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Article: Longitudinal profile of retinal ganglion cell damage assessed with blue-light confocal scanning laser ophthalmoscopy after ischaemic reperfusion injury

TitleLongitudinal profile of retinal ganglion cell damage assessed with blue-light confocal scanning laser ophthalmoscopy after ischaemic reperfusion injury
Authors
Issue Date2009
Citation
British Journal of Ophthalmology, 2009, v. 93, n. 7, p. 964-968 How to Cite?
AbstractAim: To longitudinally investigate retinal ganglion cell (RGC) expression of Thy-1, a cell-surface glycoprotein specifically expressed in RGCs, with a blue-light confocal scanning laser ophthalmoscope, following retinal ischaemia induced by acute elevation of intraocular pressure. Methods: A blue-light confocal scanning laser ophthalmoscope (bCSLO, 460 nm excitation and 490 nm detection) was used to image Thy1-cyan fluorescent protein (CFP) mice before and weekly for 4 weeks after transiently elevating the intraocular pressure to 115 mm Hg for 45 min (n = 4) or 90 min (n = 5) to induce ischaemic injury. Corresponding retinal areas before and after the intraocular pressure (IOP) elevation, during the period of ischaemic reperfusion, were compared, and the fluorescent spots (Thy-1 expressing RGCs) were counted. The longitudinal profile of CFP-expressing RGCs was modelled with a linear regression equation. The spatial distribution of RGC damage was analysed in the superior, nasal, inferior and temporal quadrants of the retina. Results: No significant change was found at 4 weeks after 45 min of IOP elevation (n = 4, p =0 .465). The average RGC densities before and 4 weeks after IOP elevation were 1660 (SD 242) cells/mm2 and 1624 (209) cells/mm2, respectively. However, significant loss of CFP-expressing RGCs was detected at 1 week following 90 min of IOP elevation (n = 5, p<0.001). After this initial RGC loss, no significant change was detected subsequently. The proportion of RGC fluorescence remaining was variable and ranged from 14.5% to 79.5% at 4 weeks after the IOP elevation. The average RGC densities before and 4 weeks after IOP elevation were 1443 (162) cells/ mm2 and 680 (385) cells/mm2, respectively. Diffuse loss of fluorescent RGCs was observed in the spatial distribution analysis. Conclusions: The longitudinal profile of Thy-1 expressing RGC fluorescence loss after ischaemic injury is nonprogressive and unrelated to the duration of reperfusion.
Persistent Identifierhttp://hdl.handle.net/10722/298482
ISSN
2023 Impact Factor: 3.7
2023 SCImago Journal Rankings: 1.862
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLeung, C. K.S.-
dc.contributor.authorLindsey, J. D.-
dc.contributor.authorChen, L.-
dc.contributor.authorLiu, Q.-
dc.contributor.authorWeinreb, R. N.-
dc.date.accessioned2021-04-08T03:08:31Z-
dc.date.available2021-04-08T03:08:31Z-
dc.date.issued2009-
dc.identifier.citationBritish Journal of Ophthalmology, 2009, v. 93, n. 7, p. 964-968-
dc.identifier.issn0007-1161-
dc.identifier.urihttp://hdl.handle.net/10722/298482-
dc.description.abstractAim: To longitudinally investigate retinal ganglion cell (RGC) expression of Thy-1, a cell-surface glycoprotein specifically expressed in RGCs, with a blue-light confocal scanning laser ophthalmoscope, following retinal ischaemia induced by acute elevation of intraocular pressure. Methods: A blue-light confocal scanning laser ophthalmoscope (bCSLO, 460 nm excitation and 490 nm detection) was used to image Thy1-cyan fluorescent protein (CFP) mice before and weekly for 4 weeks after transiently elevating the intraocular pressure to 115 mm Hg for 45 min (n = 4) or 90 min (n = 5) to induce ischaemic injury. Corresponding retinal areas before and after the intraocular pressure (IOP) elevation, during the period of ischaemic reperfusion, were compared, and the fluorescent spots (Thy-1 expressing RGCs) were counted. The longitudinal profile of CFP-expressing RGCs was modelled with a linear regression equation. The spatial distribution of RGC damage was analysed in the superior, nasal, inferior and temporal quadrants of the retina. Results: No significant change was found at 4 weeks after 45 min of IOP elevation (n = 4, p =0 .465). The average RGC densities before and 4 weeks after IOP elevation were 1660 (SD 242) cells/mm2 and 1624 (209) cells/mm2, respectively. However, significant loss of CFP-expressing RGCs was detected at 1 week following 90 min of IOP elevation (n = 5, p<0.001). After this initial RGC loss, no significant change was detected subsequently. The proportion of RGC fluorescence remaining was variable and ranged from 14.5% to 79.5% at 4 weeks after the IOP elevation. The average RGC densities before and 4 weeks after IOP elevation were 1443 (162) cells/ mm2 and 680 (385) cells/mm2, respectively. Diffuse loss of fluorescent RGCs was observed in the spatial distribution analysis. Conclusions: The longitudinal profile of Thy-1 expressing RGC fluorescence loss after ischaemic injury is nonprogressive and unrelated to the duration of reperfusion.-
dc.languageeng-
dc.relation.ispartofBritish Journal of Ophthalmology-
dc.titleLongitudinal profile of retinal ganglion cell damage assessed with blue-light confocal scanning laser ophthalmoscopy after ischaemic reperfusion injury-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1136/bjo.2008.150482-
dc.identifier.pmid19224902-
dc.identifier.pmcidPMC5499383-
dc.identifier.scopuseid_2-s2.0-67649655581-
dc.identifier.volume93-
dc.identifier.issue7-
dc.identifier.spage964-
dc.identifier.epage968-
dc.identifier.eissn1468-2079-
dc.identifier.isiWOS:000267341900026-
dc.identifier.issnl0007-1161-

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