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- Publisher Website: 10.1016/j.cell.2019.08.019
- Scopus: eid_2-s2.0-85071335522
- PMID: 31491385
- WOS: WOS:000483983000015
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Article: Amyloid-like Assembly Activates a Phosphatase in the Developing Drosophila Embryo
Title | Amyloid-like Assembly Activates a Phosphatase in the Developing Drosophila Embryo |
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Authors | |
Keywords | phosphatase Herzog CG5830 patterning Drosophila melanogaster prion-like protein segment polarity amyloid Hzg embryo |
Issue Date | 2019 |
Citation | Cell, 2019, v. 178, n. 6, p. 1403-1420.e21 How to Cite? |
Abstract | Prion-like proteins can assume distinct conformational and physical states in the same cell. Sequence analysis suggests that prion-like proteins are prevalent in various species; however, it remains unclear what functional space they occupy in multicellular organisms. Here, we report the identification of a prion-like protein, Herzog (CG5830), through a multimodal screen in Drosophila melanogaster. Herzog functions as a membrane-associated phosphatase and controls embryonic patterning, likely being involved in TGF-β/BMP and FGF/EGF signaling pathways. Remarkably, monomeric Herzog is enzymatically inactive and becomes active upon amyloid-like assembly. The prion-like domain of Herzog is necessary for both its assembly and membrane targeting. Removal of the prion-like domain impairs activity, while restoring assembly on the membrane using a heterologous prion-like domain and membrane-targeting motif can restore phosphatase activity. This study provides an example of a prion-like domain that allows an enzyme to gain essential functionality via amyloid-like assembly to control animal development. |
Persistent Identifier | http://hdl.handle.net/10722/299597 |
ISSN | 2023 Impact Factor: 45.5 2023 SCImago Journal Rankings: 24.342 |
ISI Accession Number ID | |
Errata |
DC Field | Value | Language |
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dc.contributor.author | Nil, Zelha | - |
dc.contributor.author | Hervás, Rubén | - |
dc.contributor.author | Gerbich, Therese | - |
dc.contributor.author | Leal, Paulo | - |
dc.contributor.author | Yu, Zulin | - |
dc.contributor.author | Saraf, Anita | - |
dc.contributor.author | Sardiu, Mihaela | - |
dc.contributor.author | Lange, Jeffrey J. | - |
dc.contributor.author | Yi, Kexi | - |
dc.contributor.author | Unruh, Jay | - |
dc.contributor.author | Slaughter, Brian | - |
dc.contributor.author | Si, Kausik | - |
dc.date.accessioned | 2021-05-21T03:34:45Z | - |
dc.date.available | 2021-05-21T03:34:45Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Cell, 2019, v. 178, n. 6, p. 1403-1420.e21 | - |
dc.identifier.issn | 0092-8674 | - |
dc.identifier.uri | http://hdl.handle.net/10722/299597 | - |
dc.description.abstract | Prion-like proteins can assume distinct conformational and physical states in the same cell. Sequence analysis suggests that prion-like proteins are prevalent in various species; however, it remains unclear what functional space they occupy in multicellular organisms. Here, we report the identification of a prion-like protein, Herzog (CG5830), through a multimodal screen in Drosophila melanogaster. Herzog functions as a membrane-associated phosphatase and controls embryonic patterning, likely being involved in TGF-β/BMP and FGF/EGF signaling pathways. Remarkably, monomeric Herzog is enzymatically inactive and becomes active upon amyloid-like assembly. The prion-like domain of Herzog is necessary for both its assembly and membrane targeting. Removal of the prion-like domain impairs activity, while restoring assembly on the membrane using a heterologous prion-like domain and membrane-targeting motif can restore phosphatase activity. This study provides an example of a prion-like domain that allows an enzyme to gain essential functionality via amyloid-like assembly to control animal development. | - |
dc.language | eng | - |
dc.relation.ispartof | Cell | - |
dc.subject | phosphatase | - |
dc.subject | Herzog | - |
dc.subject | CG5830 | - |
dc.subject | patterning | - |
dc.subject | Drosophila melanogaster | - |
dc.subject | prion-like protein | - |
dc.subject | segment polarity | - |
dc.subject | amyloid | - |
dc.subject | Hzg | - |
dc.subject | embryo | - |
dc.title | Amyloid-like Assembly Activates a Phosphatase in the Developing Drosophila Embryo | - |
dc.type | Article | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1016/j.cell.2019.08.019 | - |
dc.identifier.pmid | 31491385 | - |
dc.identifier.scopus | eid_2-s2.0-85071335522 | - |
dc.identifier.volume | 178 | - |
dc.identifier.issue | 6 | - |
dc.identifier.spage | 1403 | - |
dc.identifier.epage | 1420.e21 | - |
dc.identifier.eissn | 1097-4172 | - |
dc.identifier.isi | WOS:000483983000015 | - |
dc.relation.erratum | doi:10.1016/j.cell.2019.09.033 | - |
dc.relation.erratum | eid:eid_2-s2.0-85072998798 | - |