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- Publisher Website: 10.1016/j.joen.2020.08.026
- Scopus: eid_2-s2.0-85096542827
- PMID: 33045266
- WOS: WOS:000602701600008
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Article: Fabrication of tapered fluidic microchannels conducive to angiogenic sprouting within gelatin methacryloyl hydrogels
Title | Fabrication of tapered fluidic microchannels conducive to angiogenic sprouting within gelatin methacryloyl hydrogels |
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Authors | |
Keywords | Angiogenic sprouting fluidic microchannels GelMA hydrogel prevascularization |
Issue Date | 2021 |
Publisher | Elsevier Inc. The Journal's web site is located at http://www.jendodon.com |
Citation | Journal of Endodontics, 2021, v. 47 n. 1, p. 52-61 How to Cite? |
Abstract | Introduction:
The transplantation of stem cells/tissue constructs into root canal space is a promising strategy for regenerating lost pulp tissue. However, the root canal system, which is cone shaped with a taper from the larger coronal end to the smaller apical end, limits the vascular supply and, therefore, the regenerative capacity. The current study aimed to fabricate built-in microchannels with different tapers to explore various approaches to endothelialize these microchannels.
Methods:
The fluidic microchannels with varying tapers (parallel, 0.04, and 0.06) were fabricated within gelatin methacryloyl (GelMA) hydrogel (with or without stem cell from the apical papilla [SCAP] encapsulation) of different concentrations (5%, 7.5%, and 10% [w/v]). Green fluorescent protein–expressing human umbilical vein endothelial cells (HUVECs-GFP) were seeded alone or with SCAPs in coculture into these microchannels. Angiogenic sprouting was assessed by fluorescence and a confocal microscope and ImageJ software (National Institutes of Health, Bethesda, MD). Immunostaining was conducted to illustrate monolayer formation. Data were statistically analyzed by 1-way/2-way analysis of variance.
Results:
HUVEC-only inoculation formed an endothelial monolayer inside the microchannel without angiogenic sprouting. HUVECs-GFP/SCAPs cocultured at a 1:1 ratio produced the longest sprouting compared with the other 3 ratios. The average length of the sprouting in the 0.04 taper microchannel was significantly longer compared with that in the parallel and 0.06 taper microchannels. Significant differences in HUVEC-GFP sprouting were observed in 5% GelMA hydrogel. Encapsulation of SCAPs within hydrogel further stimulated the sprouting of HUVECs.
Conclusions:
The coculture of SCAPs and HUVECs-GFP at a ratio of 1:1 in 0.04 taper fluidic microchannels fabricated with 5% (w/v) GelMA hydrogel with SCAPs encapsulated was found to be the optimal condition to enhance angiogenesis inside tapered microchannels. |
Persistent Identifier | http://hdl.handle.net/10722/299142 |
ISSN | 2023 Impact Factor: 3.5 2023 SCImago Journal Rankings: 1.356 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Qi, Y | - |
dc.contributor.author | Zou, T | - |
dc.contributor.author | Dissanayaka, WL | - |
dc.contributor.author | Wong, HM | - |
dc.contributor.author | Bertassoni, LE | - |
dc.contributor.author | Zhang, C | - |
dc.date.accessioned | 2021-04-28T02:26:44Z | - |
dc.date.available | 2021-04-28T02:26:44Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Journal of Endodontics, 2021, v. 47 n. 1, p. 52-61 | - |
dc.identifier.issn | 0099-2399 | - |
dc.identifier.uri | http://hdl.handle.net/10722/299142 | - |
dc.description.abstract | Introduction: The transplantation of stem cells/tissue constructs into root canal space is a promising strategy for regenerating lost pulp tissue. However, the root canal system, which is cone shaped with a taper from the larger coronal end to the smaller apical end, limits the vascular supply and, therefore, the regenerative capacity. The current study aimed to fabricate built-in microchannels with different tapers to explore various approaches to endothelialize these microchannels. Methods: The fluidic microchannels with varying tapers (parallel, 0.04, and 0.06) were fabricated within gelatin methacryloyl (GelMA) hydrogel (with or without stem cell from the apical papilla [SCAP] encapsulation) of different concentrations (5%, 7.5%, and 10% [w/v]). Green fluorescent protein–expressing human umbilical vein endothelial cells (HUVECs-GFP) were seeded alone or with SCAPs in coculture into these microchannels. Angiogenic sprouting was assessed by fluorescence and a confocal microscope and ImageJ software (National Institutes of Health, Bethesda, MD). Immunostaining was conducted to illustrate monolayer formation. Data were statistically analyzed by 1-way/2-way analysis of variance. Results: HUVEC-only inoculation formed an endothelial monolayer inside the microchannel without angiogenic sprouting. HUVECs-GFP/SCAPs cocultured at a 1:1 ratio produced the longest sprouting compared with the other 3 ratios. The average length of the sprouting in the 0.04 taper microchannel was significantly longer compared with that in the parallel and 0.06 taper microchannels. Significant differences in HUVEC-GFP sprouting were observed in 5% GelMA hydrogel. Encapsulation of SCAPs within hydrogel further stimulated the sprouting of HUVECs. Conclusions: The coculture of SCAPs and HUVECs-GFP at a ratio of 1:1 in 0.04 taper fluidic microchannels fabricated with 5% (w/v) GelMA hydrogel with SCAPs encapsulated was found to be the optimal condition to enhance angiogenesis inside tapered microchannels. | - |
dc.language | eng | - |
dc.publisher | Elsevier Inc. The Journal's web site is located at http://www.jendodon.com | - |
dc.relation.ispartof | Journal of Endodontics | - |
dc.subject | Angiogenic sprouting | - |
dc.subject | fluidic microchannels | - |
dc.subject | GelMA hydrogel | - |
dc.subject | prevascularization | - |
dc.title | Fabrication of tapered fluidic microchannels conducive to angiogenic sprouting within gelatin methacryloyl hydrogels | - |
dc.type | Article | - |
dc.identifier.email | Wong, HM: wonghmg@hkucc.hku.hk | - |
dc.identifier.email | Zhang, C: zhangcf@hku.hk | - |
dc.identifier.authority | Wong, HM=rp00042 | - |
dc.identifier.authority | Zhang, C=rp01408 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.joen.2020.08.026 | - |
dc.identifier.pmid | 33045266 | - |
dc.identifier.scopus | eid_2-s2.0-85096542827 | - |
dc.identifier.hkuros | 322277 | - |
dc.identifier.volume | 47 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | 52 | - |
dc.identifier.epage | 61 | - |
dc.identifier.isi | WOS:000602701600008 | - |
dc.publisher.place | United States | - |