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- Publisher Website: 10.1038/ja.2009.34
- Scopus: eid_2-s2.0-68149160029
- PMID: 19444301
- WOS: WOS:000268266500005
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Article: A cell-based screening system for detection of inhibitors toward mycobacterial cell wall core
Title | A cell-based screening system for detection of inhibitors toward mycobacterial cell wall core |
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Authors | |
Keywords | Screening system Cell wall core Corynebacterium glutamicum Mycolic acid Mycobacterium tuberculosis |
Issue Date | 2009 |
Citation | Journal of Antibiotics, 2009, v. 62, n. 6, p. 315-318 How to Cite? |
Abstract | Mycobacterium tuberculosis and nonpathogenic bacteria, Corynebacterium glutamicum, possess a common and unusual cell wall architecture. A cell-based screening system was designed to identify novel compounds interacting with the synthesis, assembly or regulation of the M. tuberculosis cell wall. C. glutamicum was tested in a paired medium assay in 96-well plates with natural product extracts and pure chemical compounds in the presence and absence of the osmotic stabilizer, sorbitol and some ions. Growth was visually examined over a 12-h period and detected with a microplate reader for absorbance at 544 nm. Screening hits from the osmotic stabilizer rescue were then examined by mycolic acid analysis to confirm the effect on cell wall integrity. © 2009 Japan Antibiotics Research Association All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/298425 |
ISSN | 2023 Impact Factor: 2.1 2023 SCImago Journal Rankings: 0.546 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Gao, Peng | - |
dc.contributor.author | Guan, Yan | - |
dc.contributor.author | Song, Danqing | - |
dc.contributor.author | Xiao, Chunling | - |
dc.date.accessioned | 2021-04-08T03:08:23Z | - |
dc.date.available | 2021-04-08T03:08:23Z | - |
dc.date.issued | 2009 | - |
dc.identifier.citation | Journal of Antibiotics, 2009, v. 62, n. 6, p. 315-318 | - |
dc.identifier.issn | 0021-8820 | - |
dc.identifier.uri | http://hdl.handle.net/10722/298425 | - |
dc.description.abstract | Mycobacterium tuberculosis and nonpathogenic bacteria, Corynebacterium glutamicum, possess a common and unusual cell wall architecture. A cell-based screening system was designed to identify novel compounds interacting with the synthesis, assembly or regulation of the M. tuberculosis cell wall. C. glutamicum was tested in a paired medium assay in 96-well plates with natural product extracts and pure chemical compounds in the presence and absence of the osmotic stabilizer, sorbitol and some ions. Growth was visually examined over a 12-h period and detected with a microplate reader for absorbance at 544 nm. Screening hits from the osmotic stabilizer rescue were then examined by mycolic acid analysis to confirm the effect on cell wall integrity. © 2009 Japan Antibiotics Research Association All rights reserved. | - |
dc.language | eng | - |
dc.relation.ispartof | Journal of Antibiotics | - |
dc.subject | Screening system | - |
dc.subject | Cell wall core | - |
dc.subject | Corynebacterium glutamicum | - |
dc.subject | Mycolic acid | - |
dc.subject | Mycobacterium tuberculosis | - |
dc.title | A cell-based screening system for detection of inhibitors toward mycobacterial cell wall core | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1038/ja.2009.34 | - |
dc.identifier.pmid | 19444301 | - |
dc.identifier.scopus | eid_2-s2.0-68149160029 | - |
dc.identifier.volume | 62 | - |
dc.identifier.issue | 6 | - |
dc.identifier.spage | 315 | - |
dc.identifier.epage | 318 | - |
dc.identifier.isi | WOS:000268266500005 | - |
dc.identifier.issnl | 0021-8820 | - |