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- Publisher Website: 10.1002/jbm.a.37017
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Article: Layer-by-layer self‐assembly polyelectrolytes loaded with cyclic adenosine monophosphate enhances the osteo/odontogenic differentiation of stem cells from apical papilla
Title | Layer-by-layer self‐assembly polyelectrolytes loaded with cyclic adenosine monophosphate enhances the osteo/odontogenic differentiation of stem cells from apical papilla |
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Authors | |
Keywords | cell encapsulation layer-by-layer self-assembly stem cell tissue engineering |
Issue Date | 2021 |
Publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0021-9304/ |
Citation | Journal of Biomedical Materials Research Part A, 2021, v. 109 n. 2, p. 207-218 How to Cite? |
Abstract | Cyclic adenosine monophosphate (cAMP) is a second messenger involved in the dental regeneration. However, efficient long-lasting delivery of cAMP that is sufficient to mimic the in vivo microenvironment remains a major challenge. Here, cAMP was loaded in stem cells from apical papilla (SCAPs) using layer-by-layer self-assembly with gelatin and alginate polyelectrolytes (LBL-cAMP-SCAPs). LBL-cAMP-SCAPs expressed cAMP and increased the phosphorylation level of cAMP-response element-binding protein (CREB) which were evaluated by immunofluorescence and western blotting (WB). Enzyme-linked immunosorbent assay (ELISA) demonstrated that a sustained release of cAMP and vascular endothelial growth factor (VEGF) were present up to 14 days. Scanning electron microscopy (SEM) found LBL-coated SCAPs exhibited a spheroid-like morphology. CCK8 and live/dead staining showed that LBL treatment had no significant effect on cell proliferation and viability. LBL-cAMP-SCAPs enhanced mineralized nodule formation and up-regulated the mRNA levels of the osteogenesis-related genes, as well as related transcription factor-2 protein level which were revealed by Alizarin red staining, RT-PCR and WB, respectively. In conclusion, LBL self-assembly loaded with cAMP promoted the osteo/odontogenic differentiation of SCAPs, thereby providing a potential strategy for bioactive molecular delivery in dental regeneration. |
Persistent Identifier | http://hdl.handle.net/10722/298748 |
ISSN | 2023 Impact Factor: 3.9 2023 SCImago Journal Rankings: 0.807 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Zhang, J | - |
dc.contributor.author | Zhao, IS | - |
dc.contributor.author | Yu, OY | - |
dc.contributor.author | Li, QL | - |
dc.contributor.author | Mei, ML | - |
dc.contributor.author | Zhang, C | - |
dc.contributor.author | Chu, CH | - |
dc.date.accessioned | 2021-04-12T03:02:50Z | - |
dc.date.available | 2021-04-12T03:02:50Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Journal of Biomedical Materials Research Part A, 2021, v. 109 n. 2, p. 207-218 | - |
dc.identifier.issn | 1549-3296 | - |
dc.identifier.uri | http://hdl.handle.net/10722/298748 | - |
dc.description.abstract | Cyclic adenosine monophosphate (cAMP) is a second messenger involved in the dental regeneration. However, efficient long-lasting delivery of cAMP that is sufficient to mimic the in vivo microenvironment remains a major challenge. Here, cAMP was loaded in stem cells from apical papilla (SCAPs) using layer-by-layer self-assembly with gelatin and alginate polyelectrolytes (LBL-cAMP-SCAPs). LBL-cAMP-SCAPs expressed cAMP and increased the phosphorylation level of cAMP-response element-binding protein (CREB) which were evaluated by immunofluorescence and western blotting (WB). Enzyme-linked immunosorbent assay (ELISA) demonstrated that a sustained release of cAMP and vascular endothelial growth factor (VEGF) were present up to 14 days. Scanning electron microscopy (SEM) found LBL-coated SCAPs exhibited a spheroid-like morphology. CCK8 and live/dead staining showed that LBL treatment had no significant effect on cell proliferation and viability. LBL-cAMP-SCAPs enhanced mineralized nodule formation and up-regulated the mRNA levels of the osteogenesis-related genes, as well as related transcription factor-2 protein level which were revealed by Alizarin red staining, RT-PCR and WB, respectively. In conclusion, LBL self-assembly loaded with cAMP promoted the osteo/odontogenic differentiation of SCAPs, thereby providing a potential strategy for bioactive molecular delivery in dental regeneration. | - |
dc.language | eng | - |
dc.publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0021-9304/ | - |
dc.relation.ispartof | Journal of Biomedical Materials Research Part A | - |
dc.rights | This is the peer reviewed version of the following article: Journal of Biomedical Materials Research Part A, 2021, v. 109 n. 2, p. 207-218, which has been published in final form at https://doi.org/10.1002/jbm.a.37017. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. | - |
dc.subject | cell encapsulation | - |
dc.subject | layer-by-layer | - |
dc.subject | self-assembly | - |
dc.subject | stem cell | - |
dc.subject | tissue engineering | - |
dc.title | Layer-by-layer self‐assembly polyelectrolytes loaded with cyclic adenosine monophosphate enhances the osteo/odontogenic differentiation of stem cells from apical papilla | - |
dc.type | Article | - |
dc.identifier.email | Yu, OY: ollieyu@hku.hk | - |
dc.identifier.email | Zhang, C: zhangcf@hku.hk | - |
dc.identifier.email | Chu, CH: chchu@hku.hk | - |
dc.identifier.authority | Yu, OY=rp02658 | - |
dc.identifier.authority | Zhang, C=rp01408 | - |
dc.identifier.authority | Chu, CH=rp00022 | - |
dc.description.nature | postprint | - |
dc.identifier.doi | 10.1002/jbm.a.37017 | - |
dc.identifier.pmid | 32441418 | - |
dc.identifier.scopus | eid_2-s2.0-85087662247 | - |
dc.identifier.hkuros | 322031 | - |
dc.identifier.volume | 109 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 207 | - |
dc.identifier.epage | 218 | - |
dc.identifier.isi | WOS:000546410600001 | - |
dc.publisher.place | United States | - |