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- Publisher Website: 10.1167/iovs.07-1447
- Scopus: eid_2-s2.0-56149113407
- PMID: 18441315
- WOS: WOS:000260502200030
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Article: Longitudinal profile of retinal ganglion cell damage after optic nerve crush with blue-light confocal scanning laser ophthalmoscopy
Title | Longitudinal profile of retinal ganglion cell damage after optic nerve crush with blue-light confocal scanning laser ophthalmoscopy |
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Authors | |
Issue Date | 2008 |
Citation | Investigative Ophthalmology and Visual Science, 2008, v. 49, n. 11, p. 4898-4902 How to Cite? |
Abstract | PURPOSE. To investigate the long-term longitudinal profile of retinal ganglion cell (RGC) damage after optic nerve crush with a new technique for in vivo imaging of RGCs. METHODS. A blue-light confocal scanning laser ophthalmoscope (bCSLO; 460 nm excitation, 490 nm detection) was used to image Thy-1 CFP mice aged 6 to 9 months (n = 5) before optic nerve crush, weekly after crush for 3 weeks, and at weeks 10 and 50 after optic nerve crush. A sham procedure was performed in the contralateral eye, and it was imaged as a control. Corresponding retinal areas before and after optic nerve crush were compared, and the fluorescent spots were counted manually. The longitudinal profile of RGC degeneration was modeled and compared with one-phase and two-phase exponential decay equations. RESULTS. A significant and progressive loss of fluorescent spots was found after optic nerve crush with 18.6% ± 2.3%, 11.3% ± 3.4%, 8.8% ± 5.3%, 4.2% ± 3.1%, and 3.3% ± 2.1% of Thy-1-expressing RGCs remaining at weeks 1, 2, 3, 10, and 50, respectively, after optic nerve crush (P < 0.001; n = 5). There was no change in the fluorescence density in the contralateral control (P = 0.893). Two-phase exponential decay (y = 0.03 + 0.83e + 0.14e ) was a better fit than one-phase exponential decay (y = 0.94e + 0.06; P = 0.003) equations, with half-lives of fast phase and slow phase of 1.7 days and 16.3 days, respectively. CONCLUSIONS. The longitudinal profile of RGC degeneration after optic nerve crush is characterized by a two-phase exponential decay model. bCSLO imaging provides an efficient and noninvasive approach to the longitudinal study of progressive RGC damage. Copyright © Association for Research in Vision and Ophthalmology. -2.78t -0.30t -1.93t |
Persistent Identifier | http://hdl.handle.net/10722/298465 |
ISSN | 2023 Impact Factor: 5.0 2023 SCImago Journal Rankings: 1.422 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Leung, Christopher Kai Shun | - |
dc.contributor.author | Lindsey, James D. | - |
dc.contributor.author | Crowston, Jonathan G. | - |
dc.contributor.author | Lijia, Chen | - |
dc.contributor.author | Chiang, Sylvia | - |
dc.contributor.author | Weinreb, Robert N. | - |
dc.date.accessioned | 2021-04-08T03:08:28Z | - |
dc.date.available | 2021-04-08T03:08:28Z | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | Investigative Ophthalmology and Visual Science, 2008, v. 49, n. 11, p. 4898-4902 | - |
dc.identifier.issn | 0146-0404 | - |
dc.identifier.uri | http://hdl.handle.net/10722/298465 | - |
dc.description.abstract | PURPOSE. To investigate the long-term longitudinal profile of retinal ganglion cell (RGC) damage after optic nerve crush with a new technique for in vivo imaging of RGCs. METHODS. A blue-light confocal scanning laser ophthalmoscope (bCSLO; 460 nm excitation, 490 nm detection) was used to image Thy-1 CFP mice aged 6 to 9 months (n = 5) before optic nerve crush, weekly after crush for 3 weeks, and at weeks 10 and 50 after optic nerve crush. A sham procedure was performed in the contralateral eye, and it was imaged as a control. Corresponding retinal areas before and after optic nerve crush were compared, and the fluorescent spots were counted manually. The longitudinal profile of RGC degeneration was modeled and compared with one-phase and two-phase exponential decay equations. RESULTS. A significant and progressive loss of fluorescent spots was found after optic nerve crush with 18.6% ± 2.3%, 11.3% ± 3.4%, 8.8% ± 5.3%, 4.2% ± 3.1%, and 3.3% ± 2.1% of Thy-1-expressing RGCs remaining at weeks 1, 2, 3, 10, and 50, respectively, after optic nerve crush (P < 0.001; n = 5). There was no change in the fluorescence density in the contralateral control (P = 0.893). Two-phase exponential decay (y = 0.03 + 0.83e + 0.14e ) was a better fit than one-phase exponential decay (y = 0.94e + 0.06; P = 0.003) equations, with half-lives of fast phase and slow phase of 1.7 days and 16.3 days, respectively. CONCLUSIONS. The longitudinal profile of RGC degeneration after optic nerve crush is characterized by a two-phase exponential decay model. bCSLO imaging provides an efficient and noninvasive approach to the longitudinal study of progressive RGC damage. Copyright © Association for Research in Vision and Ophthalmology. -2.78t -0.30t -1.93t | - |
dc.language | eng | - |
dc.relation.ispartof | Investigative Ophthalmology and Visual Science | - |
dc.title | Longitudinal profile of retinal ganglion cell damage after optic nerve crush with blue-light confocal scanning laser ophthalmoscopy | - |
dc.type | Article | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1167/iovs.07-1447 | - |
dc.identifier.pmid | 18441315 | - |
dc.identifier.pmcid | PMC5557086 | - |
dc.identifier.scopus | eid_2-s2.0-56149113407 | - |
dc.identifier.volume | 49 | - |
dc.identifier.issue | 11 | - |
dc.identifier.spage | 4898 | - |
dc.identifier.epage | 4902 | - |
dc.identifier.eissn | 1552-5783 | - |
dc.identifier.isi | WOS:000260502200030 | - |
dc.identifier.issnl | 0146-0404 | - |