTIP, and rearranges into micrometer-long tubular invaginations (membrane tubes). The uncurving membrane tube extends rapidly, follows the retrograde movement of dynein along microtubule tracks, and disconnects from the plasma membrane. Activated integrin-beta3 is locally internalized through the pathway of PI(3,4)P2-mediated membrane invagination and is then actively recycled. Blockages of PI3K, SHIP2, and SNX9 suppress integrin-beta3 endocytosis, delay adhesion turnover, and impede transwell invasion of MEF-Src and MDA-MB-231 cells. Thus, the production of PI(3,4)P2 promotes invasive cell migration by stimulating the trafficking of integrin receptor at the invadopodium. |
Description | Hybrid open access |
Persistent Identifier | http://hdl.handle.net/10722/299690 |
ISSN | 2023 Impact Factor: 9.4 2023 SCImago Journal Rankings: 3.737 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Feng, Z | - |
dc.contributor.author | Yu, CH | - |
dc.date.accessioned | 2021-05-26T03:27:41Z | - |
dc.date.available | 2021-05-26T03:27:41Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Proceedings of the National Academy of Sciences, 2021, v. 118 n. 19, p. article no. e2017645118 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | http://hdl.handle.net/10722/299690 | - |
dc.description | Hybrid open access | - |
dc.description.abstract | Invadopodia are integrin-mediated adhesions with abundant PI(3,4)P2. However, the functional role of PI(3,4)P2 in adhesion signaling remains unclear. Here, we find that the PI(3,4)P2 biogenesis regulates the integrin endocytosis at invadopodia. PI(3,4)P2 is locally produced by PIK3CA and SHIP2 and is concentrated at the trailing edge of the invadopodium arc. The PI(3,4)P2-rich compartment locally forms small puncta (membrane buds) in a SNX9-dependent manner, recruits dynein activator Hook1 through AKTIP, and rearranges into micrometer-long tubular invaginations (membrane tubes). The uncurving membrane tube extends rapidly, follows the retrograde movement of dynein along microtubule tracks, and disconnects from the plasma membrane. Activated integrin-beta3 is locally internalized through the pathway of PI(3,4)P2-mediated membrane invagination and is then actively recycled. Blockages of PI3K, SHIP2, and SNX9 suppress integrin-beta3 endocytosis, delay adhesion turnover, and impede transwell invasion of MEF-Src and MDA-MB-231 cells. Thus, the production of PI(3,4)P2 promotes invasive cell migration by stimulating the trafficking of integrin receptor at the invadopodium. | - |
dc.language | eng | - |
dc.publisher | National Academy of Sciences. The Journal's web site is located at http://www.pnas.org | - |
dc.relation.ispartof | Proceedings of the National Academy of Sciences | - |
dc.rights | Proceedings of the National Academy of Sciences. Copyright © National Academy of Sciences. | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | phosphoinositide lipids | - |
dc.subject | invadopodia | - |
dc.subject | integrin trafficking | - |
dc.subject | microtubule | - |
dc.title | PI(3,4)P2-mediated membrane tubulation promotes integrin trafficking and invasive cell migration | - |
dc.type | Article | - |
dc.identifier.email | Yu, CH: chyu1@hku.hk | - |
dc.identifier.authority | Yu, CH=rp01930 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1073/pnas.2017645118 | - |
dc.identifier.pmid | 33947811 | - |
dc.identifier.pmcid | PMC8126793 | - |
dc.identifier.scopus | eid_2-s2.0-85105529366 | - |
dc.identifier.hkuros | 322581 | - |
dc.identifier.volume | 118 | - |
dc.identifier.issue | 19 | - |
dc.identifier.spage | article no. e2017645118 | - |
dc.identifier.epage | article no. e2017645118 | - |
dc.identifier.isi | WOS:000651329300002 | - |
dc.publisher.place | United States | - |